LOCUS BBa_K2100051 11030 bp DNA linear 29-Nov-2024 DEFINITION Sensor 20A ACCESSION BBa_K2100051 VERSION BBa_K2100051.1 FEATURES Location/Qualifiers misc_feature 5..92 /label=miRNA-20A target site x4 CDS 10268..10972 /label=mKate promoter 9030..10203 /label=hEF1A promoter 1461..2634 /label=hEF1A CDS 2690..3409 /label=EBFP ORIGIN 1 gcttctttaa gtgctcataa tgcagtcttt aagtgctcat aatgcagtct ttaagtgctc 61 ataatgcagt ctttaagtgc tcataatgca gtcaaccaga agagacctgt gcttcactcc 121 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa 181 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga 241 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt 301 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga 361 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg 421 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag 481 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt 541 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact 601 actcccagtc atagctgtcc ctcttctctt atggagatcc cgagcggtgc gtttttatgc 661 ttgtagtatt gtataatgtt tttaagatcc aagcttctga cctcttctct tcctcccaca 721 gggcctcgag acaattgcat cgatggtacc agtgaaagtc tgcaatgaat tcgagttggc 781 gcgcctgtca ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt 841 gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc 901 cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc 961 ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc 1021 cctgggggct ttgggagggg gctgtccccg tgagctccca ggcgcgcctg tcattctaaa 1081 tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca ggctcaaaga 1141 gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc 1201 cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc 1261 ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg gctttggggg 1321 ggggctgtcc ccgtgagctc ccaggcgcgc caactcggta ccgtatcgat gtcgacgtta 1381 acgctagtga tatcaacttt gtatagaaaa gttggctccg aattcgccct tgacagcaga 1441 gatccagttt ggttaattaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc 1501 cacagtcccc gagaagttgt ggggaggggt cggcaattga accggtgcct agagaaggtg 1561 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg 1621 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc 1681 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta 1741 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga 1801 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc 1861 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca 1921 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc 1981 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac 2041 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg 2101 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc 2161 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc 2221 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc 2281 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac 2341 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg 2401 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg 2461 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag 2521 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg 2581 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg 2641 agaagggcga attcgaccca agtttgtaca aaaaagcagg ctcgccacca tggtgagcaa 2701 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 2761 cggccacaag ttcagcgtga ggggcgaggg cgagggcgat gccaccaacg gcaagctgac 2821 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 2881 cctgagccac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt 2941 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 3001 cggcacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctag tgaaccgcat 3061 cgagctgaag ggcgtcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 3121 caacttcaac agccacaaca tctatatcat ggccgtcaag cagaagaacg gcatcaaggt 3181 gaacttcaag atccgccaca acgtggagga cggcagcgtg cagctcgccg accactacca 3241 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacagccact acctgagcac 3301 ccagtccgtg ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 3361 ccgcaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat accacccagc 3421 tttcttgtac aaagtggtac gcgtgaattc actcctcagg tgcaggctgc ctatcagaag 3481 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct 3541 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg 3601 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca 3661 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca 3721 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg 3781 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt 3841 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat 3901 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt 3961 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt 4021 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 4081 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 4141 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc gacagtacta agcttggtct 4201 aatacccaat ctctcgtctt atccagatgt tttatacgcc tctggcccgt gtctcaaaat 4261 ctctgatgtt acattgcaca agataaaata atatcatcat gaacaataaa actgtctgct 4321 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg 4381 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc 4441 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt 4501 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac 4561 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat 4621 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat 4681 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg 4741 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa 4801 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg 4861 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc 4921 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt 4981 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg 5041 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt 5101 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca 5161 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca 5221 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagaggta 5281 tcactagtat tacagaggta agttataaca gtcgcctaac ccaattgagg ggataacgca 5341 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 5401 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 5461 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 5521 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 5581 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 5641 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 5701 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 5761 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 5821 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 5881 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 5941 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 6001 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 6061 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 6121 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 6181 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 6241 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 6301 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccgga 6361 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 6421 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 6481 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 6541 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 6601 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 6661 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 6721 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 6781 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 6841 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 6901 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 6961 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 7021 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 7081 agcggataca tatttgaatg tagctccgaa tttctcgagg gcttgtcgac gacggcggtc 7141 tccgtcgtca ggatcatgaa ttcgacccgc ctgccaccat gaccgagtac aagcccacgg 7201 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg 7261 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg 7321 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 7381 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 7441 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 7501 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 7561 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 7621 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacatccgcg ccccgcaacc 7681 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 7741 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgata cgcgtgaatt cactcctcag 7801 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac 7861 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat 7921 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg 7981 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat 8041 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta 8101 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa 8161 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca 8221 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc 8281 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttgaa 8341 ttctctagaa ttaccctgtt atccctaggt ttaccgagct cttattggtt ttcaaacttc 8401 attgactgtg cccaacgcgc ctgggagctc acggggacag ccccccccca aagcccccag 8461 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc 8521 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg 8581 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga 8641 cacctggggg gatacgggga aaaagctagg ctgaaagacc ggttcttaga atgacacgcg 8701 cctgggagct cacggggaca gccccccccc aaagccccca gggatgtaat tacgtccctc 8761 ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg 8821 catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg 8881 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg 8941 aaaaagctaa cttctgagac gacaactttg tatagaaaag ttggctccga attcgccctt 9001 gacagcagag atccagtttg gttaattaat tggctccggt gcccgtcagt gggcagagcg 9061 cacatcgccc acagtccccg agaagttgtg gggaggggtc ggcaattgaa ccggtgccta 9121 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc 9181 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa 9241 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt 9301 tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct 9361 tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc 9421 cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat 9481 ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt 9541 ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga 9601 tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca 9661 gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta 9721 gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc 9781 ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc 9841 cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga 9901 gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac 9961 ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc 10021 tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac 10081 tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt 10141 tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca 10201 ggtgtcgtga gaagggcgaa ttcgacccaa gtttgtacaa aaaagcaggc tggatccagg 10261 tgccaccatg gtgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg 10321 caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg 10381 cacccagacc atgagaatca aggcggtcga gggcggccct ctccccttcg ccttcgacat 10441 cctggctacc agcttcatgt acggcagcaa aaccttcatc aaccacaccc agggcatccc 10501 cgacttcttt aagcagtcct tccccgaggg cttcacatgg gagagagtca ccacatacga 10561 agatgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta 10621 caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac 10681 actcggctgg gaggcctcca ccgagacact gtaccccgct gacggcggcc tggaaggcag 10741 agccgacatg gccctgaagc tcgtgggcgg gggccacctg atctgcaacc ttaagaccac 10801 atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacag 10861 gagactggaa agaatcaagg aggccgacaa agagacatac gtcgagcagc acgaggtggc 10921 tgtggccaga tactgcgacc tccctagcaa actggggcac aaacttaatt gagaattcta 10981 ccacccagct ttcttgtaca aagtggtacg tctcaagacc agaggtctct //