LOCUS       BBa_K210005             1836 bp    DNA     linear       20-Dec-2024
DEFINITION  LacI generator yeast-optimized
ACCESSION   BBa_K210005
VERSION     BBa_K210005.1  
FEATURES             Location/Qualifiers
     promoter        1..403
                     /label=pTEF2(BBa_K165037)
     RBS             414..425
                     /label=kozak
     CDS             420..1544
                     /label=LacI
     polyA_site      1584..1835
                     /label=NLS+terminator(BBa_K165018)
ORIGIN
        1 attacccata aggttgtttg tgacggcgtc gtacaagaga acgtgggaac tttttaggct
       61 caccaaaaaa gaaagaaaaa atacgagttg ctgacagaag cctcaagaaa aaaaaaattc
      121 ttcttcgact atgctggagg cagagatgat cgagccggta gttaactata tatagctaaa
      181 ttggttccat caccttcttt tctggtgtcg ctccttctag tgctatttct ggcttttcct
      241 attttttttt ttccattttt ctttctctct ttctaatata taaattctct tgcattttct
      301 atttttctct ctatctattc tacttgttta ttcccttcaa ggtttttttt taaggagtac
      361 ttgtttttag aatatacggt caacgaacta taattaacta aactactaga ggcaaaaaaa
      421 tgtctgtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
      481 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
      541 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
      601 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
      661 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
      721 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
      781 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
      841 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
      901 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
      961 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     1021 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     1081 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     1141 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     1201 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     1261 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     1321 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     1381 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     1441 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1501 aaagcgggca ggctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt
     1561 gctagtgtag atctctacta gagcccaaga aaaagcgcaa ggtaactaga taataagcga
     1621 atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca
     1681 aattttaaag tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc
     1741 tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta
     1801 ccggcatgcc gagcaaatgc ctgcaaatcg ctccct
//