BBa_K2089010 1 BBa_K2089010 Ppif1-RBS-RFP-terminator 2016-10-11T11:00:00Z 2016-10-11T11:29:52Z pif1 promoter is from Arabidopsis genome DNA. This biobrick consists of a photosensitive promoter pPif1 and an rfp as a reporter. false false _2557_ 33387 33387 9 false NO. false Rouxian Zeng component2493193 1 BBa_B0015 component2493186 1 BBa_E1010 component2493183 1 BBa_B0034 component2493181 1 BBa_K2089001 annotation2493186 1 BBa_E1010 range2493186 1 1524 2229 annotation2493181 1 BBa_K2089001 range2493181 1 1 1497 annotation2493193 1 BBa_B0015 range2493193 1 2238 2366 annotation2493183 1 BBa_B0034 range2493183 1 1506 1517 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K2089001 1 BBa_K2089001 pif1 promoter from Arabidopsis 2016-10-10T11:00:00Z 2016-10-14T06:32:59Z pif promoter is from Arabidopsis genome DNA. pif promoter from Arabidopsis false false _2557_ 33387 33387 9 false No false Rouxian Zeng BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2089001_sequence 1 caaaccgttaagacttgctatctccatgaccataagacttgacaaacatgcatatatatgtatcaaaccgttaaatcaattttgtatttgcttaataaagaagatgtaaaaagaagtttaacatttatagtatttgaaaacttatatattagtattactatttccttatttttgtcaaagttattttactatatatttccttaacacatcaaaatagaaggtatacttatacgtaaatgaccttaaagatttgttaagtaatggtgcatcctcctggtcctccatagaaaattgcactagaccactcctcaccgacccctgtttttgtttcgactttataacattttgggctgtttgcgaataatcttaaatctttaactggtggacttctaattttttctatttcacgatctatcactttaaggccgttcacaaaagctgcctttttgaaaccttcttttgggaaatgtccacttcccatgagtgggcttgtactctgtctcagtgcactgaaaacttctcctccccaaaacacacgacttgctccataagctaaaccttgaacatgaaacaatgattttggccaatacccaattggctcgttctttatcaaaaaccaccagtttcctgtaatattatcctgcttaaaccaaaagcagtaactcttttagaagtagcgattgaaattagagtttttttttttttttttttttatatataggttttatggaaattgaaaccaaatgaaaacatttttaattcttggttttggttcatgaaaccaaatcaaaacgtaaatttataaagttttgcttattctttattcgattacacaaaaataattttgggttgtttctgacttgagttataagtaaacaatagtaatcatcggtcgatatttttactaccatttttggtcaatatatatacctaagtgttatgcaacatcgacttgtcaatatgagtgggatttgagaaacatggggttatatatacctgataaatgatggcttgtaaatagtattgttcgccatcataagtggaagttggtctggcaagagttccaagtgcgagcttgctactcacttgtacaaaccctggacatactgtattgtagcaacctgtcttctcatgtccatctgcctgtttccatcataaattccaaccaatttagccagtgtggcttgacatgcaatgtagtgaactatcaaactttcaatatgtttgaagaacttacggtccaatatgtaaataagccactattattctgatttagtttcggggacacctgtaagatatttatttattttacaattttataggttttctttttgaattgttatgaaattttgaagtaattttgtttttgacttactatccatccagctgaaatgctctgtaaactgtctctaaaaccgttctcgacatatatacttgcaagactaaattgatcagggttgacaggtggatcccataaatttatgtttcccgttgctc BBa_B0034_sequence 1 aaagaggagaaa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K2089010_sequence 1 caaaccgttaagacttgctatctccatgaccataagacttgacaaacatgcatatatatgtatcaaaccgttaaatcaattttgtatttgcttaataaagaagatgtaaaaagaagtttaacatttatagtatttgaaaacttatatattagtattactatttccttatttttgtcaaagttattttactatatatttccttaacacatcaaaatagaaggtatacttatacgtaaatgaccttaaagatttgttaagtaatggtgcatcctcctggtcctccatagaaaattgcactagaccactcctcaccgacccctgtttttgtttcgactttataacattttgggctgtttgcgaataatcttaaatctttaactggtggacttctaattttttctatttcacgatctatcactttaaggccgttcacaaaagctgcctttttgaaaccttcttttgggaaatgtccacttcccatgagtgggcttgtactctgtctcagtgcactgaaaacttctcctccccaaaacacacgacttgctccataagctaaaccttgaacatgaaacaatgattttggccaatacccaattggctcgttctttatcaaaaaccaccagtttcctgtaatattatcctgcttaaaccaaaagcagtaactcttttagaagtagcgattgaaattagagtttttttttttttttttttttatatataggttttatggaaattgaaaccaaatgaaaacatttttaattcttggttttggttcatgaaaccaaatcaaaacgtaaatttataaagttttgcttattctttattcgattacacaaaaataattttgggttgtttctgacttgagttataagtaaacaatagtaatcatcggtcgatatttttactaccatttttggtcaatatatatacctaagtgttatgcaacatcgacttgtcaatatgagtgggatttgagaaacatggggttatatatacctgataaatgatggcttgtaaatagtattgttcgccatcataagtggaagttggtctggcaagagttccaagtgcgagcttgctactcacttgtacaaaccctggacatactgtattgtagcaacctgtcttctcatgtccatctgcctgtttccatcataaattccaaccaatttagccagtgtggcttgacatgcaatgtagtgaactatcaaactttcaatatgtttgaagaacttacggtccaatatgtaaataagccactattattctgatttagtttcggggacacctgtaagatatttatttattttacaattttataggttttctttttgaattgttatgaaattttgaagtaattttgtttttgacttactatccatccagctgaaatgctctgtaaactgtctctaaaaccgttctcgacatatatacttgcaagactaaattgatcagggttgacaggtggatcccataaatttatgtttcccgttgctctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z