BBa_K2100050 1 BBa_K2100050 pENTR L4_EGSH 2x k-turn_R1 2016-10-18T11:00:00Z 2016-10-23T12:49:37Z EGSH was obtained using PCR extension from pENTR pEGSH (BBa_K2100021). The k-turn sequence was obtained from addgene (plasmids deposite by Dr. Ron Weiss' lab). We ordered the k-turns as single-stranded oligos with different Q sites for Golden Gate reaction. This basic part entry vector is flanked by L4 and R1 sites, which are used to denote a promoter. This can be cascade with a gene (flanked by L1, L2 sites) using an LR reaction and cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts. k-turn stands for kinh turn motif in the messenger RNA. The k-turn motif is a part of the L7Ae/k-turn RNA-based repressing system. By putting the k-turn motifs in pENTR promoter vector, the k-turns will be added into the 5'UTR of the gene after LR reaction. The k-turn adds in a higher gene regulation level to reduce the basal expression of the gene downstream due to leaky expression of the EGSH promoter false false _2568_ 32889 32889 9 false The distance from the k-turn repeats to the open reading frame of the downstream gene determines the efficiency of L7Ae/k-turn repressing system. Thus, we were considering adding k-turns right after the promoter (in the pENTR promoter vector) or right in front of the gene coding sequence (in the pENTR gene vector). false Trinh Nguyen annotation2522960 1 k-turn range2522960 1 1974 1996 annotation2522939 1 k-turn range2522939 1 1947 1969 annotation2528653 1 R1 range2528653 1 2017 2145 annotation2528652 1 L4 range2528652 1 1185 1281 annotation2522924 1 pEGSH range2522924 1 1410 1931 BBa_K2100019 1 BBa_K2100019 pENTR TP901 2016-10-13T11:00:00Z 2016-10-14T04:53:04Z This is from the mammalian genome. description here. false false _2568_ 31606 31606 9 false This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. This can be cascade with a promoter (flanked by L4, R1 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts. false Kathleen Brandes BBa_K2100074 1 BBa_K2100074 pEXPR EGSH - 2kturn: TP901 2016-10-18T11:00:00Z 2016-10-19T07:35:28Z We synthesized this part. This part contains 2 k-turns to repress serine integrase TP901. false false _2568_ 31612 31612 9 false None false Colleen Foley component2523865 1 BBa_K2100019 component2523864 1 BBa_K2100050 annotation2523864 1 BBa_K2100050 range2523864 1 1 3387 annotation2523865 1 BBa_K2100019 range2523865 1 3396 4924 BBa_K2100074_sequence 1 ccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatacgcgtaccgctagccaggaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttagtttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcacaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtcttccgactgagcctttcgttttatttgatgcctggcagttccctactctcgcgttaacgctagcatggatgttttcccagtcacgacgttgtaaaacgacggccagtcttaagctcgggcccgagttaacgctaccatggagctccaaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgataagcaatgcttttttataatgccaactttgtatagaaaagttggctccgaattcacctgctgacaggtagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttacgcgtatagatctcgcatattaagtgcattgttctcgataccgctaagtgcattgttctcgttagctcgatggacaagtgcattgttctcttgctgaaagctcgatggacaagtgcattgttctcttgctgaaagctcgatggacaagtgcattgttctcttgctgaaagctcagtacccgggtcggagtactgccccgcccctagcgattagccccggccccgcatagctccgccccgggagtaccctcgaccgccggagtataaatagaggcgcttcgtctacggagcgacaattcaattcaaacaagcaaagtgaacacgtcgctaagcgaaagctaagcaaataaacaagcgcagctgaacaagctaaacaatctgcagtaaagtgcaagttaaagtgaatcaattaaaagtaaccagcaaccaagtaaatcaactgcaactactgaaatctgccaagaagtaattattgaatacaagaagacaactctgaatactttcaaaagttaccgagaaagaagaactcagacacagcagacagggggcgtgatccgaaaggtgacccagtcgggcgtgatccgaaaggtgacccgcttcagtgcaggtgagcttcgacccaagtttgtacaaaaaagttgaacgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaacacaacatatgcagtcactatgaaccaactacttagatggtattagtgacctgtagaattaattcgagctctagagctgcagggcggccgcgatatcccctatagtgagtcgtattacatggtcatagctgtttcctggcagctctggcccgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgacgggacggcgcaagctcatgaccaaaatcccttaacgtgagttacgcgtcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctatactagagggatcccggtaccaggtgccaccatgactaagaaggtagcaatttacacccgcgtatctacaaccaaccaggcagaggaaggtttctccattgatgaacaaatagatcgactcactaagtacgcagaagccatggggtggcaagtgagtgacacgtatacggacgccggcttttctggagccaagctggaaagaccagcgatgcaacgcctcataaacgatattgaaaacaaagcgtttgatacggtactcgtatataagttggaccgcttgagccgatccgtacgcgacacgctttatttggtgaaggacgtctttactaaaaataagatagactttatttcactcaatgaatcaattgacacaagtagtgcgatgggttcactttttctcacaatactgtccgctataaacgaattcgaacgggagaacataaaggagaggatgacaatggggaagctcggaagggccaagtctggcaaaagtatgatgtggacaaagactgctttcgggtactatcacaatcgaaagacgggtatactcgaaatcgtccctcttcaggcaacgatagtggaacagatatttacggattacctctcaggtattagtctcacgaagttgcgagataagctgaacgagtcaggtcacataggaaaggacatcccgtggtcttaccgaaccctgcgccaaacactggacaacccagtatattgtgggtacatcaagtttaaggattcattgttcgaaggaatgcacaaacctattatcccttacgaaacctatttgaaggtgcagaaggaattggaggaacggcaacaacagacgtacgagcggaataacaacccgaggcccttccaggctaagtatatgctctctgggatggctcggtgtggatactgtggtgcgcctctgaagattgtcttgggacacaagcgaaaggatggtagccggacaatgaaatatcattgtgcaaacagattccctcgcaaaacaaaaggaatcacagtgtataacgacaataaaaaatgtgactccgggacgtatgatctcagtaacttggagaacaccgtgatagataatctcataggctttcaggagaacaacgattcattgctcaagatcatcaacggtaacaaccaacccattcttgacacttcaagcttcaagaagcagatatctcagattgacaaaaagatccaaaagaatagcgatttgtatctcaatgacttcatcactatggatgaattgaaagataggacagactccctccaggcagaaaaaaagctccttaaggcaaagatctcagagaacaaatttaatgactctacggacgtgttcgagcttgtgaaaacccaacttggttctatacccataaacgagttgtcctacgacaataagaaaaagatagtcaacaatctggtgagtaaagtcgacgtaaccgctgacaatgttgatataatattcaaattccagctcgcaccaaagaagaaacgcaaggtttaggcttcagtgcaggtgaattctaccacc BBa_K2100019_sequence 1 ggatcccggtaccaggtgccaccatgactaagaaggtagcaatttacacccgcgtatctacaaccaaccaggcagaggaaggtttctccattgatgaacaaatagatcgactcactaagtacgcagaagccatggggtggcaagtgagtgacacgtatacggacgccggcttttctggagccaagctggaaagaccagcgatgcaacgcctcataaacgatattgaaaacaaagcgtttgatacggtactcgtatataagttggaccgcttgagccgatccgtacgcgacacgctttatttggtgaaggacgtctttactaaaaataagatagactttatttcactcaatgaatcaattgacacaagtagtgcgatgggttcactttttctcacaatactgtccgctataaacgaattcgaacgggagaacataaaggagaggatgacaatggggaagctcggaagggccaagtctggcaaaagtatgatgtggacaaagactgctttcgggtactatcacaatcgaaagacgggtatactcgaaatcgtccctcttcaggcaacgatagtggaacagatatttacggattacctctcaggtattagtctcacgaagttgcgagataagctgaacgagtcaggtcacataggaaaggacatcccgtggtcttaccgaaccctgcgccaaacactggacaacccagtatattgtgggtacatcaagtttaaggattcattgttcgaaggaatgcacaaacctattatcccttacgaaacctatttgaaggtgcagaaggaattggaggaacggcaacaacagacgtacgagcggaataacaacccgaggcccttccaggctaagtatatgctctctgggatggctcggtgtggatactgtggtgcgcctctgaagattgtcttgggacacaagcgaaaggatggtagccggacaatgaaatatcattgtgcaaacagattccctcgcaaaacaaaaggaatcacagtgtataacgacaataaaaaatgtgactccgggacgtatgatctcagtaacttggagaacaccgtgatagataatctcataggctttcaggagaacaacgattcattgctcaagatcatcaacggtaacaaccaacccattcttgacacttcaagcttcaagaagcagatatctcagattgacaaaaagatccaaaagaatagcgatttgtatctcaatgacttcatcactatggatgaattgaaagataggacagactccctccaggcagaaaaaaagctccttaaggcaaagatctcagagaacaaatttaatgactctacggacgtgttcgagcttgtgaaaacccaacttggttctatacccataaacgagttgtcctacgacaataagaaaaagatagtcaacaatctggtgagtaaagtcgacgtaaccgctgacaatgttgatataatattcaaattccagctcgcaccaaagaagaaacgcaaggtttaggcttcagtgcaggtgaattctaccacc BBa_K2100050_sequence 1 ccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatacgcgtaccgctagccaggaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttagtttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcacaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtcttccgactgagcctttcgttttatttgatgcctggcagttccctactctcgcgttaacgctagcatggatgttttcccagtcacgacgttgtaaaacgacggccagtcttaagctcgggcccgagttaacgctaccatggagctccaaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgataagcaatgcttttttataatgccaactttgtatagaaaagttggctccgaattcacctgctgacaggtagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttacgcgtatagatctcgcatattaagtgcattgttctcgataccgctaagtgcattgttctcgttagctcgatggacaagtgcattgttctcttgctgaaagctcgatggacaagtgcattgttctcttgctgaaagctcgatggacaagtgcattgttctcttgctgaaagctcagtacccgggtcggagtactgccccgcccctagcgattagccccggccccgcatagctccgccccgggagtaccctcgaccgccggagtataaatagaggcgcttcgtctacggagcgacaattcaattcaaacaagcaaagtgaacacgtcgctaagcgaaagctaagcaaataaacaagcgcagctgaacaagctaaacaatctgcagtaaagtgcaagttaaagtgaatcaattaaaagtaaccagcaaccaagtaaatcaactgcaactactgaaatctgccaagaagtaattattgaatacaagaagacaactctgaatactttcaaaagttaccgagaaagaagaactcagacacagcagacagggggcgtgatccgaaaggtgacccagtcgggcgtgatccgaaaggtgacccgcttcagtgcaggtgagcttcgacccaagtttgtacaaaaaagttgaacgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaacacaacatatgcagtcactatgaaccaactacttagatggtattagtgacctgtagaattaattcgagctctagagctgcagggcggccgcgatatcccctatagtgagtcgtattacatggtcatagctgtttcctggcagctctggcccgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgacgggacggcgcaagctcatgaccaaaatcccttaacgtgagttacgcgtcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgcta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z