LOCUS BBa_K2100054 11038 bp DNA linear 25-Nov-2024 DEFINITION Sensor 18A ACCESSION BBa_K2100054 VERSION BBa_K2100054.1 FEATURES Location/Qualifiers misc_feature 1..92 /label=miR-18A target site promoter 1463..2636 /label=hEF1A CDS 2692..3411 /label=eBFP promoter 9032..10205 /label=hEF1A misc_feature 10270..10974 /label=mKate ORIGIN 1 ccagaaggag cacttagggc agtccagaag gagcacttag ggcagtccag aaggagcact 61 tagggcagtc cagaaggagc acttagggca gtttcaacca gaagagacct gtgcttcact 121 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac 181 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt 241 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat 301 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat 361 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt 421 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat 481 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct 541 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga 601 ctactcccag tcatagctgt ccctcttctc ttatggagat cccgagcggt gcgtttttat 661 gcttgtagta ttgtataatg tttttaagat ccaagcttct gacctcttct cttcctccca 721 cagggcctcg agacaattgc atcgatggta ccagtgaaag tctgcaatga attcgagttg 781 gcgcgcctgt cattctaaat ctctctttca gcctaaagct ttttccccgt atccccccag 841 gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct 901 tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga 961 ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca 1021 tccctggggg ctttgggagg gggctgtccc cgtgagctcc caggcgcgcc tgtcattcta 1081 aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg caggctcaaa 1141 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg 1201 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg 1261 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg 1321 ggggggctgt ccccgtgagc tcccaggcgc gccaactcgg taccgtatcg atgtcgacgt 1381 taacgctagt gatatcaact ttgtatagaa aagttggctc cgaattcgcc cttgacagca 1441 gagatccagt ttggttaatt aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg 1501 cccacagtcc ccgagaagtt gtggggaggg gtcggcaatt gaaccggtgc ctagagaagg 1561 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 1621 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 1681 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt 1741 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc 1801 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg 1861 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg 1921 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga 1981 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac 2041 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca 2101 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa 2161 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg 2221 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct 2281 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc 2341 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac 2401 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt 2461 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt 2521 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct 2581 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg 2641 tgagaagggc gaattcgacc caagtttgta caaaaaagca ggctcgccac catggtgagc 2701 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 2761 aacggccaca agttcagcgt gaggggcgag ggcgagggcg atgccaccaa cggcaagctg 2821 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 2881 accctgagcc acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac 2941 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 3001 gacggcacct acaagacccg cgccgaggtg aagttcgagg gcgacaccct agtgaaccgc 3061 atcgagctga agggcgtcga cttcaaggag gacggcaaca tcctggggca caagctggag 3121 tacaacttca acagccacaa catctatatc atggccgtca agcagaagaa cggcatcaag 3181 gtgaacttca agatccgcca caacgtggag gacggcagcg tgcagctcgc cgaccactac 3241 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacagcca ctacctgagc 3301 acccagtccg tgctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 3361 ttccgcaccg ccgccgggat cactctcggc atggacgagc tgtacaagta ataccaccca 3421 gctttcttgt acaaagtggt acgcgtgaat tcactcctca ggtgcaggct gcctatcaga 3481 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct 3541 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa 3601 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga 3661 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa 3721 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata 3781 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga 3841 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac 3901 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc 3961 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg 4021 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 4081 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 4141 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tcgacagtac taagcttggt 4201 ctaataccca atctctcgtc ttatccagat gttttatacg cctctggccc gtgtctcaaa 4261 atctctgatg ttacattgca caagataaaa taatatcatc atgaacaata aaactgtctg 4321 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc 4381 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg 4441 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt 4501 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa 4561 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg 4621 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat 4681 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt 4741 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc 4801 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct 4861 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag 4921 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag 4981 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat 5041 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta 5101 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat 5161 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg 5221 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagagg 5281 tatcactagt attacagagg taagttataa cagtcgccta acccaattga ggggataacg 5341 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 5401 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 5461 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 5521 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 5581 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 5641 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 5701 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 5761 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 5821 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 5881 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 5941 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 6001 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 6061 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 6121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 6181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 6241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 6301 tgataccgcg agaaccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 6361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 6421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 6481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 6541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 6601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 6661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 6721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 6781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 6841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 6901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 6961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 7021 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 7081 tgagcggata catatttgaa tgtagctccg aatttctcga gggcttgtcg acgacggcgg 7141 tctccgtcgt caggatcatg aattcgaccc gcctgccacc atgaccgagt acaagcccac 7201 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt 7261 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac 7321 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 7381 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 7441 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 7501 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 7561 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 7621 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacatccg cgccccgcaa 7681 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 7741 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga tacgcgtgaa ttcactcctc 7801 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat 7861 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc 7921 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt 7981 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt 8041 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc 8101 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa 8161 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa 8221 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac 8281 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg 8341 aattctctag aattaccctg ttatccctag gtttaccgag ctcttattgg ttttcaaact 8401 tcattgactg tgcccaacgc gcctgggagc tcacggggac agcccccccc caaagccccc 8461 agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc ggggctccgc 8521 tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca gcccgggcac 8581 ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt tgagcctgca 8641 gacacctggg gggatacggg gaaaaagcta ggctgaaaga ccggttctta gaatgacacg 8701 cgcctgggag ctcacgggga cagccccccc ccaaagcccc cagggatgta attacgtccc 8761 tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc 8821 cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat 8881 cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg 8941 ggaaaaagct aacttctgag acgacaactt tgtatagaaa agttggctcc gaattcgccc 9001 ttgacagcag agatccagtt tggttaatta attggctccg gtgcccgtca gtgggcagag 9061 cgcacatcgc ccacagtccc cgagaagttg tggggagggg tcggcaattg aaccggtgcc 9121 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 9181 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 9241 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc 9301 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt 9361 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 9421 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 9481 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 9541 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 9601 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 9661 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 9721 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 9781 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 9841 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 9901 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 9961 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 10021 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 10081 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 10141 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 10201 caggtgtcgt gagaagggcg aattcgaccc aagtttgtac aaaaaagcag gctggatcca 10261 ggtgccacca tggtgagcga gctgattaag gagaacatgc acatgaagct gtacatggag 10321 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag 10381 ggcacccaga ccatgagaat caaggcggtc gagggcggcc ctctcccctt cgccttcgac 10441 atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac ccagggcatc 10501 cccgacttct ttaagcagtc cttccccgag ggcttcacat gggagagagt caccacatac 10561 gaagatgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc 10621 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa 10681 acactcggct gggaggcctc caccgagaca ctgtaccccg ctgacggcgg cctggaaggc 10741 agagccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa ccttaagacc 10801 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac 10861 aggagactgg aaagaatcaa ggaggccgac aaagagacat acgtcgagca gcacgaggtg 10921 gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa ttgagaattc 10981 taccacccag ctttcttgta caaagtggta cgtctcaaga ccagaggtct ctgcttaa //