BBa_K2109108 1 BBa_K2109108 MS2 6XHis (V29I/d1FG) 2016-10-13T11:00:00Z 2016-10-28T01:07:17Z n/a this part is an improvement upon BBA_K1791000 from the Lethbridge iGEM 2015 submission. It contains a N terminal His tag on a modified MS2 coat protein sequence. The sequence has been codon optimized for Ecoli and modified via a V29I mutation and a 13 aa deletion with a Gly/Ala insertion. These mutations prevent capsid formation as well as dimerization of MS2 for more efficient yields during purification. false false _2216_2577_ 17031 17031 9 false n/a false Andy Hudson BBa_K2109108_sequence 1 taatacgactcactataggggaattgtgagcggataacaattccccactagccatgattctgtcaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggcagccatatggcgtcgaactttactcaattcgtactggttgataacggggggacgggcgacgtaactgtggctccttctaactttgctaatggcatcgccgaatggatctcgagcaactcgcgctcacaagcgtacaaagtaacgtgctctgttcgtcagtcgagcgcgcagaatcggaagtacaccattaaagtggaagtaccgaaaggcgcctggcgctcttatttgaacatggaactcacaatcccaatctttgcaaccaattcagattgtgaacttatcgtgaaagccatgcaaggtctgctgaaagatgggaacccgattccgagtgcaattgcggccaatagtggcatttactgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z