BBa_K2100055
1
BBa_K2100055
pENTR L4_EGSH 4x k-turn_R1
2016-10-18T11:00:00Z
2016-10-19T06:04:27Z
EGSH was obtained using PCR extension from pENTR pEGSH (BBa_K2100021). The k-turn sequence was obtained from addgene (plasmids deposite by Dr. Ron Weiss' lab). We ordered the k-turns as single-stranded oligos with different Q sites for Golden Gate reaction.
This basic part entry vector is flanked by L4 and R1 sites, which are used to denote a promoter. This can be cascade with a gene (flanked by L1, L2 sites) using an LR reaction and cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
k-turn stands for kink turn motif in the messenger RNA. The k-turn motif is a part of the L7Ae/k-turn RNA-based repressing system. By putting the k-turn motifs in pENTR promoter vector, the k-turns will be added into the 5'UTR of the gene after LR reaction. The k-turn adds in a higher gene regulation level to reduce the basal expression of the gene downstream due to leaky expression of the EGSH promoter.
Theoretically, the number of k-turn motifs would affect the efficiency of L7Ae/k-turn repressing system. Thus, promoter with 4x k-turn would have higher efficiency in suppressing leaky expression than promoter with just 2x k-turn [[Part:BBa_K2100050|pENTR EGSH 2x k-turn]]
false
false
_2568_
32889
32889
9
It's complicated
false
The distance from the k-turn repeats to the open reading frame of the downstream gene determines the efficiency of L7Ae/k-turn repressing system. Thus, we were considering adding k-turns right after the promoter (in the pENTR promoter vector) or right in front of the gene coding sequence (in the pENTR gene vector).
false
Trinh Nguyen
annotation2530125
1
k-turn
range2530125
1
1952
1974
annotation2523219
1
pEGSH
range2523219
1
1388
1909
annotation2530127
1
k-turn
range2530127
1
2006
2028
annotation2530126
1
k-turn
range2530126
1
1979
2001
annotation2530124
1
k-turn
range2530124
1
1925
1947
BBa_K2100019
1
BBa_K2100019
pENTR TP901
2016-10-13T11:00:00Z
2016-10-14T04:53:04Z
This is from the mammalian genome.
description here.
false
false
_2568_
31606
31606
9
false
This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene. This can be cascade with a promoter (flanked by L4, R1 sites) using an LR reaction and cloning into a cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
false
Kathleen Brandes
BBa_K2100077
1
BBa_K2100077
pEXPR EGSH - 4kturn: TP901
2016-10-18T11:00:00Z
2016-10-19T07:55:02Z
We synthesized this part.
This part contains 4 k-turns to repress expression of serine integrase TP901.
false
false
_2568_
31612
31612
9
false
None
false
Colleen Foley
component2523895
1
BBa_K2100055
component2523896
1
BBa_K2100019
annotation2523896
1
BBa_K2100019
range2523896
1
3450
4978
annotation2523895
1
BBa_K2100055
range2523895
1
1
3441
BBa_K2100055_sequence
1
atcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatacgcgtaccgctagccaggaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttagtttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcacaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtcttccgactgagcctttcgttttatttgatgcctggcagttccctactctcgcgttaacgctagcatggatgttttcccagtcacgacgttgtaaaacgacggccagtcttaagctcgggcccgagttaacgctaccatggagctccaaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgataagcaatgcttttttataatgccaactttgtatagaaaagttggctccgaattcacctgctgacaggtagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttacgcgtatagatctcgcatattaagtgcattgttctcgataccgctaagtgcattgttctcgttagctcgatggacaagtgcattgttctcttgctgaaagctcgatggacaagtgcattgttctcttgctgaaagctcgatggacaagtgcattgttctcttgctgaaagctcagtacccgggtcggagtactgccccgcccctagcgattagccccggccccgcatagctccgccccgggagtaccctcgaccgccggagtataaatagaggcgcttcgtctacggagcgacaattcaattcaaacaagcaaagtgaacacgtcgctaagcgaaagctaagcaaataaacaagcgcagctgaacaagctaaacaatctgcagtaaagtgcaagttaaagtgaatcaattaaaagtaaccagcaaccaagtaaatcaactgcaactactgaaatctgccaagaagtaattattgaatacaagaagacaactctgaatactttcaaaagttaccgagaaagaagaactcagacacagcagacagggggcgtgatccgaaaggtgacccagtcgggcgtgatccgaaaggtgacccgagcgggcgtgatccgaaaggtgacccctgagggcgtgatccgaaaggtgacccgcttcagtgcaggtgagcttcgacccaagtttgtacaaaaaagttgaacgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaacacaacatatgcagtcactatgaaccaactacttagatggtattagtgacctgtagaattaattcgagctctagagctgcagggcggccgcgatatcccctatagtgagtcgtattacatggtcatagctgtttcctggcagctctggcccgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgacgggacggcgcaagctcatgaccaaaatcccttaacgtgagttacgcgtcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccgg
BBa_K2100077_sequence
1
atcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatacgcgtaccgctagccaggaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttagtttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcacaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtcttccgactgagcctttcgttttatttgatgcctggcagttccctactctcgcgttaacgctagcatggatgttttcccagtcacgacgttgtaaaacgacggccagtcttaagctcgggcccgagttaacgctaccatggagctccaaataatgattttattttgactgatagtgacctgttcgttgcaacaaattgataagcaatgcttttttataatgccaactttgtatagaaaagttggctccgaattcacctgctgacaggtagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttacgcgtatagatctcgcatattaagtgcattgttctcgataccgctaagtgcattgttctcgttagctcgatggacaagtgcattgttctcttgctgaaagctcgatggacaagtgcattgttctcttgctgaaagctcgatggacaagtgcattgttctcttgctgaaagctcagtacccgggtcggagtactgccccgcccctagcgattagccccggccccgcatagctccgccccgggagtaccctcgaccgccggagtataaatagaggcgcttcgtctacggagcgacaattcaattcaaacaagcaaagtgaacacgtcgctaagcgaaagctaagcaaataaacaagcgcagctgaacaagctaaacaatctgcagtaaagtgcaagttaaagtgaatcaattaaaagtaaccagcaaccaagtaaatcaactgcaactactgaaatctgccaagaagtaattattgaatacaagaagacaactctgaatactttcaaaagttaccgagaaagaagaactcagacacagcagacagggggcgtgatccgaaaggtgacccagtcgggcgtgatccgaaaggtgacccgagcgggcgtgatccgaaaggtgacccctgagggcgtgatccgaaaggtgacccgcttcagtgcaggtgagcttcgacccaagtttgtacaaaaaagttgaacgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaacacaacatatgcagtcactatgaaccaactacttagatggtattagtgacctgtagaattaattcgagctctagagctgcagggcggccgcgatatcccctatagtgagtcgtattacatggtcatagctgtttcctggcagctctggcccgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgacgggacggcgcaagctcatgaccaaaatcccttaacgtgagttacgcgtcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggtactagagggatcccggtaccaggtgccaccatgactaagaaggtagcaatttacacccgcgtatctacaaccaaccaggcagaggaaggtttctccattgatgaacaaatagatcgactcactaagtacgcagaagccatggggtggcaagtgagtgacacgtatacggacgccggcttttctggagccaagctggaaagaccagcgatgcaacgcctcataaacgatattgaaaacaaagcgtttgatacggtactcgtatataagttggaccgcttgagccgatccgtacgcgacacgctttatttggtgaaggacgtctttactaaaaataagatagactttatttcactcaatgaatcaattgacacaagtagtgcgatgggttcactttttctcacaatactgtccgctataaacgaattcgaacgggagaacataaaggagaggatgacaatggggaagctcggaagggccaagtctggcaaaagtatgatgtggacaaagactgctttcgggtactatcacaatcgaaagacgggtatactcgaaatcgtccctcttcaggcaacgatagtggaacagatatttacggattacctctcaggtattagtctcacgaagttgcgagataagctgaacgagtcaggtcacataggaaaggacatcccgtggtcttaccgaaccctgcgccaaacactggacaacccagtatattgtgggtacatcaagtttaaggattcattgttcgaaggaatgcacaaacctattatcccttacgaaacctatttgaaggtgcagaaggaattggaggaacggcaacaacagacgtacgagcggaataacaacccgaggcccttccaggctaagtatatgctctctgggatggctcggtgtggatactgtggtgcgcctctgaagattgtcttgggacacaagcgaaaggatggtagccggacaatgaaatatcattgtgcaaacagattccctcgcaaaacaaaaggaatcacagtgtataacgacaataaaaaatgtgactccgggacgtatgatctcagtaacttggagaacaccgtgatagataatctcataggctttcaggagaacaacgattcattgctcaagatcatcaacggtaacaaccaacccattcttgacacttcaagcttcaagaagcagatatctcagattgacaaaaagatccaaaagaatagcgatttgtatctcaatgacttcatcactatggatgaattgaaagataggacagactccctccaggcagaaaaaaagctccttaaggcaaagatctcagagaacaaatttaatgactctacggacgtgttcgagcttgtgaaaacccaacttggttctatacccataaacgagttgtcctacgacaataagaaaaagatagtcaacaatctggtgagtaaagtcgacgtaaccgctgacaatgttgatataatattcaaattccagctcgcaccaaagaagaaacgcaaggtttaggcttcagtgcaggtgaattctaccacc
BBa_K2100019_sequence
1
ggatcccggtaccaggtgccaccatgactaagaaggtagcaatttacacccgcgtatctacaaccaaccaggcagaggaaggtttctccattgatgaacaaatagatcgactcactaagtacgcagaagccatggggtggcaagtgagtgacacgtatacggacgccggcttttctggagccaagctggaaagaccagcgatgcaacgcctcataaacgatattgaaaacaaagcgtttgatacggtactcgtatataagttggaccgcttgagccgatccgtacgcgacacgctttatttggtgaaggacgtctttactaaaaataagatagactttatttcactcaatgaatcaattgacacaagtagtgcgatgggttcactttttctcacaatactgtccgctataaacgaattcgaacgggagaacataaaggagaggatgacaatggggaagctcggaagggccaagtctggcaaaagtatgatgtggacaaagactgctttcgggtactatcacaatcgaaagacgggtatactcgaaatcgtccctcttcaggcaacgatagtggaacagatatttacggattacctctcaggtattagtctcacgaagttgcgagataagctgaacgagtcaggtcacataggaaaggacatcccgtggtcttaccgaaccctgcgccaaacactggacaacccagtatattgtgggtacatcaagtttaaggattcattgttcgaaggaatgcacaaacctattatcccttacgaaacctatttgaaggtgcagaaggaattggaggaacggcaacaacagacgtacgagcggaataacaacccgaggcccttccaggctaagtatatgctctctgggatggctcggtgtggatactgtggtgcgcctctgaagattgtcttgggacacaagcgaaaggatggtagccggacaatgaaatatcattgtgcaaacagattccctcgcaaaacaaaaggaatcacagtgtataacgacaataaaaaatgtgactccgggacgtatgatctcagtaacttggagaacaccgtgatagataatctcataggctttcaggagaacaacgattcattgctcaagatcatcaacggtaacaaccaacccattcttgacacttcaagcttcaagaagcagatatctcagattgacaaaaagatccaaaagaatagcgatttgtatctcaatgacttcatcactatggatgaattgaaagataggacagactccctccaggcagaaaaaaagctccttaaggcaaagatctcagagaacaaatttaatgactctacggacgtgttcgagcttgtgaaaacccaacttggttctatacccataaacgagttgtcctacgacaataagaaaaagatagtcaacaatctggtgagtaaagtcgacgtaaccgctgacaatgttgatataatattcaaattccagctcgcaccaaagaagaaacgcaaggtttaggcttcagtgcaggtgaattctaccacc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z