BBa_K2103000 1 BBa_K2103000 CYP2e1/NADPH P450 Reductase 2016-09-24T11:00:00Z 2016-09-29T09:45:36Z Human Co-expression of CYP2e1 gene (human) and NADPH P450 Reductase (electron transfer) for expression of the P450 cytochrome protein in E. Coli. true false _2571_ 26194 26194 9 false Added restriction sites for NdeI and HindIII as per previous pCW vector. false Maggie Chen component2484926 1 BBa_K1197014 component2484925 1 BBa_K799027 annotation2484925 1 BBa_K799027 range2484925 1 1 1501 annotation2484926 1 BBa_K1197014 range2484926 1 1508 3526 BBa_K1197014 1 BBa_K1197014 House fly cytochrome P450 reductase (fCPR) 2013-10-01T11:00:00Z 2015-05-08T01:09:39Z Its source is Musca domestica genome. fCPR is a cytochrome P450 reductase. It is necessary for functional working of CYP6G1. It makes CYP6G1 enzyme catalitically active. Reference: Andersen, J.F., Utermohlen, J.G., Feyereisen, R., 1994. Expression of house fly CYP6A1 and NADPH-cytochrome P450 reductase in Escherichia coli and reconstitution of an insecticide-metabolizing P450 system. Biochemistry 33, 2171e2177. false false _1511_ 0 12727 9 Not in stock false It is used as natural. Biobrick prefix and suffix were added by primers. false Alişan Kayab??len BBa_K799027 1 BBa_K799027 CYP2E1 2012-09-24T11:00:00Z 2015-05-08T01:13:24Z ... ... false false _1055_ 0 8447 9 It's complicated false Golden Gate Compatible. false Daniel Wolozny annotation2204780 1 CYP2E1 Gene range2204780 1 1 1501 BBa_K799027_sequence 1 ggtctcaaatgtctgctttgggtgttaccgttgctttgttggtttgggctgctttcttgttgttggtttctatgtggagacaagttcactcttcttggaacttgccaccaggtccattcccattgccaatcatcggtaacttgttccaattggaattgaagaacatcccaaagtctttcaccagattggctcaaagattcggtccagttttcaccttgtacgttggttctcaaagaatggttgttatgcacggttacaaggctgttaaggaagctttgttggactacaaggacgagttctctggtagaggtgacttgccagctttccacgctcacagagacagaggtatcatcttcaacaacggtccaacctggaaggacatcagaagattctctttgaccaccttgagaaactacggtatgggtaagcaaggtaacgaaagtagaatccaaagagaagctcacttcttgttggaagctttgagaaagacccaaggtcaaccattcgacccaaccttcttgatcggttgtgctccatgtaacgttatcgctgacatcttgttcagaaagcacttcgactacaacgacgaaaagttcttgagattgatgtacttgttcaacgaaaacttccacttgttgtctaccccatggttgcaattgtacaacaacttcccatctttcttgcactacttgccaggttctcacagaaaggttatcaagaacgttgctgaagttaaggaatacgtttctgaaagagttaaggaacaccaccaatctttggacccaaactgtccaagagacttgaccgactgtttgttggttgaaatggaaaaggaaaagcactctgctgaaagattgtacaccatggacggtatcaccgttaccgttgctgacttgttcttcgctggtaccgaaaccacctctaccaccttgagatacggtttgttgatcttgatgaagtacccagaaatcgaagaaaagttgcacgaagaaatcgacagagttatcggtccaagtagaatcccagctatcaaggacagacaagaaatgccatacatggacgctgttgttcacgaaatccaaagattcatcaccttggttccatctaacttgccacacgaagctaccagagacaccatcttcagaggttacttgatcccaaagggtaccgttgttgttccaaccttggactctgttttgtacgacaaccaagagttcccagacccagaaaagttcaagccagaacacttcttgaacgaaaacggtaagttcaagtactctgactacttcaagccattctctaccggtaagagagtttgtgctggtgaaggtttggctagaatggaattgttcttgttgttgtgtgctatcttgcaacacttcaacttgaagccattggttgacccaaaggacatcgacttgtctccaatccacatcggtttcggttgtatcccaccaagatacaagttgtgtgttatcccaagatcttgatgagcgagacc