BBa_K2100004
1
BBa_K2100004
pENTR pTRE
2016-10-09T11:00:00Z
2016-10-10T09:55:39Z
This is a synthetic promoter.
description here!
false
false
_2568_
31606
31606
9
false
This part was created using scarless golden gate assembly. This basic part is flanked by L1 and L2 sites and can be easily cloned into an entry vector using an LR reaction. A promoter can be easily inserted in front of this part in a one pot LR reaction with a promoter flanked by L4 and R1 sites cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
false
Kathleen Brandes
annotation2490244
1
tetO binding site
range2490244
1
222
236
annotation2490245
1
tetO binding site
range2490245
1
44
58
annotation2490227
1
tetO binding site
range2490227
1
77
91
annotation2490247
1
tetO binding site
range2490247
1
187
201
annotation2490246
1
tetO binding site
range2490246
1
116
130
annotation2490243
1
tetO binding site
range2490243
1
150
164
annotation2490217
1
minCMV
range2490217
1
253
312
BBa_K2100024
1
BBa_K2100024
pENTR pTAL14
2016-10-13T11:00:00Z
2016-10-14T05:24:59Z
This is from the mammalian genome.
description here.
false
false
_2568_
31606
31606
9
false
This basic part entry vector is flanked by L4 and R1 sites, which are used to denote a promoter. This can be cascade with a gene (flanked by L1, L2 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
false
Kathleen Brandes
BBa_K2100037
1
BBa_K2100037
pEXPR TRE - TALER14
2016-10-16T11:00:00Z
2016-10-17T05:15:01Z
Comes from some genomic sequence.
This part is the result of a gateway combining a tet response element promoter (TRE) and the repressor TALER14 (Tal14). The repressor can bind to sites on the Tal14 promoter.
false
false
_2568_
32888
32888
9
false
None
false
Wangui Mbuguiro
component2515678
1
BBa_K2100024
component2515677
1
BBa_K2100004
annotation2515677
1
BBa_K2100004
range2515677
1
1
330
annotation2515678
1
BBa_K2100024
range2515678
1
339
682
BBa_K2100024_sequence
1
gctccgaattcacctgctgacaggtgatctcatgtgattacgccaagctacgggcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagttctgtcctccgagcggagactctagatacccctctccgcttctgaattctaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctcgagtacccctctccgcttcttacccctctccgcttcttacccctctccgcttctggatccaattcgcttggtctcggcttcagtgcaggtgagctt
BBa_K2100004_sequence
1
gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgac
BBa_K2100037_sequence
1
gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgactactagaggctccgaattcacctgctgacaggtgatctcatgtgattacgccaagctacgggcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagtactgtcctccgagcggagttctgtcctccgagcggagactctagatacccctctccgcttctgaattctaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctcgagtacccctctccgcttcttacccctctccgcttcttacccctctccgcttctggatccaattcgcttggtctcggcttcagtgcaggtgagctt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z