LOCUS BBa_K2100070 4668 bp DNA linear 22-Nov-2024 DEFINITION pEXPR hEF1a: attB-flipped eYFP-attP ACCESSION BBa_K2100070 VERSION BBa_K2100070.1 FEATURES Location/Qualifiers misc_feature 1..1218 /label=BBa_K2100003 CDS 2588..3307 /label=flipped eYFP misc_feature 2534..2583 /label=TP901 attB misc_feature 2402..2500 /label=L1 misc_feature 3325..3374 /label=TP901 attP misc_feature 3402..3497 /label=L2 misc_feature 3668..4477 /label=Kan(R) misc_feature 1227..4668 /label=BBa_K2100049 ORIGIN 1 gctccgaatt cgcccttatt aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg 61 cccacagtcc ccgagaagtt gtggggaggg gtcggcaatt gaaccggtgc ctagagaagg 121 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 181 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 241 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt 301 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc 361 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg 421 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg 481 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga 541 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac 601 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca 661 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa 721 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg 781 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct 841 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc 901 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac 961 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt 1021 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt 1081 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct 1141 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtggat 1201 cccaagggcg aattcgacta ctagagaaaa ccaccgctac cagcggtggt ttgtttgccg 1261 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 1321 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 1381 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 1441 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 1501 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 1561 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 1621 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 1681 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 1741 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 1801 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 1861 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 1921 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 1981 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 2041 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa 2101 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc 2161 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat 2221 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt 2281 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta 2341 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc 2401 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa 2461 tgctttttta taatgccaac tttgtacaaa aaagcaggct ggatcccggt accctcgaca 2521 cctgctgaca ggtataattg ccaacacaat taacatctca atcaaggtaa atgctttttg 2581 cttagtctta cttatacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact 2641 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactggtagc 2701 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt 2761 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct 2821 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact 2881 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc 2941 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt 3001 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt 3061 cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag ccgaaggtgg 3121 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca 3181 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta 3241 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc 3301 tcaccatggt ggcgacaggt gagcttgcga gtttttattt cgtttatttc aattaaggta 3361 actaaaaaac tcctgcttca gtgcaggtga attctaccac ccagctttct tgtacaaagt 3421 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca 3481 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt 3541 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca 3601 agataaaata atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag 3661 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga 3721 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat 3781 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag 3841 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc 3901 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc 3961 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat 4021 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc 4081 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt 4141 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa 4201 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc 4261 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt 4321 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc 4381 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa 4441 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta 4501 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct 4561 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 4621 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaa //