BBa_K2100062
1
BBa_K2100062
pEXPR EGSH: TP901
2016-10-18T11:00:00Z
2016-10-20T12:10:03Z
[[Part:BBa_K2100021|pENTR pEGSH]]
[[Part:BBa_K2100019|pENTR TP901]]
This is a final expression vector that we used to transfect into mammalian cells for our experiments. TP901 is a serine recombinase, which we used as biological latches to control expression of a final out put gene for our genetic circuit. pEGSH is an inducible promoter similar to pTRE. This inducible promoter system require the presence of VgEcr and RXR protein and small molecule Ponasterone A (PonA). By putting TP901 recombinase under pEGSH, we can control the expression of TP901 with small molecule PonA.
true
false
_2568_
32435
32889
9
false
We used LR reaction to put to clone the promoter and gene to a backbone vector
false
Trinh Nguyen
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z