BBa_K2110107 1 BBa_K2110107 PETase site-directed mutant L117F 2016-10-11T11:00:00Z 2016-10-16T01:51:50Z It is derived from the part BBa_K2110800. This is one of the site-directed mutant of the PETase gene, we change the 117th amino acid from L to F. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant. false false _2578_ 32964 32964 9 false It should be expressed in Saccharomyces cerevisiae. false Haoran Ding annotation2493831 1 misc range2493831 1 1 795 BBa_K2110107_sequence 1 atgcaaacaaatccatatgcaagaggtccaaatccaactgcggcttcattagaggcttctgcgggtccttttactgttagatcttttactgtttcaagaccatctggttatggtgcaggtacagtttattacccaactaatgctggtggtacagttggtgctattgcaattgttccaggttacacagcaagacaatcttcaattaaatggtggggtccaagattggcttcacatggtttcgttgttattactatcgatacaaactctactttcgatcaaccatcttcaagatcttcacaacaaatggcagctttgagacaagttgcatctttaaatggtacttcttcatctccaatctatggtaaagttgatacagctagaatgggtgttatgggttggtcaatgggtggtggtggttcattgatctctgcggctaacaacccatctttgaaggcagctgcaccacaagctccatgggattcatctactaacttctcatctgttacagttccaactttgatcttcgcttgtgaaaacgattctatcgctccagttaactcatctgcattgccaatctatgattcaatgtctaggaacgctaagcaattcttggaaattaatggtggttcacattcttgtgctaactctggtaactctaaccaagcattgatcggtaaaaagggtgttgcttggatgaagagattcatggataacgatacaagatactcaactttcgcatgtgaaaacccaaactcaactagagtttctgatttcagaacagctaattgttcttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z