BBa_K2092005
1
BBa_K2092005
alcR, ethanol-activated transcription factor from A. nidulans (incl. RBS)
2016-10-01T11:00:00Z
2016-10-09T04:34:33Z
Aspergillus nidulans, sequence found through
This part encodes for the <i>alcR</i> gene, which is a positive regulatory gene in the ethanol regulon of filamentous fungus <i>Aspergillus nidulans (A.nidulans)</i>. It encodes the AlcR protein, an ethanol-activated transcription factor which would bind to its target promoters, including the alcA <a href="http://parts.igem.org/Part:BBa_K2092002">(PalcA)</a> and aldA promoters.
AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [1].
Reference:
[1] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. <i>Gene</i>, 73(2), 385???396.
true
false
_2560_
31195
31195
9
false
- Codon optimized the sequence retrieved from the NCBI database.
- Calculated the RBS strength by using the online tool SalisLab RBS Calculator.
false
Hui Wen Chong
component2485620
1
BBa_K2092001
component2485616
1
BBa_J34801
annotation2485620
1
BBa_K2092001
range2485620
1
19
2613
annotation2485616
1
BBa_J34801
range2485616
1
1
12
BBa_J34801
1
BBa_J34801
ribosome binding site
2006-10-25T11:00:00Z
2015-08-31T04:08:46Z
a
Released HQ 2013
a
false
false
_62_
0
1085
62
In stock
false
a
false
Alexandra Choutko
BBa_K2092001
1
BBa_K2092001
alcR, ethanol-activated transcription factor from A. nidulans
2016-09-22T11:00:00Z
2016-10-09T04:31:21Z
Aspergillus nidulans
This part encodes for AlcR protein, which is a transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. Upstream of the start codon an RBS is already placed.
false
false
_2560_
31195
30886
9
false
-
false
Sathya Darmalinggam
annotation2484557
1
Start
range2484557
1
1
3
annotation2484558
1
stop
range2484558
1
2593
2595
annotation2484559
1
alcR
range2484559
1
4
2592
BBa_K2092001_sequence
1
atgtgttggaatccggcatgtagccgtccgcatagtccggattttgatagcatggcagatagcattcagaccgcacgtttttggaccagcgataccatttataccgatattaccgttgatatcctgctgcataccgcctggcagattcgtgcagatgcacgtattattgcagcaattccggttgcacgtgccagtgatgcagttatgcctcgtaatgaagcaaatgaaaatggttgggttagctgtagcaattgcaaacgctggaataaagattgcacctttaattggctgagcagccagcgtagcaaagcaaaaggtgcagcaccgcgtgcacgtaccaaaaaagcacgtaccgcaaccaccaccagtgaaccgagcaccagcgcagccaccattccgacaccggaaagcgataatcatgatgcaccgcctgtgattaatagccatgatgccctgccgagctggacccagggtctgctgagtcatccgggtgacctgtttgattttagccatagcgccattccggcaaatgccgaagatgcagcaaatgttcagtcagatgcaccgtttccgtgggatctggcgattccgggtgattttagtatgggtcagcagctggaaaaaccgctgagtccgctgagctttcaggcagttctgctgcctccgcatagcccgaataccgatgatctgattcgtgaactggaagaacagaccaccgatccggatagcgttaccgataccaatagcgttcagcaggttgcacaggatggtagcctgtggtcagatcgtcagtcaccgctgctgccggaaaatagcctgtgtatggcaagcgatagcaccgcacgtcgttatgcacgtagcaccatgaccaaaaatctgatgcgtatctatcatgacagcatggaaaatgcactgagctgttggctgaccgaacataattgtccgtatagcgatcagattagttatctgcctccgaaacagcgtgcagaatggggtccgaattggagcaatcgtatgtgtattcgtgtttgtcgtctggatcgtgttagcaccagcctgcgtggtcgtgccctgagcgcagaagaagataaagcagcagcacgtgcactgcatctggcaattgttgcatttgcaagccagtggacccagcatgcacagcgtggtgcaggcctgaatgttccggcagatattgcagccgatgaacgtagcattcgtcgtaatgcatggaatgaagcacgtcatgcactgcaacataccaccggtattccgagctttcgtgttatttttgccaacatcatttttagcctgacccagagcgttctggatgatgatgaacagcatggtatgggtgcacgtctggataaactgctggaaaatgatggtgcaccggtttttctggaaaccgcaaatcgtcagctgtatacctttcgtcataaatttgcacgtatgcagcgtcgtggtaaagcatttaatcgtctgcctggtggtagcgttgcaagcacctttgcaggtatttttgaaaccccgaccccgagcagcgaaagtccgcagctggatccggttgttgcaagcgaagaacatcgtagcaccctgagcctgatgttttggctgggtattatgtttgataccctgagtgcagcaatgtatcagcgtccgctggttgttagtgatgaagatagccagattagcagcgcaagccctccgcgtcgcggtgcagaaacaccgattaatctggattgttgggaaccgcctcgtcaggttccgagcaatcaagaaaaaagtgatgtttggggtgacctgttcctgcgtaccagcgatagcctgccggatcatgaaagccatacccagatttcacagcctgccgcacgttggccgtgtacctatgaacaggcagcagcagccctgagcagcgccacaccggttaaagtgctgctgtatcgtcgtgttacccagctgcaaaccctgctgtaccgtggtgcaagtccggcacgcctggaagcagccattcagcgtaccctgtatgtttataatcattggaccgcaaaataccagccgtttatgcaggattgtgttgcaaatcatgaactgctgccgagccgtattcagagctggtatgttattctggatggtcattggcacctggcagcaatgctgctggcagatgttctggaaagcattgatcgtgatagctatagcgatatcaaccatattgatctggttaccaaactgcgcctggataatgccctggcagtttcagccctggcacgtagctcactgcgtggccaagaactggatcctggtaaagccagcccgatgtatcgtcattttcatgatagtctgaccgaagttgcatttctggttgaaccgtggaccgttgttctgattcatagctttgcaaaagcagcctatattctgctggattgtctggatctggatggccagggtaacgcactggcaggttatctgcaactgcgtcagaattgtaattattgcattcgtgcgctgcaatttctgggtcgtaaaagcgatatggcagcactggttgccaaagatctggaacgtggtctgaacggtaaagttgatagctttctgtaa
BBa_J34801_sequence
1
aaagaggagaaa
BBa_K2092005_sequence
1
aaagaggagaaatactagatgtgttggaatccggcatgtagccgtccgcatagtccggattttgatagcatggcagatagcattcagaccgcacgtttttggaccagcgataccatttataccgatattaccgttgatatcctgctgcataccgcctggcagattcgtgcagatgcacgtattattgcagcaattccggttgcacgtgccagtgatgcagttatgcctcgtaatgaagcaaatgaaaatggttgggttagctgtagcaattgcaaacgctggaataaagattgcacctttaattggctgagcagccagcgtagcaaagcaaaaggtgcagcaccgcgtgcacgtaccaaaaaagcacgtaccgcaaccaccaccagtgaaccgagcaccagcgcagccaccattccgacaccggaaagcgataatcatgatgcaccgcctgtgattaatagccatgatgccctgccgagctggacccagggtctgctgagtcatccgggtgacctgtttgattttagccatagcgccattccggcaaatgccgaagatgcagcaaatgttcagtcagatgcaccgtttccgtgggatctggcgattccgggtgattttagtatgggtcagcagctggaaaaaccgctgagtccgctgagctttcaggcagttctgctgcctccgcatagcccgaataccgatgatctgattcgtgaactggaagaacagaccaccgatccggatagcgttaccgataccaatagcgttcagcaggttgcacaggatggtagcctgtggtcagatcgtcagtcaccgctgctgccggaaaatagcctgtgtatggcaagcgatagcaccgcacgtcgttatgcacgtagcaccatgaccaaaaatctgatgcgtatctatcatgacagcatggaaaatgcactgagctgttggctgaccgaacataattgtccgtatagcgatcagattagttatctgcctccgaaacagcgtgcagaatggggtccgaattggagcaatcgtatgtgtattcgtgtttgtcgtctggatcgtgttagcaccagcctgcgtggtcgtgccctgagcgcagaagaagataaagcagcagcacgtgcactgcatctggcaattgttgcatttgcaagccagtggacccagcatgcacagcgtggtgcaggcctgaatgttccggcagatattgcagccgatgaacgtagcattcgtcgtaatgcatggaatgaagcacgtcatgcactgcaacataccaccggtattccgagctttcgtgttatttttgccaacatcatttttagcctgacccagagcgttctggatgatgatgaacagcatggtatgggtgcacgtctggataaactgctggaaaatgatggtgcaccggtttttctggaaaccgcaaatcgtcagctgtatacctttcgtcataaatttgcacgtatgcagcgtcgtggtaaagcatttaatcgtctgcctggtggtagcgttgcaagcacctttgcaggtatttttgaaaccccgaccccgagcagcgaaagtccgcagctggatccggttgttgcaagcgaagaacatcgtagcaccctgagcctgatgttttggctgggtattatgtttgataccctgagtgcagcaatgtatcagcgtccgctggttgttagtgatgaagatagccagattagcagcgcaagccctccgcgtcgcggtgcagaaacaccgattaatctggattgttgggaaccgcctcgtcaggttccgagcaatcaagaaaaaagtgatgtttggggtgacctgttcctgcgtaccagcgatagcctgccggatcatgaaagccatacccagatttcacagcctgccgcacgttggccgtgtacctatgaacaggcagcagcagccctgagcagcgccacaccggttaaagtgctgctgtatcgtcgtgttacccagctgcaaaccctgctgtaccgtggtgcaagtccggcacgcctggaagcagccattcagcgtaccctgtatgtttataatcattggaccgcaaaataccagccgtttatgcaggattgtgttgcaaatcatgaactgctgccgagccgtattcagagctggtatgttattctggatggtcattggcacctggcagcaatgctgctggcagatgttctggaaagcattgatcgtgatagctatagcgatatcaaccatattgatctggttaccaaactgcgcctggataatgccctggcagtttcagccctggcacgtagctcactgcgtggccaagaactggatcctggtaaagccagcccgatgtatcgtcattttcatgatagtctgaccgaagttgcatttctggttgaaccgtggaccgttgttctgattcatagctttgcaaaagcagcctatattctgctggattgtctggatctggatggccagggtaacgcactggcaggttatctgcaactgcgtcagaattgtaattattgcattcgtgcgctgcaatttctgggtcgtaaaagcgatatggcagcactggttgccaaagatctggaacgtggtctgaacggtaaagttgatagctttctgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z