BBa_K2091001 1 BBa_K2091001 Protocatechuate 3,4-dioxygenase Beta Subunit 2016-09-17T11:00:00Z 2016-09-19T08:55:28Z Genomic DNA of Pseudomonas putida strain KT2440. Protocatechuate 3,4-dioxygenase catalyzes the convertion of 3,4-dihydroxybenzoic acid and O2 to 3-carboxy-cis,cis-muconate. Dioxygenases are oxydoreductase enzymes. They utilize a dioxygen (O2) molecule as an oxidant. As opposed to monooxygenases, in dioxygenases reactions both atoms from O2 are incorporated into the substrate. Protocatechuate dioxygenase from P. putida has a molecular weight of 200,000 daltons and contains 4 alpha subunits of 23,000 daltons, 4 beta subunits of 26,500 daltons, and 4 ferric irons, suggesting that the enzyme is a tetramer of (𝛂𝛃Fe3+) catalytic units. false false _2559_ 30825 30825 9 false None. false Ben Vaknin BBa_K2091001_sequence 1 atgcccgcccaggacaacagccgcttcgtgatccgtgatcgcaactggcaccctaaagcccttacgcctgactacaagacctccgttgcccgctcgccgcgccaggcactggtcagcattccgcagtcgatcagcgaaaccactggtccggacttttcccatctgggcttcggcgcccacgaccatgacctgctgctgaacttcaataacggtggcctgcccattggcgagcgcatcatcgtcgccggccgtgtcgtcgaccagtacggcaagcctgtgccgaacactttggtggagatgtggcaagccaacgccggcggccgctatcgccacaagaacgatcgctacctggcgcccctggacccgaacttcggtggtgttgggcggtgtctgaccgaccgtgacggctattacagcttccgcaccatcaagccgggcccgtacccatggcgcaacggcccgaacgactggcgcccggcgcatatccacttcgccatcagcggcccatcgatcgccaccaagctgatcacccagttgtacttcgaaggtgacccgctgatcccgatgtgcccgatcgtcaagtcgatcgccaacccgcaagccgtgcagcagttgatcgccaagctcgacatgagcaacgccaacccgatggactgcctggcctaccgctttgacatcgtgctgcgcggccagcgcaagacccacttcgaaaactgctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z