BBa_K2110102 1 BBa_K2110102 PETase site-directed mutant M154L 2016-10-11T11:00:00Z 2016-10-16T01:48:23Z It comes from the genome of Saccharomyces cerevisiae This is one of the site-directed mutant of the PETase gene, we change the 154th amino acid from M to L. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant. false false _2578_ 32964 32964 9 false It should be expressed in Saccharomyces cerevisiae false Haoran Ding annotation2493826 1 misc range2493826 1 1 795 BBa_K2110102_sequence 1 atgcaaacaaatccatatgcaagaggtccaaatccaactgcggcttcattagaggcttctgcgggtccttttactgttagatcttttactgtttcaagaccatctggttatggtgcaggtacagtttattacccaactaatgctggtggtacagttggtgctattgcaattgttccaggttacacagcaagacaatcttcaattaaatggtggggtccaagattggcttcacatggtttcgttgttattactatcgatacaaactctactttggatcaaccatcttcaagatcttcacaacaaatggcagctttgagacaagttgcatctttaaatggtacttcttcatctccaatctatggtaaagttgatacagctagactgggtgttatgggttggtcaatgggtggtggtggttcattgatctctgcggctaacaacccatctttgaaggcagctgcaccacaagctccatgggattcatctactaacttctcatctgttacagttccaactttgatcttcgcttgtgaaaacgattctatcgctccagttaactcatctgcattgccaatctatgattcaatgtctaggaacgctaagcaattcttggaaattaatggtggttcacattcttgtgctaactctggtaactctaaccaagcattgatcggtaaaaagggtgttgcttggatgaagagattcatggataacgatacaagatactcaactttcgcatgtgaaaacccaaactcaactagagtttctgatttcagaacagctaattgttcttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z