##gff-version 3
##sequence-region BBa_K208890 1 3426
BBa_K208890	.	CDS	1092	1811	.	+	0	ID=BBa_K208000;Name=BBa_K208000
BBa_K208890	.	start_codon	1092	1094	.	+	0	ID=annotation2062216;Name=Start;Parent=BBa_K208000
BBa_K208890	.	CDS	1092	1811	.	+	0	ID=annotation2062217;Name=GFP Coding Region;Parent=BBa_K208000
BBa_K208890	.	engineered_region	1820	3426	.	+	0	ID=BBa_K116634;Name=BBa_K116634
BBa_K208890	.	engineered_region	2628	3426	.	+	0	ID=BBa_K116625;Name=BBa_K116625;Parent=BBa_K116634
BBa_K208890	.	engineered_region	2647	3426	.	+	0	ID=BBa_K116615;Name=BBa_K116615;Parent=BBa_K116625
BBa_K208890	.	terminator	3298	3426	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K116615
BBa_K208890	.	terminator	3386	3426	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K208890	.	stem_loop	3393	3412	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K208890	.	engineered_region	3386	3426	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K208890	.	stop_codon	3419	3419	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K208890	.	polyA_site	3413	3426	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K208890	.	terminator	3298	3377	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K208890	.	stem_loop	3309	3352	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K208890	.	engineered_region	3298	3377	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K208890	.	CDS	2647	3264	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K116615
BBa_K208890	.	engineered_tag	3265	3289	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K208890	.	engineered_region	2647	3264	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K208890	.	tag	3226	3257	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K208890	.	CDS	2647	3225	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K208890	.	ribosome_entry_site	2628	2640	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116625
BBa_K208890	.	ribosome_entry_site	2628	2640	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K208890	.	engineered_region	2628	2640	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K208890	.	ribosome_entry_site	2634	2637	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K208890	.	engineered_region	1820	2619	.	+	0	ID=BBa_K116616;Name=BBa_K116616;Parent=BBa_K116634
BBa_K208890	.	CDS	1839	2594	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K116616
BBa_K208890	.	point_mutation	2330	2330	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K208890	.	engineered_region	1839	2594	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K208890	.	point_mutation	2012	2012	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K208890	.	CDS	1839	2588	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K208890	.	engineered_region	1839	1840	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K208890	.	engineered_tag	2595	2619	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K208890	.	ribosome_entry_site	1820	1832	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K116616
BBa_K208890	.	ribosome_entry_site	1820	1832	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K208890	.	engineered_region	1820	1832	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K208890	.	ribosome_entry_site	1826	1829	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K208890	.	ribosome_entry_site	1071	1085	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K208890	.	ribosome_entry_site	1078	1082	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K208890	.	engineered_region	1071	1085	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K208890	.	ribosome_entry_site	1071	1085	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K208890	.	engineered_region	1	1062	.	+	0	ID=BBa_F2622;Name=BBa_F2622
BBa_K208890	.	promoter	1008	1062	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2622
BBa_K208890	.	non_covalent_binding_site	1008	1027	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K208890	.	engineered_region	1008	1062	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K208890	.	start_codon	1060	1060	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K208890	.	promoter	1027	1032	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K208890	.	promoter	1049	1054	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K208890	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_F2622
BBa_K208890	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K208890	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K208890	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K208890	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K208890	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K208890	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2622
BBa_K208890	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K208890	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2622
BBa_K208890	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K208890	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K208890	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K208890	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K208890	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2622
BBa_K208890	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K208890	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K208890	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K208890	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K208890	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K208890	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K208890	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2622
BBa_K208890	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K208890	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K208890
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggt
ttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatggctagcaaagga
gaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtg
gagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatg
gccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgacttt
ttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtg
ctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacat
tctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaa
gctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattg
gcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcg
tgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatactag
agtcacacaggaaagtactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgta
gaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcat
ttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgac
gctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaa
acaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccc
tattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattt
ttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataata
aatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttc
aaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgc
tgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgct
agtgtagatcactactagagtcacacaggaaagtactagatgactataatgataaaaaaatcggattttttggcaattc
catcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtaga
aaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagt
ggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtg
ctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctag
tgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaaca
tcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattag
gtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacga
aaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata