BBa_K2110014
1
BBa_K2110014
MHETase enzyme after codon optimization for bacillus subtilis
2016-10-05T11:00:00Z
2016-10-06T02:34:30Z
It comes from the sequence synthesis.
This gene encodes the MHETase enzyme. This protein can bind on the PET surface and degrade MHET to TPA and EG, which have higher efficiency than other cutinase.
This enzyme cooperates with PETase enzyme well in PET degradation. At first, this gene couldn???t fully express in bacillus subtilis. So we use codon optimization for bacillus subtilis for better expression and secretion.
false
false
_2578_
30842
32957
9
false
/
false
Le He
BBa_K2110014_sequence
1
atgaaatttgtaaaaagaaggatcattgcacttgtaacaattttgatgctgtctgttacatcgctgtttgcgttgcagccgtcagcaaaagccggcggaggatcaacacctttaccgctgcctcaacaacaaccgccgcaacaagaacctcctccgcctcctgttcctctggcatcaagagcagcatgcgaagcactgaaagatggcaatggcgatatggtttggccgaatgcagcaacagttgttgaagttgcagcatggagagatgcagcaccggcaacagcatcagcagcagcactgccggaacattgcgaagtttcaggcgcaattgcaaaaagaacaggcattgatggctatccgtacgagattaagtttagactgagaatgccggcagaatggaatggccgcttttttatggaaggcggctcaggcacaaatggctcactgtcagcagcaacaggctcaattggcggcggccaaattgcatcagcactgtcaagaaactttgcgacgattgcaacagatggcggccatgataatgcagttaacgataatccggatgcactgggcacagttgcatttggcctggacccgcaagcaagactggatatgggctataacagctatgaccaggttacacaagcaggcaaagcagcagttgcaagattttatggcagagcagcagataagtcatactttattggctgctcagaaggcggaagagaaggcatgatgctgtcacaaagatttccgtcacattacgatggcattgttgcaggcgcaccgggatatcaacttccgaaagcaggcatttcaggcgcatggacaacacaatcactggcaccggcagcagttggcttagatgcacaaggcgttccgctgattaataagtcattcagcgacgcagacctgcatctgctgtcacaagcaattctgggcacatgcgatgcacttgatggcctggcagatggcattgtggataattatagagcatgccaggcagcatttgatccggcaacagcagcaaatccggcaaatggccaagcactgcaatgcgttggcgcaaaaacagcagattgcctgtcaccggttcaagttacggcaatcaaaagagcaatggcaggcccggttaattcagcaggcacaccgctgtataatagatgggcatgggatgcaggaatgtcaggcctgtcaggcacaacatataatcaaggctggagatcatggtggctgggctcatttaattcatcagcgaacaatgcacaaagagtgtcaggcttttcagcaagatcatggctggttgattttgcaacaccgccggaacctatgccgatgacacaagttgcagcgagaatgatgaagttcgatttcgatatcgacccgctgaaaatttgggcaacaagcggccaatttacacaatcaagcatggattggcatggcgcaacatcaacggatctggcagcatttagagatagaggcggcaaaatgattctgtatcacggcatgtcagatgcagcattttcagcactggatacagcagattactatgaaagactgggcgcagcaatgcctggagcagcaggatttgcaagactgtttctggttccgggcatgaatcattgttcaggcggccctggcacagatagatttgatatgctgacaccgctggttgcatgggttgaaagaggcgaagcaccggatcaaatttcagcatggtcaggcacaccgggctattttggcgttgcagcaagaacaagaccgctgtgcccgtatcctcaaattgcacgctataaaggctcaggcgacattaatacggaagcgaactttgcatgcgcagcacctccgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z