BBa_K2088673 1 BBa_K2088673 copA+RiboJ+GFP 2016-10-08T11:00:00Z 2016-10-09T07:10:01Z We get the gene sequence from http://parts.igem.org/Part:BBa_K1555000 and http://parts.igem.org/Part:BBa_K1679038. We synthetised this part. copA is a copper-sensitive promoter and RiboJ((a section of functional RNA used to buffer synthetic circuits from genetic context) and we use GFP as the reporter. We want to use copper as the inducer to make the following ORF work. true false _2556_ 30833 30833 9 false We want to use RiboJ to block the interaction between copA promoter and RBS(B0030) so we did not add any other base between them. false Ping Xiaofei component2489047 1 BBa_K1555000 component2489048 1 BBa_K1679038 annotation2489047 1 BBa_K1555000 range2489047 1 1 1127 annotation2489048 1 BBa_K1679038 range2489048 1 1136 1210 BBa_K1679038 1 BBa_K1679038 RiboJ 2015-09-17T11:00:00Z 2015-09-18T01:15:04Z Synthesized by Genewiz. After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme and an additional 23-nt hairpin immediately downstream to help expose the RBS. STRSV can cleaves the mRNA autocatalytically at a defined residue, which had the effect of cleaving extraneous RNA leaders that arose from transcription from promoters with different start sites.[1] Attributed to the biochemical function mentioned above, RiboJ has insulating capability. We use it to buffer synthetic circuits from genetic context. [1] Lou C, Stanton B, Chen Y J, et al. Ribozyme-based insulator parts buffer synthetic circuits from genetic context[J]. Nature biotechnology, 2012, 30(11): 1137-1142. false false _2097_ 25072 25072 9 false After transcription, RiboJ becomes a section of functional RNA which comprises the sTRSV- ribozyme and an additional 23-nt hairpin. Users can insert it into promoter and RBS to reduce noise caused by upstream sequence and stabilize mRNA. false Xihan Zhang BBa_K1555000 1 BBa_K1555000 The copa promoter wth a gfp reporter 2014-09-27T11:00:00Z 2015-05-08T01:10:53Z We attained this sensor from E.coli's genome. This part is our testing part.The copa is one of the candidates of our copper sensor.For comparing with other two sequences,marRAB and cueo,we linked a reporter(BBa_E0840) follow the promoter of copa.With the concentration of copper rises, the expression of downstream gfp coding gene increases.We designed two experiments to test those candidates' sensitivity and specificity individually by measure fluorescence intensity. false false _1940_ 0 22116 9 In stock false For guaranteeing our sensor is simple but copper-sensitive,we need to find a operator which directly or indirectly regulated by copper in copa sequence. false Xu Xuan annotation2387952 1 BBa_B0010 range2387952 1 999 1078 annotation2387957 1 stop range2387957 1 1120 1120 annotation2387948 1 RBS range2387948 1 257 261 annotation2387954 1 BBa_B0012 range2387954 1 1087 1127 annotation2387949 1 BBa_E0040 range2387949 1 271 990 annotation2387951 1 BBa_B0030 range2387951 1 250 264 annotation2387953 1 stem_loop range2387953 1 1010 1053 annotation2387950 1 RBS-1\Strong range2387950 1 250 264 annotation2387955 1 T7 TE range2387955 1 1049 1113 annotation2387946 1 copa promoter range2387946 1 1 241 annotation2387956 1 polya range2387956 1 1113 1127 annotation2387947 1 BBa_E0840 range2387947 1 250 1127 BBa_K2088673_sequence 1 cctttttatagatgcgggaggtaattcctcaccccggtgccgattttcaggcatcctgatttaacttagcacccgcaacttaactacaggaaaacaaagagataaatgtctaatcctgatgcaaatcgagccgattttttaatctttacggacttttacccgcctggtttattaatttcttgaccttccccttgctggaaggtttaacctttatcacagccagtcaaaactgtcttaaaggtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttaaacaaaattatttgtagaggctgtttcgtcctcacggactcatcagaccggaaagcacatccggtgacagct BBa_K1555000_sequence 1 cctttttatagatgcgggaggtaattcctcaccccggtgccgattttcaggcatcctgatttaacttagcacccgcaacttaactacaggaaaacaaagagataaatgtctaatcctgatgcaaatcgagccgattttttaatctttacggacttttacccgcctggtttattaatttcttgaccttccccttgctggaaggtttaacctttatcacagccagtcaaaactgtcttaaaggtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1679038_sequence 1 ttaaacaaaattatttgtagaggctgtttcgtcctcacggactcatcagaccggaaagcacatccggtgacagct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z