BBa_J34801 1 BBa_J34801 ribosome binding site 2006-10-25T11:00:00Z 2015-08-31T04:08:46Z a Released HQ 2013 a false false _62_ 0 1085 62 In stock false a false Alexandra Choutko BBa_K2092001 1 BBa_K2092001 alcR, ethanol-activated transcription factor from A. nidulans 2016-09-22T11:00:00Z 2016-10-09T04:31:21Z Aspergillus nidulans This part encodes for AlcR protein, which is a transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. Upstream of the start codon an RBS is already placed. false false _2560_ 31195 30886 9 false - false Sathya Darmalinggam annotation2484557 1 Start range2484557 1 1 3 annotation2484558 1 stop range2484558 1 2593 2595 annotation2484559 1 alcR range2484559 1 4 2592 BBa_K2092005 1 BBa_K2092005 alcR, ethanol-activated transcription factor from A. nidulans (incl. RBS) 2016-10-01T11:00:00Z 2016-10-09T04:34:33Z Aspergillus nidulans, sequence found through This part encodes for the <i>alcR</i> gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus <i>Aspergillus nidulans (A.nidulans)</i>. It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA <a href="http://parts.igem.org/Part:BBa_K2092002">(PalcA)</a> and aldA promoters. AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1]. Reference: [1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. <i>Gene</i>, 73(2), 385???396. true false _2560_ 31195 31195 9 false - Codon optimized the sequence retrieved from the NCBI database. - Calculated the RBS strength by using the online tool SalisLab RBS Calculator. false Hui Wen Chong component2485616 1 BBa_J34801 component2485620 1 BBa_K2092001 annotation2485620 1 BBa_K2092001 range2485620 1 19 2613 annotation2485616 1 BBa_J34801 range2485616 1 1 12 BBa_K2092001_sequence 1 atgtgttggaatccggcatgtagccgtccgcatagtccggattttgatagcatggcagatagcattcagaccgcacgtttttggaccagcgataccatttataccgatattaccgttgatatcctgctgcataccgcctggcagattcgtgcagatgcacgtattattgcagcaattccggttgcacgtgccagtgatgcagttatgcctcgtaatgaagcaaatgaaaatggttgggttagctgtagcaattgcaaacgctggaataaagattgcacctttaattggctgagcagccagcgtagcaaagcaaaaggtgcagcaccgcgtgcacgtaccaaaaaagcacgtaccgcaaccaccaccagtgaaccgagcaccagcgcagccaccattccgacaccggaaagcgataatcatgatgcaccgcctgtgattaatagccatgatgccctgccgagctggacccagggtctgctgagtcatccgggtgacctgtttgattttagccatagcgccattccggcaaatgccgaagatgcagcaaatgttcagtcagatgcaccgtttccgtgggatctggcgattccgggtgattttagtatgggtcagcagctggaaaaaccgctgagtccgctgagctttcaggcagttctgctgcctccgcatagcccgaataccgatgatctgattcgtgaactggaagaacagaccaccgatccggatagcgttaccgataccaatagcgttcagcaggttgcacaggatggtagcctgtggtcagatcgtcagtcaccgctgctgccggaaaatagcctgtgtatggcaagcgatagcaccgcacgtcgttatgcacgtagcaccatgaccaaaaatctgatgcgtatctatcatgacagcatggaaaatgcactgagctgttggctgaccgaacataattgtccgtatagcgatcagattagttatctgcctccgaaacagcgtgcagaatggggtccgaattggagcaatcgtatgtgtattcgtgtttgtcgtctggatcgtgttagcaccagcctgcgtggtcgtgccctgagcgcagaagaagataaagcagcagcacgtgcactgcatctggcaattgttgcatttgcaagccagtggacccagcatgcacagcgtggtgcaggcctgaatgttccggcagatattgcagccgatgaacgtagcattcgtcgtaatgcatggaatgaagcacgtcatgcactgcaacataccaccggtattccgagctttcgtgttatttttgccaacatcatttttagcctgacccagagcgttctggatgatgatgaacagcatggtatgggtgcacgtctggataaactgctggaaaatgatggtgcaccggtttttctggaaaccgcaaatcgtcagctgtatacctttcgtcataaatttgcacgtatgcagcgtcgtggtaaagcatttaatcgtctgcctggtggtagcgttgcaagcacctttgcaggtatttttgaaaccccgaccccgagcagcgaaagtccgcagctggatccggttgttgcaagcgaagaacatcgtagcaccctgagcctgatgttttggctgggtattatgtttgataccctgagtgcagcaatgtatcagcgtccgctggttgttagtgatgaagatagccagattagcagcgcaagccctccgcgtcgcggtgcagaaacaccgattaatctggattgttgggaaccgcctcgtcaggttccgagcaatcaagaaaaaagtgatgtttggggtgacctgttcctgcgtaccagcgatagcctgccggatcatgaaagccatacccagatttcacagcctgccgcacgttggccgtgtacctatgaacaggcagcagcagccctgagcagcgccacaccggttaaagtgctgctgtatcgtcgtgttacccagctgcaaaccctgctgtaccgtggtgcaagtccggcacgcctggaagcagccattcagcgtaccctgtatgtttataatcattggaccgcaaaataccagccgtttatgcaggattgtgttgcaaatcatgaactgctgccgagccgtattcagagctggtatgttattctggatggtcattggcacctggcagcaatgctgctggcagatgttctggaaagcattgatcgtgatagctatagcgatatcaaccatattgatctggttaccaaactgcgcctggataatgccctggcagtttcagccctggcacgtagctcactgcgtggccaagaactggatcctggtaaagccagcccgatgtatcgtcattttcatgatagtctgaccgaagttgcatttctggttgaaccgtggaccgttgttctgattcatagctttgcaaaagcagcctatattctgctggattgtctggatctggatggccagggtaacgcactggcaggttatctgcaactgcgtcagaattgtaattattgcattcgtgcgctgcaatttctgggtcgtaaaagcgatatggcagcactggttgccaaagatctggaacgtggtctgaacggtaaagttgatagctttctgtaa BBa_J34801_sequence 1 aaagaggagaaa BBa_K2092005_sequence 1 aaagaggagaaatactagatgtgttggaatccggcatgtagccgtccgcatagtccggattttgatagcatggcagatagcattcagaccgcacgtttttggaccagcgataccatttataccgatattaccgttgatatcctgctgcataccgcctggcagattcgtgcagatgcacgtattattgcagcaattccggttgcacgtgccagtgatgcagttatgcctcgtaatgaagcaaatgaaaatggttgggttagctgtagcaattgcaaacgctggaataaagattgcacctttaattggctgagcagccagcgtagcaaagcaaaaggtgcagcaccgcgtgcacgtaccaaaaaagcacgtaccgcaaccaccaccagtgaaccgagcaccagcgcagccaccattccgacaccggaaagcgataatcatgatgcaccgcctgtgattaatagccatgatgccctgccgagctggacccagggtctgctgagtcatccgggtgacctgtttgattttagccatagcgccattccggcaaatgccgaagatgcagcaaatgttcagtcagatgcaccgtttccgtgggatctggcgattccgggtgattttagtatgggtcagcagctggaaaaaccgctgagtccgctgagctttcaggcagttctgctgcctccgcatagcccgaataccgatgatctgattcgtgaactggaagaacagaccaccgatccggatagcgttaccgataccaatagcgttcagcaggttgcacaggatggtagcctgtggtcagatcgtcagtcaccgctgctgccggaaaatagcctgtgtatggcaagcgatagcaccgcacgtcgttatgcacgtagcaccatgaccaaaaatctgatgcgtatctatcatgacagcatggaaaatgcactgagctgttggctgaccgaacataattgtccgtatagcgatcagattagttatctgcctccgaaacagcgtgcagaatggggtccgaattggagcaatcgtatgtgtattcgtgtttgtcgtctggatcgtgttagcaccagcctgcgtggtcgtgccctgagcgcagaagaagataaagcagcagcacgtgcactgcatctggcaattgttgcatttgcaagccagtggacccagcatgcacagcgtggtgcaggcctgaatgttccggcagatattgcagccgatgaacgtagcattcgtcgtaatgcatggaatgaagcacgtcatgcactgcaacataccaccggtattccgagctttcgtgttatttttgccaacatcatttttagcctgacccagagcgttctggatgatgatgaacagcatggtatgggtgcacgtctggataaactgctggaaaatgatggtgcaccggtttttctggaaaccgcaaatcgtcagctgtatacctttcgtcataaatttgcacgtatgcagcgtcgtggtaaagcatttaatcgtctgcctggtggtagcgttgcaagcacctttgcaggtatttttgaaaccccgaccccgagcagcgaaagtccgcagctggatccggttgttgcaagcgaagaacatcgtagcaccctgagcctgatgttttggctgggtattatgtttgataccctgagtgcagcaatgtatcagcgtccgctggttgttagtgatgaagatagccagattagcagcgcaagccctccgcgtcgcggtgcagaaacaccgattaatctggattgttgggaaccgcctcgtcaggttccgagcaatcaagaaaaaagtgatgtttggggtgacctgttcctgcgtaccagcgatagcctgccggatcatgaaagccatacccagatttcacagcctgccgcacgttggccgtgtacctatgaacaggcagcagcagccctgagcagcgccacaccggttaaagtgctgctgtatcgtcgtgttacccagctgcaaaccctgctgtaccgtggtgcaagtccggcacgcctggaagcagccattcagcgtaccctgtatgtttataatcattggaccgcaaaataccagccgtttatgcaggattgtgttgcaaatcatgaactgctgccgagccgtattcagagctggtatgttattctggatggtcattggcacctggcagcaatgctgctggcagatgttctggaaagcattgatcgtgatagctatagcgatatcaaccatattgatctggttaccaaactgcgcctggataatgccctggcagtttcagccctggcacgtagctcactgcgtggccaagaactggatcctggtaaagccagcccgatgtatcgtcattttcatgatagtctgaccgaagttgcatttctggttgaaccgtggaccgttgttctgattcatagctttgcaaaagcagcctatattctgctggattgtctggatctggatggccagggtaacgcactggcaggttatctgcaactgcgtcagaattgtaattattgcattcgtgcgctgcaatttctgggtcgtaaaagcgatatggcagcactggttgccaaagatctggaacgtggtctgaacggtaaagttgatagctttctgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z