LOCUS BBa_K2100060 11046 bp DNA linear 26-Nov-2024 DEFINITION Sensor 21-5p ACCESSION BBa_K2100060 VERSION BBa_K2100060.1 FEATURES Location/Qualifiers misc_feature 1..88 /label=miR-21-5p target site promoter 1465..2638 /label=hEF1A CDS 2694..3413 /label=eBFP promoter 9034..10207 /label=hEF1A CDS 10272..10976 /label=mKate ORIGIN 1 tagcttatca gactgatgtt gatagcttat cagactgatg ttgatagctt atcagactga 61 tgttgatagc ttatcagact gatgttgaga agacttcaac cagaagagac ctgtgcttca 121 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc 181 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc 241 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga 301 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga 361 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag 421 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc 481 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt 541 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct 601 gactactccc agtcatagct gtccctcttc tcttatggag atcccgagcg gtgcgttttt 661 atgcttgtag tattgtataa tgtttttaag atccaagctt ctgacctctt ctcttcctcc 721 cacagggcct cgagacaatt gcatcgatgg taccagtgaa agtctgcaat gaattcgagt 781 tggcgcgcct gtcattctaa atctctcttt cagcctaaag ctttttcccc gtatcccccc 841 aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac 901 cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg 961 gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta 1021 catccctggg ggctttggga gggggctgtc cccgtgagct cccaggcgcg cctgtcattc 1081 taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc tgcaggctca 1141 aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc 1201 tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc 1261 gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg 1321 ggggggggct gtccccgtga gctcccaggc gcgccaactc ggtaccgtat cgatgtcgac 1381 gttaacgcta gtgatatcaa ctttgtatag aaaagttggc tccgaattcg cccttgacag 1441 cagagatcca gtttggttaa ttaattggct ccggtgcccg tcagtgggca gagcgcacat 1501 cgcccacagt ccccgagaag ttgtggggag gggtcggcaa ttgaaccggt gcctagagaa 1561 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg 1621 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt 1681 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg 1741 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc 1801 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt 1861 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt 1921 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat 1981 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc 2041 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca 2101 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc 2161 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg 2221 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc 2281 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac 2341 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt 2401 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag 2461 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag 2521 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat 2581 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt 2641 cgtgagaagg gcgaattcga cccaagtttg tacaaaaaag caggctcgcc accatggtga 2701 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg 2761 taaacggcca caagttcagc gtgaggggcg agggcgaggg cgatgccacc aacggcaagc 2821 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga 2881 ccaccctgag ccacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg 2941 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg 3001 acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctagtgaacc 3061 gcatcgagct gaagggcgtc gacttcaagg aggacggcaa catcctgggg cacaagctgg 3121 agtacaactt caacagccac aacatctata tcatggccgt caagcagaag aacggcatca 3181 aggtgaactt caagatccgc cacaacgtgg aggacggcag cgtgcagctc gccgaccact 3241 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacagc cactacctga 3301 gcacccagtc cgtgctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg 3361 agttccgcac cgccgccggg atcactctcg gcatggacga gctgtacaag taataccacc 3421 cagctttctt gtacaaagtg gtacgcgtga attcactcct caggtgcagg ctgcctatca 3481 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc 3541 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata 3601 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag 3661 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc 3721 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta 3781 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta 3841 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt 3901 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc 3961 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc 4021 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 4081 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 4141 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatcgacagt actaagcttg 4201 gtctaatacc caatctctcg tcttatccag atgttttata cgcctctggc ccgtgtctca 4261 aaatctctga tgttacattg cacaagataa aataatatca tcatgaacaa taaaactgtc 4321 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag 4381 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 4441 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt 4501 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 4561 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 4621 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga 4681 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca 4741 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc 4801 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg 4861 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc 4921 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat 4981 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct 5041 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg 5101 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta 5161 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg 5221 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga 5281 ggtatcacta gtattacaga ggtaagttat aacagtcgcc taacccaatt gaggggataa 5341 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 5401 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 5461 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 5521 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 5581 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 5641 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 5701 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 5761 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 5821 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 5881 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 5941 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 6001 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 6061 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 6121 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 6181 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 6241 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 6301 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc 6361 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 6421 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 6481 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 6541 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 6601 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 6661 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 6721 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 6781 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 6841 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 6901 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 6961 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 7021 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 7081 catgagcgga tacatatttg aatgtagctc cgaatttctc gagggcttgt cgacgacggc 7141 ggtctccgtc gtcaggatca tgaattcgac ccgcctgcca ccatgaccga gtacaagccc 7201 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg 7261 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc 7321 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 7381 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 7441 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 7501 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 7561 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 7621 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacatc cgcgccccgc 7681 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 7741 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gatacgcgtg aattcactcc 7801 tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa 7861 ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga 7921 gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt 7981 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga 8041 gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg 8101 gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag 8161 aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt 8221 aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact 8281 actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct 8341 tgaattctct agaattaccc tgttatccct aggtttaccg agctcttatt ggttttcaaa 8401 cttcattgac tgtgcccaac gcgcctggga gctcacgggg acagcccccc cccaaagccc 8461 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc 8521 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc 8581 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg 8641 cagacacctg gggggatacg gggaaaaagc taggctgaaa gaccggttct tagaatgaca 8701 cgcgcctggg agctcacggg gacagccccc ccccaaagcc cccagggatg taattacgtc 8761 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc 8821 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg 8881 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac 8941 ggggaaaaag ctaacttctg agacgacaac tttgtataga aaagttggct ccgaattcgc 9001 ccttgacagc agagatccag tttggttaat taattggctc cggtgcccgt cagtgggcag 9061 agcgcacatc gcccacagtc cccgagaagt tgtggggagg ggtcggcaat tgaaccggtg 9121 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt 9181 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc 9241 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc 9301 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga 9361 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag 9421 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc 9481 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa 9541 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc 9601 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt 9661 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg 9721 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc 9781 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc 9841 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg 9901 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact 9961 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt 10021 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg 10081 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg 10141 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat 10201 ttcaggtgtc gtgagaaggg cgaattcgac ccaagtttgt acaaaaaagc aggctggatc 10261 caggtgccac catggtgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg 10321 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg 10381 agggcaccca gaccatgaga atcaaggcgg tcgagggcgg ccctctcccc ttcgccttcg 10441 acatcctggc taccagcttc atgtacggca gcaaaacctt catcaaccac acccagggca 10501 tccccgactt ctttaagcag tccttccccg agggcttcac atgggagaga gtcaccacat 10561 acgaagatgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca 10621 tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga 10681 aaacactcgg ctgggaggcc tccaccgaga cactgtaccc cgctgacggc ggcctggaag 10741 gcagagccga catggccctg aagctcgtgg gcgggggcca cctgatctgc aaccttaaga 10801 ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactatgtgg 10861 acaggagact ggaaagaatc aaggaggccg acaaagagac atacgtcgag cagcacgagg 10921 tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaaactt aattgagaat 10981 tctaccaccc agctttcttg tacaaagtgg tacgtctcaa gaccagaggt ctctgcttaa 11041 gtcttc //