LOCUS       BBa_K2089010            2366 bp    DNA     linear       27-Dec-2024
DEFINITION  Ppif1-RBS-RFP-terminator
ACCESSION   BBa_K2089010
VERSION     BBa_K2089010.1  
FEATURES             Location/Qualifiers
     promoter        1..1497
                     /label=BBa_K2089001
     misc_feature    1510..1513
                     /label=conserved
     RBS             1506..1517
                     /label=BBa_B0034
     CDS             1524..2198
                     /label=mrfp1
     misc_feature    2205..2229
                     /label=Help:Barcodes
     CDS             1524..2229
                     /label=BBa_E1010
     stem_loop       2249..2292
                     /label=stem_loop
     misc_feature    2238..2317
                     /label=BBa_B0010
     terminator      2238..2317
                     /label=BBa_B0010
     stem_loop       2333..2352
                     /label=T7 TE
     misc_feature    2359..2359
                     /label=stop
     polyA_site      2353..2366
                     /label=polya
     misc_feature    2326..2366
                     /label=BBa_B0012
     terminator      2326..2366
                     /label=BBa_B0012
     terminator      2238..2366
                     /label=BBa_B0015
ORIGIN
        1 caaaccgtta agacttgcta tctccatgac cataagactt gacaaacatg catatatatg
       61 tatcaaaccg ttaaatcaat tttgtatttg cttaataaag aagatgtaaa aagaagttta
      121 acatttatag tatttgaaaa cttatatatt agtattacta tttccttatt tttgtcaaag
      181 ttattttact atatatttcc ttaacacatc aaaatagaag gtatacttat acgtaaatga
      241 ccttaaagat ttgttaagta atggtgcatc ctcctggtcc tccatagaaa attgcactag
      301 accactcctc accgacccct gtttttgttt cgactttata acattttggg ctgtttgcga
      361 ataatcttaa atctttaact ggtggacttc taattttttc tatttcacga tctatcactt
      421 taaggccgtt cacaaaagct gcctttttga aaccttcttt tgggaaatgt ccacttccca
      481 tgagtgggct tgtactctgt ctcagtgcac tgaaaacttc tcctccccaa aacacacgac
      541 ttgctccata agctaaacct tgaacatgaa acaatgattt tggccaatac ccaattggct
      601 cgttctttat caaaaaccac cagtttcctg taatattatc ctgcttaaac caaaagcagt
      661 aactctttta gaagtagcga ttgaaattag agtttttttt tttttttttt tttatatata
      721 ggttttatgg aaattgaaac caaatgaaaa catttttaat tcttggtttt ggttcatgaa
      781 accaaatcaa aacgtaaatt tataaagttt tgcttattct ttattcgatt acacaaaaat
      841 aattttgggt tgtttctgac ttgagttata agtaaacaat agtaatcatc ggtcgatatt
      901 tttactacca tttttggtca atatatatac ctaagtgtta tgcaacatcg acttgtcaat
      961 atgagtggga tttgagaaac atggggttat atatacctga taaatgatgg cttgtaaata
     1021 gtattgttcg ccatcataag tggaagttgg tctggcaaga gttccaagtg cgagcttgct
     1081 actcacttgt acaaaccctg gacatactgt attgtagcaa cctgtcttct catgtccatc
     1141 tgcctgtttc catcataaat tccaaccaat ttagccagtg tggcttgaca tgcaatgtag
     1201 tgaactatca aactttcaat atgtttgaag aacttacggt ccaatatgta aataagccac
     1261 tattattctg atttagtttc ggggacacct gtaagatatt tatttatttt acaattttat
     1321 aggttttctt tttgaattgt tatgaaattt tgaagtaatt ttgtttttga cttactatcc
     1381 atccagctga aatgctctgt aaactgtctc taaaaccgtt ctcgacatat atacttgcaa
     1441 gactaaattg atcagggttg acaggtggat cccataaatt tatgtttccc gttgctctac
     1501 tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc
     1561 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag
     1621 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc
     1681 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg
     1741 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat
     1801 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc
     1861 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg
     1921 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg
     1981 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact
     2041 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt
     2101 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac
     2161 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt
     2221 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2281 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2341 ttcgggtggg cctttctgcg tttata
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