BBa_K2100004 1 BBa_K2100004 pENTR pTRE 2016-10-09T11:00:00Z 2016-10-10T09:55:39Z This is a synthetic promoter. description here! false false _2568_ 31606 31606 9 false This part was created using scarless golden gate assembly. This basic part is flanked by L1 and L2 sites and can be easily cloned into an entry vector using an LR reaction. A promoter can be easily inserted in front of this part in a one pot LR reaction with a promoter flanked by L4 and R1 sites cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts. false Kathleen Brandes annotation2490244 1 tetO binding site range2490244 1 222 236 annotation2490245 1 tetO binding site range2490245 1 44 58 annotation2490227 1 tetO binding site range2490227 1 77 91 annotation2490247 1 tetO binding site range2490247 1 187 201 annotation2490246 1 tetO binding site range2490246 1 116 130 annotation2490243 1 tetO binding site range2490243 1 150 164 annotation2490217 1 minCMV range2490217 1 253 312 BBa_K2100024 1 BBa_K2100024 pENTR pTAL14 2016-10-13T11:00:00Z 2016-10-14T05:24:59Z This is from the mammalian genome. description here. false false _2568_ 31606 31606 9 false This basic part entry vector is flanked by L4 and R1 sites, which are used to denote a promoter. This can be cascade with a gene (flanked by L1, L2 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts. false Kathleen Brandes BBa_K2100037 1 BBa_K2100037 pEXPR TRE - TALER14 2016-10-16T11:00:00Z 2016-10-17T05:15:01Z Comes from some genomic sequence. This part is the result of a gateway combining a tet response element promoter (TRE) and the repressor TALER14 (Tal14). The repressor can bind to sites on the Tal14 promoter. false false _2568_ 32888 32888 9 false None false Wangui Mbuguiro component2515678 1 BBa_K2100024 component2515677 1 BBa_K2100004 annotation2515677 1 BBa_K2100004 range2515677 1 1 330 annotation2515678 1 BBa_K2100024 range2515678 1 339 682 BBa_K2100024_sequence 1 gctccgaattcacctgctgacaggtgatctcatgtgattacgccaagctacgggcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagttctgtcctccgagcggagactctagatacccctctccgcttctgaattctaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctcgagtacccctctccgcttcttacccctctccgcttcttacccctctccgcttctggatccaattcgcttggtctcggcttcagtgcaggtgagctt BBa_K2100004_sequence 1 gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgac BBa_K2100037_sequence 1 gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgactactagaggctccgaattcacctgctgacaggtgatctcatgtgattacgccaagctacgggcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagttctgtcctccgagcggagactctagatacccctctccgcttctgaattctaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctcgagtacccctctccgcttcttacccctctccgcttcttacccctctccgcttctggatccaattcgcttggtctcggcttcagtgcaggtgagctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z