LOCUS BBa_K2110200 2113 bp DNA linear 26-Nov-2024 DEFINITION PETase expression system in Saccharomyces cerevisiae ACCESSION BBa_K2110200 VERSION BBa_K2110200.1 FEATURES Location/Qualifiers promoter 1..781 /label=promoter promoter 1..781 /label=BBa_K2110012 misc_feature 790..1056 /label=misc misc_feature 790..1056 /label=BBa_K2110017 misc_feature 1063..1857 /label=misc CDS 1063..1857 /label=BBa_K2110800 misc_feature 1866..1866 /label=CYC1 Terminator terminator 1866..2113 /label=BBa_K2110000 ORIGIN 1 tattttagat tcctgacttc aactcaagac gcacagatat tataacatct gcacaatagg 61 catttgcaag aattactcgt gagtaaggaa agagtgagga actatcgcat acctgcattt 121 aaagatgccg atttgggcgc gaatccttta ttttggcttc accctcatac tattatcagg 181 gccagaaaaa ggaagtgttt ccctccttct tgaattgatg ttaccctcat aaagcacgtg 241 gcctcttatc gagaaagaaa ttaccgtcgc tcgtgatttg tttgcaaaaa gaacaaaact 301 gaaaaaaccc agacacgctc gacttcctgt cttcctattg attgcagctt ccaatttcgt 361 cacacaacaa ggtcctagcg acggctcaca ggttttgtaa caagcaatcg aaggttctgg 421 aatggcggga aagggtttag taccacatgc tatgatgccc actgtgatct ccagagcaaa 481 gttcgttcga tcgtactgtt actctctctc tttcaaacag aattgtccga atcgtgtgac 541 aacaacagcc tgttctcaca cactcttttc ttctaaccaa gggggtggtt tagtttagta 601 gaacctcgtg aaacttacat ttacatatat ataaacttgc ataaattggt caatgcaaga 661 aatacatatt tggtcttttc taattcgtag ttttttcaag ttcttagatg ctttcttttt 721 ctcttttttt acagatcatc aaggaagtaa ttatctactt tttacaacaa atataaaaac 781 atactagaga gcttcagcct ctcttttatc caaagatacc ccttcttctt tagcagcaat 841 gctggcaata gtagtattta taaacaataa cccgttattt gtgctgttgg aaaatggcaa 901 aacagcaaca tcgaaatccc cttctaaatc caagtaaccg atgacagctt cagccggaat 961 ttgtgcggtt tcatcttctg ttgtagtgtt gactggagca gctaatgcgg aggatgctgc 1021 gaataaaacg gcagtaaaaa ttgaaggaaa tctcattact agatgcaaac aaatccatat 1081 gcaagaggtc caaatccaac tgcggcttca ttagaggctt ctgcgggtcc ttttactgtt 1141 agatctttta ctgtttcaag accatctggt tatggtgcag gtacagttta ttacccaact 1201 aatgctggtg gtacagttgg tgctattgca attgttccag gttacacagc aagacaatct 1261 tcaattaaat ggtggggtcc aagattggct tcacatggtt tcgttgttat tactatcgat 1321 acaaactcta ctttggatca accatcttca agatcttcac aacaaatggc agctttgaga 1381 caagttgcat ctttaaatgg tacttcttca tctccaatct atggtaaagt tgatacagct 1441 agaatgggtg ttatgggttg gtcaatgggt ggtggtggtt cattgatctc tgcggctaac 1501 aacccatctt tgaaggcagc tgcaccacaa gctccatggg attcatctac taacttctca 1561 tctgttacag ttccaacttt gatcttcgct tgtgaaaacg attctatcgc tccagttaac 1621 tcatctgcat tgccaatcta tgattcaatg tctaggaacg ctaagcaatt cttggaaatt 1681 aatggtggtt cacattcttg tgctaactct ggtaactcta accaagcatt gatcggtaaa 1741 aagggtgttg cttggatgaa gagattcatg gataacgata caagatactc aactttcgca 1801 tgtgaaaacc caaactcaac tagagtttct gatttcagaa cagctaattg ttcttaatac 1861 tagaggcaaa ttaaagcctt cgagcgtccc aaaaccttct caagcaaggt tttcagtata 1921 atgttacatg cgtacacgcg tctgtacaga aaaaaaagaa aaatttgaaa tataaataac 1981 gttcttaata ctaacataac tataaaaaaa taaataggga cctagacttc aggttgtcta 2041 actccttcct tttcggttag agcggatgtg gggggagggc gtgaatgtaa gcgtgacata 2101 actaattaca tga //