LOCUS BBa_K208890 3426 bp DNA linear 17-May-2024 DEFINITION RBS+GFP ACCESSION BBa_K208890 VERSION BBa_K208890.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 RBS 1071..1085 /label=RBS-1\Strong RBS 1078..1082 /label=RBS misc_feature 1071..1085 /label=BBa_B0030 RBS 1071..1085 /label=BBa_B0030 misc_feature 1092..1094 /label=Start CDS 1092..1811 /label=GFP Coding Region CDS 1092..1811 /label=BBa_K208000 RBS 1820..1832 /label=RBS-3\Weak RBS 1826..1829 /label=RBS misc_feature 1820..1832 /label=BBa_B0032 RBS 1820..1832 /label=BBa_B0032 misc_feature 1839..1840 /label=prefix misc_feature 2012..2012 /label=T misc_feature 2330..2330 /label=A CDS 1839..2588 /label=luxR misc_feature 1839..2594 /label=BBa_C0062 misc_feature 2595..2619 /label=Help:Barcodes CDS 1839..2594 /label=BBa_C0062 misc_feature 1820..2619 /label=BBa_K116616 RBS 2628..2640 /label=RBS-3\Weak RBS 2634..2637 /label=RBS misc_feature 2628..2640 /label=BBa_B0032 RBS 2628..2640 /label=BBa_B0032 misc_feature 3226..3257 /label=LVA CDS 2647..3225 /label=luxI misc_feature 2647..3264 /label=BBa_C0061 misc_feature 3265..3289 /label=Help:Barcodes CDS 2647..3264 /label=BBa_C0061 stem_loop 3309..3352 /label=stem_loop misc_feature 3298..3377 /label=BBa_B0010 terminator 3298..3377 /label=BBa_B0010 stem_loop 3393..3412 /label=T7 TE misc_feature 3419..3419 /label=stop polyA_site 3413..3426 /label=polya misc_feature 3386..3426 /label=BBa_B0012 terminator 3386..3426 /label=BBa_B0012 terminator 3298..3426 /label=BBa_B0015 misc_feature 2647..3426 /label=BBa_K116615 misc_feature 2628..3426 /label=BBa_K116625 misc_feature 1820..3426 /label=BBa_K116634 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag attaaagagg 1081 agaaatacta gatggctagc aaaggagaag aacttttcac tggagttgtc ccaattcttg 1141 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg 1201 atgctacata cggaaagctt acccttaaat ttatttgcac tactggaaaa ctacctgttc 1261 catggccaac acttgtcact actttctctt atggtgttca atgcttttcc cgttatccgg 1321 atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaac 1381 gcactatatc tttcaaagat gacgggaact acaagacgcg tgctgaagtc aagtttgaag 1441 gtgataccct tgttaatcgt atcgagttaa aaggtattga ttttaaagaa gatggaaaca 1501 ttctcggaca caaactcgag tacaactata actcacacaa tgtatacatc acggcagaca 1561 aacaaaagaa tggaatcaaa gctaacttca aaattcgcca caacattgaa gatggatccg 1621 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac 1681 cagacaacca ttacctgtcg acacaatctg ccctttcgaa agatcccaac gaaaagcgtg 1741 accacatggt ccttcttgag tttgtaactg ctgctgggat tacacatggc atggatgagc 1801 tctacaaata atactagagt cacacaggaa agtactagat gaaaaacata aatgccgacg 1861 acacatacag aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat 1921 gcttatctga tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc 1981 ctcattctat ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc 2041 aatattatga tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca 2101 atcattcacc aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa 2161 atgtaattaa agaagcgaaa acatcaggtc ttatcactgg gtttagtttc cctattcata 2221 cggctaacaa tggcttcgga atgcttagtt ttgcacattc agaaaaagac aactatatag 2281 atagtttatt tttacatgcg tgtatgaaca taccattaat tgttccttct ctagttgata 2341 attatcgaaa aataaatata gcaaataata aatcaaacaa cgatttaacc aaaagagaaa 2401 aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg ggatatttca aaaatattag 2461 gttgcagtga gcgtactgtc actttccatt taaccaatgc gcaaatgaaa ctcaatacaa 2521 caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg agcaattgat tgcccatact 2581 ttaaaaatta ataacactga tagtgctagt gtagatcact actagagtca cacaggaaag 2641 tactagatga ctataatgat aaaaaaatcg gattttttgg caattccatc ggaggagtat 2701 aaaggtattc taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt 2761 gtagaaaata accttgaatc agatgagtat gataactcaa atgcagaata tatttatgct 2821 tgtgatgata ctgaaaatgt aagtggatgc tggcgtttat tacctacaac aggtgattat 2881 atgctgaaaa gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa agatcctaat 2941 atagtcgaat taagtcgttt tgctgtaggt aaaaatagct caaagataaa taactctgct 3001 agtgaaatta caatgaaact atttgaagct atatataaac acgctgttag tcaaggtatt 3061 acagaatatg taacagtaac atcaacagca atagagcgat ttttaaagcg tattaaagtt 3121 ccttgtcatc gtattggaga caaagaaatt catgtattag gtgatactaa atcggttgta 3181 ttgtctatgc ctattaatga acagtttaaa aaagcagtct taaatgctgc aaacgacgaa 3241 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca 3301 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3361 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3421 tttata //