BBa_K2110006 1 BBa_K2110006 TPA regulatory gene expression system 2016-10-05T11:00:00Z 2016-10-06T01:20:49Z BBa_K2110012 BBa_K2110002 BBa_K2110000 This part consists of the enhanced promoter PGK1(BBa_K2110012), the tpaR gene(BBa_K2110002), and the terminator CYC1（BBa_K2110000）. The tpaR gene encodes the terephthalic acid (TPA) positive feedback regulatory protein. This protein can bind the TPA inducible leader sequence (TILS) at the existence of TPA and induce the expression of downstream gene. The tpaR protein belongs to the IclR protein family and has helix-turn-helix domain. false false _2578_ 32957 32957 9 false / false Le He component2486542 1 BBa_K2110002 component2486540 1 BBa_K2110012 component2486543 1 BBa_K2110000 annotation2486542 1 BBa_K2110002 range2486542 1 788 1594 annotation2486540 1 BBa_K2110012 range2486540 1 1 781 annotation2486543 1 BBa_K2110000 range2486543 1 1603 1850 BBa_K2110000 1 BBa_K2110000 CYC1 Terminator 2016-10-03T11:00:00Z 2016-10-04T10:55:00Z It comes from genome sequence of Saccharomyces cerevisiae CYC1 Terminator is widely used in Saccharomyces cerevisiae. It is a composite part in many vectors of Saccharomyces cerevisiae like plasmid pYES2. Amplifying it by PCR and link it after your protein coding sequence is also feasible if you want to express the gene in Saccharomyces cerevisiae. false false _2578_ 32964 32964 9 false There is no neccessary consideration in detailed design of the sequence false Haoran Ding annotation2485997 1 CYC1 Terminator range2485997 1 1 1 BBa_K2110002 1 BBa_K2110002 TPA positive feedback regulatory gene. 2016-10-04T11:00:00Z 2016-10-05T11:22:33Z It comes from the genome of RhodococcusjostiiRHA1. This gene encodes the terephthalic acid(TPA) transporting protein. TPA is not a kind of nutrient substance for most organisms, including common chassis organisms like E.coli and Saccharomyces cerevisiae. However, as a kind of TPA degradable organism, RhodococcusjostiiRHA1 can transport TPA into their cells because of the TPA transporting protein genetpaK in their genome. This gene is also verified to be useful in Saccharomyces cerevisiae. false false _2578_ 32957 32957 9 false / false Le He annotation2486177 1 BioBrick range2486177 1 1 807 BBa_K2110012 1 BBa_K2110012 PGK1 promoter 2016-10-05T11:00:00Z 2016-10-06T12:43:19Z Saccharomyces cerevisiae This promoter is a enhanced promoter used in Saccharomyces cerevisiae. It regulates the 3-phosphoglycerate kinase (PGK1) gene in Saccharomyces cerevisiae. This promoter can be used in both plasmid and genome of Saccharomyces cerevisiae. false false _2578_ 32957 32957 9 false / false Le He annotation2486527 1 promoter range2486527 1 1 781 BBa_K2110012_sequence 1 tattttagattcctgacttcaactcaagacgcacagatattataacatctgcacaataggcatttgcaagaattactcgtgagtaaggaaagagtgaggaactatcgcatacctgcatttaaagatgccgatttgggcgcgaatcctttattttggcttcaccctcatactattatcagggccagaaaaaggaagtgtttccctccttcttgaattgatgttaccctcataaagcacgtggcctcttatcgagaaagaaattaccgtcgctcgtgatttgtttgcaaaaagaacaaaactgaaaaaacccagacacgctcgacttcctgtcttcctattgattgcagcttccaatttcgtcacacaacaaggtcctagcgacggctcacaggttttgtaacaagcaatcgaaggttctggaatggcgggaaagggtttagtaccacatgctatgatgcccactgtgatctccagagcaaagttcgttcgatcgtactgttactctctctctttcaaacagaattgtccgaatcgtgtgacaacaacagcctgttctcacacactcttttcttctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagttttttcaagttcttagatgctttctttttctctttttttacagatcatcaaggaagtaattatctactttttacaacaaatataaaaaca