BBa_K210009
1
BBa_K210009
HIV-TAT::(LALAAAA)3 generator
2009-10-14T11:00:00Z
2015-05-08T01:11:29Z
BBa_I712074,BBa_B0015
T7promoter(BBa_I712074)+HIV-TAT+his tag+(LALAAAA)??3+terminator(BBa_B0015)
(LALAAAA)??3 is a transmenbrene domein. The liposome which has HIV-TAT can intrude in mammalian cells.
We translated this parts by RTS with liposome, and ultracentrifuge in order to separate the liposome with HIV-TAT.
false
false
_346_
0
5425
9
It's complicated
true
This part is identified by his tag.
false
Makoto Kashima
annotation2042420
1
his tag
range2042420
1
109
144
annotation2042418
1
BBa_B0034
range2042418
1
55
66
annotation2042419
1
HIV-TAT
range2042419
1
73
108
annotation2042421
1
(LALAAAA)??3
range2042421
1
145
210
annotation2042422
1
BBa_B0034
range2042422
1
219
347
annotation2042417
1
T7(BBa_I712074)
range2042417
1
1
47
BBa_K210009_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggagaaatactagatgtatggacgtaaaaaacgtcgtggacgtcgtcgtggcggcggtcatcatcatcatcaccatggcggtggcctggccctggcggcggccgcgctggccctggcggcggccgcgctggccctggcggcggccgcgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z