BBa_K2088001 1 BBa_K2088001 copA promoter 2016-09-22T11:00:00Z 2016-09-23T08:37:16Z genomic DNA CopA, the principal copper efflux ATPase in Escherichia coli, is induced by elevated copper in the medium.The copA promoter is Copper-responsive and regulated by CueR,a Member of the MerR Family. false false _2556_ 33447 33447 9 false no false chenrui qin BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K2088672 1 BBa_K2088672 copA + riboJ +mhbDHIM + terminator 2016-10-08T11:00:00Z 2016-10-20T11:41:00Z This is a composite part including gene cluster(mhbDHIM) encoding the enzymes involved in the catabolism of 3-HBA to fumarate and pyruvate. This composite part uses a promoter induced by copper ions(BBa_K2088001&#65289;&#65292; riboJ&#65288;BBa_K1679038&#65289;&#65292; mhbD with native rbs&#65288;BBa_K2088667&#65289;, mhbH with native rbs(BBa_K2088670), mhbI with native rbs(BBa_K2088669), mhbM with native rbs(BBa_K2088668), and a double terminator(BBa_B0015). This part is used to degrade 3-hydroxybenzoate(3-HBA) to fumarate and pyruvate via the gentisate pathway induced by copper iron. false false _2556_ 33751 33751 9 true Used riboJ to help expose the rbs. false Xia Chen component2525922 1 BBa_B0015 component2525915 1 BBa_K2088692 component2525905 1 BBa_K2088001 component2525906 1 BBa_K2066506 annotation2525922 1 BBa_B0015 range2525922 1 3965 4093 annotation2525905 1 BBa_K2088001 range2525905 1 1 242 annotation2525915 1 BBa_K2088692 range2525915 1 324 3964 annotation2525906 1 BBa_K2066506 range2525906 1 243 323 BBa_K2088692 1 BBa_K2088692 gene cluster mhbDHIM 2016-10-19T11:00:00Z 2016-10-20T11:39:43Z Genes from Klebsiella pneumoniae M5a1. This gene cluster encodes the enzymes involved in the catabolism of 3-hydroxybenzoate to fumarate and pyruvate via the gentisate pathway. mhbD, mhbH, mhbI and mhbM genes encode the gentisate 1,2-dioxygenase, maleylpyruvate isomerase, fumarylpyruvate hydratase and 3-HBA 6-hydroxylase respectively. false false _2556_ 33751 33751 9 false There are 3 site ???directed mutagenesis in this gene cluster. false Xia Chen annotation2525900 1 mhbI range2525900 1 1795 2439 annotation2525898 1 mhbH range2525898 1 1088 1786 annotation2525901 1 native rbs of mhbM range2525901 1 2440 2447 annotation2525735 1 native rbs of mhbD range2525735 1 1 25 annotation2525899 1 native rbs of mhbI range2525899 1 1787 1794 annotation2525902 1 mhbM range2525902 1 2448 3641 annotation2525736 1 mhbD range2525736 1 26 1063 annotation2525737 1 native rbs of mhbH range2525737 1 1064 1087 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K2066506 1 BBa_K2066506 RiboJ (Ribozyme Insulator) Lou et. al. 2012 2016-08-30T11:00:00Z 2016-10-12T12:27:33Z Part sequence is from Lou et al. 2012, Supplemental Section V (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???). RiboJ is the sequence for a ribozyme studied in Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context"). WM