LOCUS BBa_K2100058 11038 bp DNA linear 23-Dec-2024 DEFINITION Sensor 34C ACCESSION BBa_K2100058 VERSION BBa_K2100058.1 FEATURES Location/Qualifiers misc_feature 1..88 /label=miR-34c-target site promoter 1457..2630 /label=hEF1A CDS 2686..3405 /label=eBFP promoter 9026..10199 /label=hEF1a CDS 10264..10968 /label=mKate ORIGIN 1 cctggccgtg tggttagtga ttcctggccg tgtggttagt gattcctggc cgtgtggtta 61 gtgattcctg gccgtgtggt tagtgattca accagaagag acctgtgctt cactcctcag 121 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac 181 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat 241 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg 301 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat 361 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta 421 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa 481 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca 541 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc 601 ccagtcatag ctgtccctct tctcttatgg agatcccgag cggtgcgttt ttatgcttgt 661 agtattgtat aatgttttta agatccaagc ttctgacctc ttctcttcct cccacagggc 721 ctcgagacaa ttgcatcgat ggtaccagtg aaagtctgca atgaattcga gttggcgcgc 781 ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct 841 gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg 901 tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag 961 cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg 1021 ggggctttgg gagggggctg tccccgtgag ctcccaggcg cgcctgtcat tctaaatctc 1081 tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag 1141 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg 1201 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct 1261 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg 1321 ctgtccccgt gagctcccag gcgcgccaac tcggtaccgt atcgatgtcg acgttaacgc 1381 tagtgatatc aactttgtat agaaaagttg gctccgaatt cgcccttgac agcagagatc 1441 cagtttggtt aattaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca 1501 gtccccgaga agttgtgggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc 1561 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga 1621 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc 1681 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc 1741 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt 1801 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt 1861 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt 1921 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct 1981 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt 2041 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg 2101 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc 2161 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg 2221 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca 2281 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa 2341 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg 2401 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg 2461 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca 2521 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc 2581 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagaa 2641 gggcgaattc gacccaagtt tgtacaaaaa agcaggctcg ccaccatggt gagcaagggc 2701 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 2761 cacaagttca gcgtgagggg cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg 2821 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 2881 agccacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc 2941 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 3001 acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctagtgaa ccgcatcgag 3061 ctgaagggcg tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 3121 ttcaacagcc acaacatcta tatcatggcc gtcaagcaga agaacggcat caaggtgaac 3181 ttcaagatcc gccacaacgt ggaggacggc agcgtgcagc tcgccgacca ctaccagcag 3241 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca gccactacct gagcacccag 3301 tccgtgctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttccgc 3361 accgccgccg ggatcactct cggcatggac gagctgtaca agtaatacca cccagctttc 3421 ttgtacaaag tggtacgcgt gaattcactc ctcaggtgca ggctgcctat cagaaggtgg 3481 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca 3541 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat 3601 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg 3661 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg 3721 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac 3781 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt 3841 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt 3901 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct 3961 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct 4021 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 4081 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 4141 gctttccagt cgggaaacct gtcgtgccag cggatcgaca gtactaagct tggtctaata 4201 cccaatctct cgtcttatcc agatgtttta tacgcctctg gcccgtgtct caaaatctct 4261 gatgttacat tgcacaagat aaaataatat catcatgaac aataaaactg tctgcttaca 4321 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat 4381 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc 4441 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga 4501 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc 4561 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat 4621 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg 4681 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc 4741 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac 4801 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg 4861 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca 4921 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta 4981 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact 5041 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata 5101 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat 5161 tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct 5221 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaggtatcac 5281 tagtattaca gaggtaagtt ataacagtcg cctaacccaa ttgaggggat aacgcaggaa 5341 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 5401 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 5461 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 5521 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 5581 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 5641 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 5701 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 5761 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 5821 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 5881 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 5941 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 6001 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 6061 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 6121 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 6181 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 6241 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 6301 cgcgagaacc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 6361 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 6421 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 6481 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 6541 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 6601 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 6661 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 6721 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 6781 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 6841 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 6901 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 6961 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 7021 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 7081 gatacatatt tgaatgtagc tccgaatttc tcgagggctt gtcgacgacg gcggtctccg 7141 tcgtcaggat catgaattcg acccgcctgc caccatgacc gagtacaagc ccacggtgcg 7201 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga 7261 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct 7321 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 7381 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 7441 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 7501 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 7561 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 7621 ggcggccgag cgcgccgggg tgcccgcctt cctggagaca tccgcgcccc gcaacctccc 7681 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 7741 cacctggtgc atgacccgca agcccggtgc ctgatacgcg tgaattcact cctcaggtgc 7801 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact 7861 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga 7921 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc 7981 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg 8041 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa 8101 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct 8161 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc 8221 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag 8281 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttgaattct 8341 ctagaattac cctgttatcc ctaggtttac cgagctctta ttggttttca aacttcattg 8401 actgtgccca acgcgcctgg gagctcacgg ggacagcccc cccccaaagc ccccagggat 8461 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt 8521 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa 8581 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc 8641 tggggggata cggggaaaaa gctaggctga aagaccggtt cttagaatga cacgcgcctg 8701 ggagctcacg gggacagccc ccccccaaag cccccaggga tgtaattacg tccctccccc 8761 gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc 8821 cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt 8881 cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat acggggaaaa 8941 agctaacttc tgagacgaca actttgtata gaaaagttgg ctccgaattc gcccttgaca 9001 gcagagatcc agtttggtta attaattggc tccggtgccc gtcagtgggc agagcgcaca 9061 tcgcccacag tccccgagaa gttgtgggga ggggtcggca attgaaccgg tgcctagaga 9121 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 9181 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 9241 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg 9301 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat 9361 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct 9421 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg 9481 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga 9541 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg 9601 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc 9661 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct 9721 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg 9781 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc 9841 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca 9901 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag 9961 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta 10021 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa 10081 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga 10141 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg 10201 tcgtgagaag ggcgaattcg acccaagttt gtacaaaaaa gcaggctgga tccaggtgcc 10261 accatggtga gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc 10321 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc 10381 cagaccatga gaatcaaggc ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg 10441 gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc acacccaggg catccccgac 10501 ttctttaagc agtccttccc cgagggcttc acatgggaga gagtcaccac atacgaagat 10561 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac 10621 gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc 10681 ggctgggagg cctccaccga gacactgtac cccgctgacg gcggcctgga aggcagagcc 10741 gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaaccttaa gaccacatac 10801 agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt ggacaggaga 10861 ctggaaagaa tcaaggaggc cgacaaagag acatacgtcg agcagcacga ggtggctgtg 10921 gccagatact gcgacctccc tagcaaactg gggcacaaac ttaattgaga attctaccac 10981 ccagctttct tgtacaaagt ggtacgtctc aagaccagag gtctctgctt aagtcttc //