LOCUS       BBa_K208889             1811 bp    DNA     linear       17-May-2024
DEFINITION  RBS+GFP
ACCESSION   BBa_K208889
VERSION     BBa_K208889.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     misc_feature    1..1062
                     /label=BBa_F2622
     RBS             1071..1085
                     /label=RBS-1\Strong
     RBS             1078..1082
                     /label=RBS
     misc_feature    1071..1085
                     /label=BBa_B0030
     RBS             1071..1085
                     /label=BBa_B0030
     misc_feature    1092..1094
                     /label=Start
     CDS             1092..1811
                     /label=GFP Coding Region
     CDS             1092..1811
                     /label=BBa_K208000
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag attaaagagg
     1081 agaaatacta gatggctagc aaaggagaag aacttttcac tggagttgtc ccaattcttg
     1141 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     1201 atgctacata cggaaagctt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     1261 catggccaac acttgtcact actttctctt atggtgttca atgcttttcc cgttatccgg
     1321 atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac
     1381 gcactatatc tttcaaagat gacgggaact acaagacgcg tgctgaagtc aagtttgaag
     1441 gtgataccct tgttaatcgt atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     1501 ttctcggaca caaactcgag tacaactata actcacacaa tgtatacatc acggcagaca
     1561 aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacattgaa gatggatccg
     1621 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     1681 cagacaacca ttacctgtcg acacaatctg ccctttcgaa agatcccaac gaaaagcgtg
     1741 accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc
     1801 tctacaaata a
//