iGEM 2016 used this sequence between the promoter and ribosome sequence. One of our goals for using this part is moving it onto a Biobrick backbone. Furthermore, In Lou et. al, this ribozyme sequence was said to act as an insulator which generalizes protein expression levels for a given promoter. We used RiboJ to collect data for our Ribozyme characterization project as well as our ribosome and promoter characterization projects. false false _2534_ 27446 31541 9 false We designed this part to use as an insulator and also move this riboJ sequence onto a Biobrick backbone. false Likhitha Kolla BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2088001_sequence 1 cctttttatagatgcgggaggtaattcctcaccccggtgccgattttcaggcatcctgatttaacttagcacccgcaacttaactacaggaaaacaaagagataaatgtctaatcctgatgcaaatcgagccgattttttaatctttacggacttttacccgcctggtttattaatttcttgaccttccccttgctggaaggtttaacctttatcacagccagtcaaaactgtcttaaaggt BBa_K2066506_sequence 1 agctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaaactaga BBa_K2088672_sequence 1 cctttttatagatgcgggaggtaattcctcaccccggtgccgattttcaggcatcctgatttaacttagcacccgcaacttaactacaggaaaacaaagagataaatgtctaatcctgatgcaaatcgagccgattttttaatctttacggacttttacccgcctggtttattaatttcttgaccttccccttgctggaaggtttaacctttatcacagccagtcaaaactgtcttaaaggtagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaaactagattgtgaataaataaggagaacgcttatgtcccagtccaccacggaagcaaataacggccgtcagcagttttaccagcatatttccgggcaaaacctgacgccgctttgggaatcgctgcaccatctggtgccgaaaacgccgaacgccacctgcgcgccggcttactggaattatcaggagattcgcccgctgctgctggaaagcggcaagctgattggcgcgaaagaggcgatacgccgcgtgctggtgctggaaaacccggcgctgcgcgggcagtcgtcgattacctcctcgctgtacgcgggtttgcagctgattatgcccggcgaagtggcgccgagccatcggcacaaccagtcggcgctgcgctttgtcgtggaaggcgaaggggcgtttaccgccgttgacggcgaacggaccgccatgcgcgccggcgactttattctgacgccgcagtggcgctggcacgatcacggcaaccccggcaacgaaccggttatctggctcgacggcctcgatctgccgctggtgaactatctgggctgcggcttcgcggaggactatccgcaagatcagcagccggtgacccgcaaagagggggattatctgccgcgctacgccgccaacatgctgccgctgcgccatcagtccggtaactcttcgcccatctttaactatcgctacgaccgcagccgcgaagcgctgcacgatctgacgcggatgggcgatgccgacgagtgggatggctataagatgcgctacgtcaacccggtcaccggcggctacccgatgccgtcgatgggcgccttcctgcaactgttaccgaaagggtttacctcgcgagcggcgaaaaccaccgacagtaccgtctatcacgtggtggaaggcagcggccaggtcaccattggcgaacagaccttcgcttttcaggcaaaagatatcttcgtggtgccgacctggcacgccgtctcttttatctccgccgaagatagcgtgttattcagcttttcggaccgtcccgtgcaggaagcgctcggcctgttccgcgaagcgcgttattaaaaattaagaataaggaatcgaatcatgactcaatacgtttttgcaccccaggctccgattagcgttccggttgtcggcagcgatgagcagttcccggtgcgccgcgtttactgcgtgggccgcaactacgccgctcacgcgcgtgaaatgggctttgacccggatcgcgaaccgccgttcttcttctgcaagcctgccgatgcggtggtgcccgtcgccgcgggagaaacgctgaacctgccgtatccgacgcaaaccgataactatcactacgaaattgagctggtggtggcgattggcaagcggggaagcgatatcccgctggagcaggcccatgagtatatctggggctatgccacggggctggatatgacccgccgcgatcgccagatggagatgcgccagatgggccgtccgtgggagattggtaaagcttttgacctttccgcgccgattgcgccgctgcataaagcggatacggcggacgtgagccaggccggaatctggctgcaagtaaacggggaagatcatcagcgcagcgatattcgccacctgatctggtcggttaacgaaaccatcagctatctttccggtttctttgagctgcaacccggcgatctgattttcaccggcacgccggaaggcgtcggcgcggtggtgaaaggcgatgtcatcaccggcaacgtcgatggactgacgccaatcgccgtgcgcgtggtgtgaggtgaatgatgaagctgtacagtttttttaatagctcagcgtcctaccgcgtgcggattgcgctggcgctgaaaggcatcgactaccagagcgtcggcgttaacatccgcatcggccagcagaacgcgctggagtaccggcggctgaacccggtcggtctggtgccgacgctcattaccgacaaaggcgaatctctcggccagtcgctggcgattgccgactggctggaccggcactatccgcagccgctgctgctgccccaggcggacagcgcgcggatgcgggtactggagatcgtttacgccatcgcctgcgatattcatcccattaataacatgcgcgttttacgctaccttggcgacgagctaaaggtaagcgaagaggagaaaaaacgctggtatgcgcactggatccagcagggattcagcgccgtcgagcagctattgcgccacgcgaaatccggcgatttttgcgtgggcgatgcgcctaccctggcggactgctgcctggtgccgcagtgggccaacgccctgaggatgggctgcgatctcagccactatccgcgctgccaggcggtttatgacgcctgtacccggctgccggcatttatcgccgccgcgccggaaaaccaacaagataagatcccggcttaggaggcaacatggcaaaagtgatgcgagcaattattgtcggcggcggtattggcggagcggccacggcgctctctctggcgcgccagggcatcaaagtgatgctgctggaaaaggcgcatgaaatcggcgaaatcggcgccgggatccagttgggtccaaacgcattctcggcgctcgacagcctcggcgtcggcgaagtcgcccgccagcgcgcggtgtttaccgaccatatcaccatgatggatgcggtcaacggcgaagaggtggtgcatattgagaccggacaggccttccgcgaccattttggcggaccctatgcggtgatccatcgggtggatatccacgccaccgtctgggaggcggcgctgacccatccggccgtcgagtaccgtacttcgacgcaggtagtggatatccgccagaccgccgatgacgtgacggtgtttgatgataaaggcaacagctggacggcggatattctgatcggctgcgacggcggtaagtcggtggtgcgccaaagtctgctgggcgattcgccgcgggtaaccggccatgtcgtttatcgcgcggtggttgacgccgccgatatgccggacgatttacgcatcaacgcgccggtgctgtgggccggtccgcactgccacctggtgcattatccgctgcgcggcggcaagcagtacaacctggtggtgaccttccatagtcggcagcaggaagagtggggcgtgcgcgacggcagtaaagaagaggtgctctcctactttaaaggcatccacccgcgtccccggcagatgctggacaaaccgacctcctggcgtcgctggtccaccgccgaccgcgagccggtagagaagtggggcaacgaccgcatcacgctggttggcgatgccgcgcatccggtggcgcagtatatggcccagggcgcctgtatggcgctggaagatgcggtgacgctgggtaaagcgctggcgcaatgcgacggcgacgccgcccgcgctttcgcgctgtatgaatcggtacgtattccgcgtaccgcgcgcatcgtctggtcgacgcgggaaatggggcgggtctatcacgccgccggcgttgagcgtcaggtgcgcaatctgctgtggaaaggcaaaacccagagcgagttttatcgcggcatcgagtggctgtacggctggaaagaggataactgcctggaagcgcgttaaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2088692_sequence 1 ttgtgaataaataaggagaacgcttatgtcccagtccaccacggaagcaaataacggccgtcagcagttttaccagcatatttccgggcaaaacctgacgccgctttgggaatcgctgcaccatctggtgccgaaaacgccgaacgccacctgcgcgccggcttactggaattatcaggagattcgcccgctgctgctggaaagcggcaagctgattggcgcgaaagaggcgatacgccgcgtgctggtgctggaaaacccggcgctgcgcgggcagtcgtcgattacctcctcgctgtacgcgggtttgcagctgattatgcccggcgaagtggcgccgagccatcggcacaaccagtcggcgctgcgctttgtcgtggaaggcgaaggggcgtttaccgccgttgacggcgaacggaccgccatgcgcgccggcgactttattctgacgccgcagtggcgctggcacgatcacggcaaccccggcaacgaaccggttatctggctcgacggcctcgatctgccgctggtgaactatctgggctgcggcttcgcggaggactatccgcaagatcagcagccggtgacccgcaaagagggggattatctgccgcgctacgccgccaacatgctgccgctgcgccatcagtccggtaactcttcgcccatctttaactatcgctacgaccgcagccgcgaagcgctgcacgatctgacgcggatgggcgatgccgacgagtgggatggctataagatgcgctacgtcaacccggtcaccggcggctacccgatgccgtcgatgggcgccttcctgcaactgttaccgaaagggtttacctcgcgagcggcgaaaaccaccgacagtaccgtctatcacgtggtggaaggcagcggccaggtcaccattggcgaacagaccttcgcttttcaggcaaaagatatcttcgtggtgccgacctggcacgccgtctcttttatctccgccgaagatagcgtgttattcagcttttcggaccgtcccgtgcaggaagcgctcggcctgttccgcgaagcgcgttattaaaaattaagaataaggaatcgaatcatgactcaatacgtttttgcaccccaggctccgattagcgttccggttgtcggcagcgatgagcagttcccggtgcgccgcgtttactgcgtgggccgcaactacgccgctcacgcgcgtgaaatgggctttgacccggatcgcgaaccgccgttcttcttctgcaagcctgccgatgcggtggtgcccgtcgccgcgggagaaacgctgaacctgccgtatccgacgcaaaccgataactatcactacgaaattgagctggtggtggcgattggcaagcggggaagcgatatcccgctggagcaggcccatgagtatatctggggctatgccacggggctggatatgacccgccgcgatcgccagatggagatgcgccagatgggccgtccgtgggagattggtaaagcttttgacctttccgcgccgattgcgccgctgcataaagcggatacggcggacgtgagccaggccggaatctggctgcaagtaaacggggaagatcatcagcgcagcgatattcgccacctgatctggtcggttaacgaaaccatcagctatctttccggtttctttgagctgcaacccggcgatctgattttcaccggcacgccggaaggcgtcggcgcggtggtgaaaggcgatgtcatcaccggcaacgtcgatggactgacgccaatcgccgtgcgcgtggtgtgaggtgaatgatgaagctgtacagtttttttaatagctcagcgtcctaccgcgtgcggattgcgctggcgctgaaaggcatcgactaccagagcgtcggcgttaacatccgcatcggccagcagaacgcgctggagtaccggcggctgaacccggtcggtctggtgccgacgctcattaccgacaaaggcgaatctctcggccagtcgctggcgattgccgactggctggaccggcactatccgcagccgctgctgctgccccaggcggacagcgcgcggatgcgggtactggagatcgtttacgccatcgcctgcgatattcatcccattaataacatgcgcgttttacgctaccttggcgacgagctaaaggtaagcgaagaggagaaaaaacgctggtatgcgcactggatccagcagggattcagcgccgtcgagcagctattgcgccacgcgaaatccggcgatttttgcgtgggcgatgcgcctaccctggcggactgctgcctggtgccgcagtgggccaacgccctgaggatgggctgcgatctcagccactatccgcgctgccaggcggtttatgacgcctgtacccggctgccggcatttatcgccgccgcgccggaaaaccaacaagataagatcccggcttaggaggcaacatggcaaaagtgatgcgagcaattattgtcggcggcggtattggcggagcggccacggcgctctctctggcgcgccagggcatcaaagtgatgctgctggaaaaggcgcatgaaatcggcgaaatcggcgccgggatccagttgggtccaaacgcattctcggcgctcgacagcctcggcgtcggcgaagtcgcccgccagcgcgcggtgtttaccgaccatatcaccatgatggatgcggtcaacggcgaagaggtggtgcatattgagaccggacaggccttccgcgaccattttggcggaccctatgcggtgatccatcgggtggatatccacgccaccgtctgggaggcggcgctgacccatccggccgtcgagtaccgtacttcgacgcaggtagtggatatccgccagaccgccgatgacgtgacggtgtttgatgataaaggcaacagctggacggcggatattctgatcggctgcgacggcggtaagtcggtggtgcgccaaagtctgctgggcgattcgccgcgggtaaccggccatgtcgtttatcgcgcggtggttgacgccgccgatatgccggacgatttacgcatcaacgcgccggtgctgtgggccggtccgcactgccacctggtgcattatccgctgcgcggcggcaagcagtacaacctggtggtgaccttccatagtcggcagcaggaagagtggggcgtgcgcgacggcagtaaagaagaggtgctctcctactttaaaggcatccacccgcgtccccggcagatgctggacaaaccgacctcctggcgtcgctggtccaccgccgaccgcgagccggtagagaagtggggcaacgaccgcatcacgctggttggcgatgccgcgcatccggtggcgcagtatatggcccagggcgcctgtatggcgctggaagatgcggtgacgctgggtaaagcgctggcgcaatgcgacggcgacgccgcccgcgctttcgcgctgtatgaatcggtacgtattccgcgtaccgcgcgcatcgtctggtcgacgcgggaaatggggcgggtctatcacgccgccggcgttgagcgtcaggtgcgcaatctgctgtggaaaggcaaaacccagagcgagttttatcgcggcatcgagtggctgtacggctggaaagaggataactgcctggaagcgcgttaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z