BBa_K2110006_sequence 1 tattttagattcctgacttcaactcaagacgcacagatattataacatctgcacaataggcatttgcaagaattactcgtgagtaaggaaagagtgaggaactatcgcatacctgcatttaaagatgccgatttgggcgcgaatcctttattttggcttcaccctcatactattatcagggccagaaaaaggaagtgtttccctccttcttgaattgatgttaccctcataaagcacgtggcctcttatcgagaaagaaattaccgtcgctcgtgatttgtttgcaaaaagaacaaaactgaaaaaacccagacacgctcgacttcctgtcttcctattgattgcagcttccaatttcgtcacacaacaaggtcctagcgacggctcacaggttttgtaacaagcaatcgaaggttctggaatggcgggaaagggtttagtaccacatgctatgatgcccactgtgatctccagagcaaagttcgttcgatcgtactgttactctctctctttcaaacagaattgtccgaatcgtgtgacaacaacagcctgttctcacacactcttttcttctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagttttttcaagttcttagatgctttctttttctctttttttacagatcatcaaggaagtaattatctactttttacaacaaatataaaaacatactagatgagcaattctcgaacaattaagccggacgataagggaagtgtcaagggtgctgcccgcgctcccgagggaatggccggactggcgaagggcctggcggtcatcgaggccttcggcatccgccacccgcaactgaccgtcaccgacgccgctcagcggacgaacacctcccgcgccgcggcgcgccgctgcctgctcactctcgtcgagctcggttacctgacccacgacaacggaaacttccgaccgacacccaggatgcttcggctgggcggggcctatctggacaccgcgccactgccgcaacttgcccagccgcagttggtcgaggcccgcgatgcgctgggcgagtcggtgtcgctcgcggttctcgaagacgggtgttccctgttcgtcgcccgcgccgaggccgcacggatcgtcgcgaccggagtccgggtcggcgccaaactgcccgcccacagctccgcgaccggtcgagtactgctggccggacttcccgagacgcaactcgacgactacctccgagaatgccacccgcagccgcgcacacccaagacactgaccgatgtcgatgccatcagcgagcgaatcctgaccgcgagggagagcggcgtggcctgcaccgacgaagaaatcgaactcggcatgctgtcgatagccgtaccggtgcggactgcccggggcgaaaccgtggcggcaatgagtgtcagtgcgtccacagccagaaccaccctgcacgacatccgtgaggcagccgtgcccgtactgaacaagtacgcagcaagactcggccggcttctgtgatactagaggcaaattaaagccttcgagcgtcccaaaaccttctcaagcaaggttttcagtataatgttacatgcgtacacgcgtctgtacagaaaaaaaagaaaaatttgaaatataaataacgttcttaatactaacataactataaaaaaataaatagggacctagacttcaggttgtctaactccttccttttcggttagagcggatgtggggggagggcgtgaatgtaagcgtgacataactaattacatga BBa_K2110002_sequence 1 atgagcaattctcgaacaattaagccggacgataagggaagtgtcaagggtgctgcccgcgctcccgagggaatggccggactggcgaagggcctggcggtcatcgaggccttcggcatccgccacccgcaactgaccgtcaccgacgccgctcagcggacgaacacctcccgcgccgcggcgcgccgctgcctgctcactctcgtcgagctcggttacctgacccacgacaacggaaacttccgaccgacacccaggatgcttcggctgggcggggcctatctggacaccgcgccactgccgcaacttgcccagccgcagttggtcgaggcccgcgatgcgctgggcgagtcggtgtcgctcgcggttctcgaagacgggtgttccctgttcgtcgcccgcgccgaggccgcacggatcgtcgcgaccggagtccgggtcggcgccaaactgcccgcccacagctccgcgaccggtcgagtactgctggccggacttcccgagacgcaactcgacgactacctccgagaatgccacccgcagccgcgcacacccaagacactgaccgatgtcgatgccatcagcgagcgaatcctgaccgcgagggagagcggcgtggcctgcaccgacgaagaaatcgaactcggcatgctgtcgatagccgtaccggtgcggactgcccggggcgaaaccgtggcggcaatgagtgtcagtgcgtccacagccagaaccaccctgcacgacatccgtgaggcagccgtgcccgtactgaacaagtacgcagcaagactcggccggcttctgtga BBa_K2110000_sequence 1 gcaaattaaagccttcgagcgtcccaaaaccttctcaagcaaggttttcagtataatgttacatgcgtacacgcgtctgtacagaaaaaaaagaaaaatttgaaatataaataacgttcttaatactaacataactataaaaaaataaatagggacctagacttcaggttgtctaactccttccttttcggttagagcggatgtggggggagggcgtgaatgtaagcgtgacataactaattacatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z