BBa_K2092002
1
BBa_K2092002
PalcA, improved alcR inducible promoter from A. nidulans
2016-09-30T11:00:00Z
2016-10-02T01:52:41Z
Aspergillus nidulans, sequence found through
The alcA promoter is one of the strongest inducible promoters in Aspergillus nidulans. Its transciptional activation is dependent on the binding of its positive transcriptional regulator AlcR with various substrates that employ a hydroxyl group, for example ethanol and threonine. The native AlcA promoter consists of 3 AlcR binding sites. The number and position of the AlcR binding sites on the alcA promoter are crucial in determining its transcriptional activation strength. It has also been shown that each AlcR target in the alcA promoter contributes differently to the activation of the downstream protein expression.
This part is an improved BioBrick previously characterised by 2011 iGEM DTU-Denmark. Sequencing results showed that two Prefix/Suffix restriction sites XbaI and SpeI were absent in the original part. Hence the two missing restriction sites were added using PCR.
false
false
_2560_
31195
31195
9
false
To add the missing XbaI and SpeI restriction sites, the following two primers were designed:
alcA forward primer = 5'- CGG AAT TCG CGT CTA GAA CGT CGC TCT CCC CGA TGA C -3'
alcA reverse primer =
5'- AAC TGC AGA AAC CAA TGC ATT GGA CTA GTT TTT GAG GCG AGG TGA TAG GAT TGG -3'
false
Hui Wen Chong
annotation2485608
1
PalcA
range2485608
1
1
843
BBa_K2092002_sequence
1
acgtcgctctccccgatgacatacaggaggggtcagggcattcgagaaataccgtggatacagttgggcatttctagggctgaatgggaaggagagagttttgaaataggcgttccgttctgcttagggtatttgggaacaatcaatgttcaatgtacatttaatccacgattttataaaacgtcatcctttgccctcccttcttatttgccaataccaaaaatcttactccagtggttcggtaatcgcagagttaaatctgggctcggtggcagatctgcgatgctccataaccgttcagatgttgattggaactgggtggggtagacagctcgaagaccgagtgaacgtatacctaagacactttgacacggccggaacactgtaagtcccttcgtatttctccgcctgtgtggagctaccatccaataacccccagctgaaaagctgattgtgatagttcccacttgtccgtccgcatcggcatccgcagctcgggatagttccgacctaggattggatgcatgcggaaccgcacgagggcggggcggaaattgacacaccactcctctccacgcaccgttcaagaggtacgcgtatagagccgtatagagcagagacggagcactttctggtactgtccgcacgggatgtccgcacggagagccacaaacgagcggggccccgtacgtgctctcctaccccaggatcgcatccccgcatagctgaacatctatataaagacccccaaggttctcagtctcaccaacatcatcaaccaacaatcaacagttctctactcagttaattagaactcttccaatcctatcacctcgcctcaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z