LOCUS       BBa_K2089011            2718 bp    DNA     linear       10-May-2024
DEFINITION  Pcop1-RBS-RFP-terminator
ACCESSION   BBa_K2089011
VERSION     BBa_K2089011.1  
FEATURES             Location/Qualifiers
     promoter        1..1849
                     /label=BBa_K2089002
     misc_feature    1862..1865
                     /label=conserved
     RBS             1858..1869
                     /label=BBa_B0034
     CDS             1876..2550
                     /label=mrfp1
     misc_feature    2557..2581
                     /label=Help:Barcodes
     CDS             1876..2581
                     /label=BBa_E1010
     stem_loop       2601..2644
                     /label=stem_loop
     misc_feature    2590..2669
                     /label=BBa_B0010
     terminator      2590..2669
                     /label=BBa_B0010
     stem_loop       2685..2704
                     /label=T7 TE
     misc_feature    2711..2711
                     /label=stop
     polyA_site      2705..2718
                     /label=polya
     misc_feature    2678..2718
                     /label=BBa_B0012
     terminator      2678..2718
                     /label=BBa_B0012
     terminator      2590..2718
                     /label=BBa_B0015
ORIGIN
        1 cagtacaatg tcaagcactc taagtgcatc ctgagcactc ttatctggtg tataagctcc
       61 tctctgagtg ccaagaacag tcttcaacgg tatttctgca gcataatgta tctgaacctt
      121 gtaacttctt ggcaagaaag aacgttttga tcgcttgcaa gtaccggaag aactaccacc
      181 atcagagaca ccacaatcac tataaactta acaagactgc gttagttaga agatcaacaa
      241 gatctatctt gacaccttga aacgattaaa tatcaatcca gataagaggg acataccgtt
      301 ttgaaaatga cccttccacg atgactagaa agtcaaattc gttctgtggt agaggaccaa
      361 cagtatataa agtcttctct ccatcatatg ccaacctttt accatcaaga tcagaagagt
      421 aagtcttgaa tagttggtcc ataagctttc tgcttattcc tgtcccatca acagcatcac
      481 cgttttctgt ggtgatacta acctgcaaaa aaggtaccga tatgtcaaac atctcacaca
      541 cgcacagaca ctgatgaaag aatgcagaaa agtgactaat gaatcttaca gtgtactggt
      601 aaaatactac atccggctgt ctcacagaaa cattaaagtg attggtgcac aattctattg
      661 ggttgcctgt ggttccaacc ccacgtctag ttgtgatgtc ataatctcga tggctgggct
      721 gttctggttc tatacttatg ggtgacagtg gcagagatga agatgtctcc atccttccag
      781 gggaaaataa tctgcaagag agaaaagtgg aggttggtta cagtgtgcaa aaaccagtcc
      841 aaaacacaat gctcaggaga ctaagaaaga aaagaaaaac accaaattaa gaagacaact
      901 ccaaaaggta aaaagctaaa aactttacca ccattgtcgt tctaagattt taatttctgg
      961 gcatatcctg ctaaatcgcg agaaatggta taaatttcag atgaggaaca aacttaatct
     1021 ggaaaaaaaa tggcaccttt aactgttggt cgccttcaaa attgagaaac attacacaaa
     1081 caaaaactca ttcgagtgtg tcatacagta gcataacaag actttcaaga gagaaacaac
     1141 gcagccaaaa ccatacaact atcaacagac aaatcctagc ttcatgcaaa ttctccgaaa
     1201 gagagacaaa aacaaaacct agctccgaat caacaacaat tacaacagcg atatctagtg
     1261 aaaacagcgc acctttgaaa gaattaagaa gaggatttct agggttccgg tgaatcaaag
     1321 agaggaattt atttagctca tttctcttct ccgtcacagt ctctttgggt ttcccagaca
     1381 agcaaaagaa tggagggaac gtttccagtg atgggaaaat taaaatatat ttcacttttc
     1441 agtcattttt tacagtaaca aaatttaaga actgaaataa aaatggttta ttccaaaatt
     1501 attaaaccga atccttcaaa acttattcta tttctagggt tatgctccgg ttgaatggat
     1561 tgaaattccc aacttattca tttcaaatag taagaaataa aatgaatggt tgtaagaaat
     1621 taaattagac cgtcgtggta attgtaagaa atttcattga accggactat aaattagaag
     1681 ccggataatt aaaccgtacc agcgcttgga gaaacgccag acacacgcgc gaaaagaagc
     1741 gcgtagacca gaacgtgtag gatttgtcac ctcagaaaag aaccacggtg gatcccacca
     1801 cacctcagcc tcactcctcc ttcatctccg acgtcatacc caaaccactt actagagaaa
     1861 gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa
     1921 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt
     1981 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg
     2041 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac
     2101 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt
     2161 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac
     2221 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt
     2281 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt
     2341 gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct
     2401 gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc
     2461 gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa
     2521 cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg
     2581 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     2641 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     2701 ggcctttctg cgtttata
//