LOCUS       BBa_K2089012            1682 bp    DNA     linear       21-May-2024
DEFINITION  PphyB-RBS-RFP-terminator
ACCESSION   BBa_K2089012
VERSION     BBa_K2089012.1  
FEATURES             Location/Qualifiers
     promoter        1..813
                     /label=BBa_K2089003
     misc_feature    826..829
                     /label=conserved
     RBS             822..833
                     /label=BBa_B0034
     CDS             840..1514
                     /label=mrfp1
     misc_feature    1521..1545
                     /label=Help:Barcodes
     CDS             840..1545
                     /label=BBa_E1010
     stem_loop       1565..1608
                     /label=stem_loop
     misc_feature    1554..1633
                     /label=BBa_B0010
     terminator      1554..1633
                     /label=BBa_B0010
     stem_loop       1649..1668
                     /label=T7 TE
     misc_feature    1675..1675
                     /label=stop
     polyA_site      1669..1682
                     /label=polya
     misc_feature    1642..1682
                     /label=BBa_B0012
     terminator      1642..1682
                     /label=BBa_B0012
     terminator      1554..1682
                     /label=BBa_B0015
ORIGIN
        1 gcacgtggta aattactggt ggtttaaaac tattgtacgt ggacgattct tagccaacga
       61 actagtacac tctagtgcga acaggtacat gattaaattc gtggacatcc aatcatatct
      121 cgtccaagat aagaccaaaa catatgaggt cattactcac taataaacat ttaaactttt
      181 gttttgtcaa cgaatagtgt gtttttcttt tgtcattcca atttttttct gttttctttt
      241 cactattcac ttttggtcca taatatttta tgggtatata agataatcgt ttttgtcttc
      301 atacatggta acatggatgt ttatatatgt aatagtgtta aaaagaaaaa gtggtcggtt
      361 atacttaact tattatgata gagctttgaa aacaaacaac acgagatgga gaaattagtc
      421 attcaacaaa agaaaaggac gaacgcagtg acttaacatg aaactgtgag cggcccaaaa
      481 tcatttatgt aatggaccct taacttttca tgcacacgat ttttctcatt tatatgtttt
      541 tctgctctct ttttttcctc tttatcatta ctttaattta ttttatgttc ttttttcgaa
      601 gcaccataat tgtatgcttt caccaaataa tccaaattta gaatcattaa tatgtcaaaa
      661 aagaattgca tatattcaat aaaacgtaat gctaagtagt acaatgcatg tattatacaa
      721 aatgtaatga tatagatcca acgtatatat caaagtggac caaaatatat cttatgtatt
      781 agacgagttt actatgcaaa atttatgatt ctatactaga gaaagaggag aaatactaga
      841 tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt
      901 ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta
      961 cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc
     1021 tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg
     1081 actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag
     1141 acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca
     1201 aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca
     1261 tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa
     1321 tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct
     1381 acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca
     1441 tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc
     1501 actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca
     1561 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1621 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1681 ta
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