BBa_K210011
1
BBa_K210011
LacI binding sites' tandem sequence #200
2009-10-14T11:00:00Z
2015-05-08T01:11:29Z
primer
This is a tandem sequence of lacI binding sites(GAAATGTGAGCGCTCACAACT). In the project "Time bomb" of iGEM Kyoto 2009, this part was used in order to stbilize linear DNA.
It is very difficult to clone this parts because tandem sequence can induce mutation. So, if you want to use this parts, you should cloning this under 32C under which it is liower possible that mutation happens.
false
false
_346_
0
5425
9
No part sequence
false
This parts was made by MPR.
Due to mutation, we could not suceed to sequence.
We confirmed this has only one set of EcoR1, Xba1, Spe1 and Pst1 recognition sites.
false
Makoto Kashima
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z