BBa_K2100068
1
BBa_K2100068
pENTR L7Ae
2016-10-18T11:00:00Z
2016-10-23T12:22:35Z
Weiss Lab Library
This entry vector contains L7Ae, which is used in conjunction with k-turns to suppress expression of genes.
false
false
_2568_
32891
31612
9
false
None
false
Colleen Foley
BBa_K2100069
1
BBa_K2100069
pEXPR TRE: L7Ae
2016-10-18T11:00:00Z
2016-10-23T12:40:36Z
We synthesized this part.
This part is L7Ae under Dox-inducible promoter TRE
false
false
_2568_
32891
31612
9
false
None
false
Colleen Foley
component2528651
1
BBa_K2100068
component2528650
1
BBa_K2100004
annotation2528651
1
BBa_K2100068
range2528651
1
337
831
annotation2528650
1
BBa_K2100004
range2528650
1
1
330
BBa_K2100004
1
BBa_K2100004
pENTR pTRE
2016-10-09T11:00:00Z
2016-10-10T09:55:39Z
This is a synthetic promoter.
description here!
false
false
_2568_
31606
31606
9
false
This part was created using scarless golden gate assembly. This basic part is flanked by L1 and L2 sites and can be easily cloned into an entry vector using an LR reaction. A promoter can be easily inserted in front of this part in a one pot LR reaction with a promoter flanked by L4 and R1 sites cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
false
Kathleen Brandes
annotation2490227
1
tetO binding site
range2490227
1
77
91
annotation2490247
1
tetO binding site
range2490247
1
187
201
annotation2490245
1
tetO binding site
range2490245
1
44
58
annotation2490217
1
minCMV
range2490217
1
253
312
annotation2490244
1
tetO binding site
range2490244
1
222
236
annotation2490246
1
tetO binding site
range2490246
1
116
130
annotation2490243
1
tetO binding site
range2490243
1
150
164
BBa_K2100068_sequence
1
atgtacgtgagatttgaggttcctgaggacatgcagaacgaagctctgagtctgctggagaaggttagggagagcggtaaggtaaagaaaggtaccaacgagacgacaaaggctgtggagaggggactggcaaagctcgtttacatcgcagaggatgttgacccgcctgagatcgttgctcatctgcccctcctctgcgaggagaagaatgtgccgtacatttacgttaaaagcaagaacgaccttggaagggctgtgggcattgaggtgccatgcgcttcggcagcgataatcaacgagggagagctgagaaaggagcttggaagccttgtggagaagattaaaggccttcagaagtaaaaagcaagaacgaccttggaagggctgtgggcattgaggtgccatgcgcttcggcagcgataatcaacgagggagagctgagaaaggagcttggaagccttgtggagaagattaaaggccttcagaagtaatacc
BBa_K2100069_sequence
1
gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgactactagatgtacgtgagatttgaggttcctgaggacatgcagaacgaagctctgagtctgctggagaaggttagggagagcggtaaggtaaagaaaggtaccaacgagacgacaaaggctgtggagaggggactggcaaagctcgtttacatcgcagaggatgttgacccgcctgagatcgttgctcatctgcccctcctctgcgaggagaagaatgtgccgtacatttacgttaaaagcaagaacgaccttggaagggctgtgggcattgaggtgccatgcgcttcggcagcgataatcaacgagggagagctgagaaaggagcttggaagccttgtggagaagattaaaggccttcagaagtaaaaagcaagaacgaccttggaagggctgtgggcattgaggtgccatgcgcttcggcagcgataatcaacgagggagagctgagaaaggagcttggaagccttgtggagaagattaaaggccttcagaagtaatacc
BBa_K2100004_sequence
1
gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z