BBa_K2100031 1 BBa_K2100031 pEXPR pPRE4:eYFP 2016-10-16T11:00:00Z 2016-10-17T04:13:36Z This is a synthetic promoter and a gene from a jellyfish's genome. description here. false false _2568_ 31606 31606 9 false This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts. false Kathleen Brandes component2515456 1 BBa_K2100009 component2515457 1 BBa_K2100005 annotation2515457 1 BBa_K2100005 range2515457 1 295 1031 annotation2515456 1 BBa_K2100009 range2515456 1 1 286 BBa_K2100005 1 BBa_K2100005 pENTR eYFP 2016-10-09T11:00:00Z 2016-10-10T09:55:55Z This is a gene derived from a jellyfish's genome. eYFP gene description here. false false _2568_ 31606 31606 9 false This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. This can be cascade with a promoter (flanked by L4, R1 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts. false Kathleen Brandes BBa_K2100009 1 BBa_K2100009 pENTR pPRE4 2016-10-09T11:00:00Z 2016-10-10T09:57:26Z This is a synthetic promoter from IDT. description here. false false _2568_ 31606 31606 9 false This basic part entry vector is flanked by L4 and R1 sites, which are used to denote a promoter. This can be cascade with a gene (flanked by L1, L2 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts. false Kathleen Brandes annotation2490385 1 PRE site range2490385 1 121 136 annotation2490386 1 PRE site range2490386 1 86 101 annotation2490387 1 PRE site range2490387 1 157 172 annotation2490384 1 PRE site range2490384 1 50 65 annotation2490376 1 minCMV range2490376 1 184 241 BBa_K2100009_sequence 1 gctccgaattcgaggctaggtctcaaggtgtatgtcgagtttactaacgcgggacatggtgttctgtatgtcgagtttactttgtaggtacatggtgttctgtaagtcgagtttattttgcaggacatggtgttctgtatgtcgagtttactttgacggtacgtggtgttctgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgacgctttgagaccagacctcgaattcgac BBa_K2100005_sequence 1 atccaccggtcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa BBa_K2100031_sequence 1 gctccgaattcgaggctaggtctcaaggtgtatgtcgagtttactaacgcgggacatggtgttctgtatgtcgagtttactttgtaggtacatggtgttctgtaagtcgagtttattttgcaggacatggtgttctgtatgtcgagtttactttgacggtacgtggtgttctgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgacgctttgagaccagacctcgaattcgactactagagatccaccggtcgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z