BBa_K2103000_sequence 1 ggtctcaaatgtctgctttgggtgttaccgttgctttgttggtttgggctgctttcttgttgttggtttctatgtggagacaagttcactcttcttggaacttgccaccaggtccattcccattgccaatcatcggtaacttgttccaattggaattgaagaacatcccaaagtctttcaccagattggctcaaagattcggtccagttttcaccttgtacgttggttctcaaagaatggttgttatgcacggttacaaggctgttaaggaagctttgttggactacaaggacgagttctctggtagaggtgacttgccagctttccacgctcacagagacagaggtatcatcttcaacaacggtccaacctggaaggacatcagaagattctctttgaccaccttgagaaactacggtatgggtaagcaaggtaacgaaagtagaatccaaagagaagctcacttcttgttggaagctttgagaaagacccaaggtcaaccattcgacccaaccttcttgatcggttgtgctccatgtaacgttatcgctgacatcttgttcagaaagcacttcgactacaacgacgaaaagttcttgagattgatgtacttgttcaacgaaaacttccacttgttgtctaccccatggttgcaattgtacaacaacttcccatctttcttgcactacttgccaggttctcacagaaaggttatcaagaacgttgctgaagttaaggaatacgtttctgaaagagttaaggaacaccaccaatctttggacccaaactgtccaagagacttgaccgactgtttgttggttgaaatggaaaaggaaaagcactctgctgaaagattgtacaccatggacggtatcaccgttaccgttgctgacttgttcttcgctggtaccgaaaccacctctaccaccttgagatacggtttgttgatcttgatgaagtacccagaaatcgaagaaaagttgcacgaagaaatcgacagagttatcggtccaagtagaatcccagctatcaaggacagacaagaaatgccatacatggacgctgttgttcacgaaatccaaagattcatcaccttggttccatctaacttgccacacgaagctaccagagacaccatcttcagaggttacttgatcccaaagggtaccgttgttgttccaaccttggactctgttttgtacgacaaccaagagttcccagacccagaaaagttcaagccagaacacttcttgaacgaaaacggtaagttcaagtactctgactacttcaagccattctctaccggtaagagagtttgtgctggtgaaggtttggctagaatggaattgttcttgttgttgtgtgctatcttgcaacacttcaacttgaagccattggttgacccaaaggacatcgacttgtctccaatccacatcggtttcggttgtatcccaccaagatacaagttgtgtgttatcccaagatcttgatgagcgagacctactagatggctgcggaacacgtcgaagaagtggtttcggaagaaccatttttgggcacactggatattgcattattagtagtcctcttagtcggtgcaacatggtactttatgagaagtcgtaagaaggaagaagctccaattcgttcatattctatacaacccactacagtcagcaccgtttcaacgacagagaactcgttcataaaaaaattgaaagcttcaggtcgtagtttagttgtgttttatggttcccagaccggaacagccgaagaatttgccggacgattggccaaggaaggtttacgctatcgcatgaagggaatggtggcggaccccgaagaatgtgacatggaagaattgttacaaatgaaggatattcctaactcattagccgttttctgtttggctacatatggtgaaggagatcccactgataacgctatggaattttatgaatggattacaaatggcgaggttgatttgacgggtttaaactacgccgtatttggcttaggcaacaagacttatgaacattacaacaaagttgcaatttatgtggacaagaggttggaggaactcggtgctacgagagtgttcgagttaggtcttggagacgatgatgcaaatatcgaagatgacttcattacgtggaaagacagattttggccatctgtttgtgatttcttcggaatcgaaggaagtggtgaggaggtattgatgcgtcaattccgacttttggaacagccggatgttcaacccgatcgtatttacaccggcgaaatagctcggttgcactcaatgcaaaatcaaagacctccatttgatgctaaaaatccatttttggcttcagtcatagttaatcgagagctgcataaaggcggcggacggtcatgtatgcatattgagttggatattgatggatcaaaaatgcggtatgatgcaggcgaccatatagcaatgtatccgattaatgataaaatcctggtagaaaaattgggtaaactatgtgatgcaaatctggatacagtcttctctttgatcaatacggatacggatagcagtaaaaaacatccattcccctgtcccaccacataccgtaccgccctgacacattacttggagattactgcaataccgagaacacatattctgaaggagttagcagagtattgttccgatgaaaaggataaggaatttttacgcaacatggcgtctattactccggaaggcaaagagaagtatcagaattggatccagaactcttcaagaaatattgtgcatattttggaggatatcaaatcttgtagaccaccaattgatcatatttgtgaactgttgccaagacttcaaccgcgttattactcgatttcatcttcttctaagctctatccaactaatgttcatattacagcagttctggttcaatatgaaacacctactggccgtgtaaacaaaggtgttgctacgtcatacatgaaggaaaaaaatccttcggttggagaggttaaagtgcctgtatttattcgcaaatctcaatttaggttgccaactaaatccgagattccaatcataatggtgggcccaggaacgggtctagcacctttcagaggattcatacaggaaagacaatttttacgagatggaggaaaagttgtcggcgataccatattgtatttcgggtgtagaaagaaggacgaagacttcatttatagagaagaactagagcaatatgtacaaaatggaactttaaccttgaaaacggcattttcaagagaccaacaagaaaaaatttatgttactcatctaatcgaacaagatgctgatttaatttggaaagtaattggcgaacaaaagggacacttttatatttgcggggatgctaaaaatatggctgtggatgttagaaatattttagttaaaattttgtcaaccaaaggcaatatgaacgaatcagatgctgtgcaatatataaaaaagatggaggcacaaaagcgttattccgcggacgtttggagctaataa BBa_K1197014_sequence 1 atggctgcggaacacgtcgaagaagtggtttcggaagaaccatttttgggcacactggatattgcattattagtagtcctcttagtcggtgcaacatggtactttatgagaagtcgtaagaaggaagaagctccaattcgttcatattctatacaacccactacagtcagcaccgtttcaacgacagagaactcgttcataaaaaaattgaaagcttcaggtcgtagtttagttgtgttttatggttcccagaccggaacagccgaagaatttgccggacgattggccaaggaaggtttacgctatcgcatgaagggaatggtggcggaccccgaagaatgtgacatggaagaattgttacaaatgaaggatattcctaactcattagccgttttctgtttggctacatatggtgaaggagatcccactgataacgctatggaattttatgaatggattacaaatggcgaggttgatttgacgggtttaaactacgccgtatttggcttaggcaacaagacttatgaacattacaacaaagttgcaatttatgtggacaagaggttggaggaactcggtgctacgagagtgttcgagttaggtcttggagacgatgatgcaaatatcgaagatgacttcattacgtggaaagacagattttggccatctgtttgtgatttcttcggaatcgaaggaagtggtgaggaggtattgatgcgtcaattccgacttttggaacagccggatgttcaacccgatcgtatttacaccggcgaaatagctcggttgcactcaatgcaaaatcaaagacctccatttgatgctaaaaatccatttttggcttcagtcatagttaatcgagagctgcataaaggcggcggacggtcatgtatgcatattgagttggatattgatggatcaaaaatgcggtatgatgcaggcgaccatatagcaatgtatccgattaatgataaaatcctggtagaaaaattgggtaaactatgtgatgcaaatctggatacagtcttctctttgatcaatacggatacggatagcagtaaaaaacatccattcccctgtcccaccacataccgtaccgccctgacacattacttggagattactgcaataccgagaacacatattctgaaggagttagcagagtattgttccgatgaaaaggataaggaatttttacgcaacatggcgtctattactccggaaggcaaagagaagtatcagaattggatccagaactcttcaagaaatattgtgcatattttggaggatatcaaatcttgtagaccaccaattgatcatatttgtgaactgttgccaagacttcaaccgcgttattactcgatttcatcttcttctaagctctatccaactaatgttcatattacagcagttctggttcaatatgaaacacctactggccgtgtaaacaaaggtgttgctacgtcatacatgaaggaaaaaaatccttcggttggagaggttaaagtgcctgtatttattcgcaaatctcaatttaggttgccaactaaatccgagattccaatcataatggtgggcccaggaacgggtctagcacctttcagaggattcatacaggaaagacaatttttacgagatggaggaaaagttgtcggcgataccatattgtatttcgggtgtagaaagaaggacgaagacttcatttatagagaagaactagagcaatatgtacaaaatggaactttaaccttgaaaacggcattttcaagagaccaacaagaaaaaatttatgttactcatctaatcgaacaagatgctgatttaatttggaaagtaattggcgaacaaaagggacacttttatatttgcggggatgctaaaaatatggctgtggatgttagaaatattttagttaaaattttgtcaaccaaaggcaatatgaacgaatcagatgctgtgcaatatataaaaaagatggaggcacaaaagcgttattccgcggacgtttggagctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z