LOCUS       BBa_K2110200            2113 bp    DNA     linear       27-Dec-2024
DEFINITION  PETase expression system in Saccharomyces cerevisiae
ACCESSION   BBa_K2110200
VERSION     BBa_K2110200.1  
FEATURES             Location/Qualifiers
     promoter        1..781
                     /label=promoter
     promoter        1..781
                     /label=BBa_K2110012
     misc_feature    790..1056
                     /label=misc
     misc_feature    790..1056
                     /label=BBa_K2110017
     misc_feature    1063..1857
                     /label=misc
     CDS             1063..1857
                     /label=BBa_K2110800
     misc_feature    1866..1866
                     /label=CYC1 Terminator
     terminator      1866..2113
                     /label=BBa_K2110000
ORIGIN
        1 tattttagat tcctgacttc aactcaagac gcacagatat tataacatct gcacaatagg
       61 catttgcaag aattactcgt gagtaaggaa agagtgagga actatcgcat acctgcattt
      121 aaagatgccg atttgggcgc gaatccttta ttttggcttc accctcatac tattatcagg
      181 gccagaaaaa ggaagtgttt ccctccttct tgaattgatg ttaccctcat aaagcacgtg
      241 gcctcttatc gagaaagaaa ttaccgtcgc tcgtgatttg tttgcaaaaa gaacaaaact
      301 gaaaaaaccc agacacgctc gacttcctgt cttcctattg attgcagctt ccaatttcgt
      361 cacacaacaa ggtcctagcg acggctcaca ggttttgtaa caagcaatcg aaggttctgg
      421 aatggcggga aagggtttag taccacatgc tatgatgccc actgtgatct ccagagcaaa
      481 gttcgttcga tcgtactgtt actctctctc tttcaaacag aattgtccga atcgtgtgac
      541 aacaacagcc tgttctcaca cactcttttc ttctaaccaa gggggtggtt tagtttagta
      601 gaacctcgtg aaacttacat ttacatatat ataaacttgc ataaattggt caatgcaaga
      661 aatacatatt tggtcttttc taattcgtag ttttttcaag ttcttagatg ctttcttttt
      721 ctcttttttt acagatcatc aaggaagtaa ttatctactt tttacaacaa atataaaaac
      781 atactagaga gcttcagcct ctcttttatc caaagatacc ccttcttctt tagcagcaat
      841 gctggcaata gtagtattta taaacaataa cccgttattt gtgctgttgg aaaatggcaa
      901 aacagcaaca tcgaaatccc cttctaaatc caagtaaccg atgacagctt cagccggaat
      961 ttgtgcggtt tcatcttctg ttgtagtgtt gactggagca gctaatgcgg aggatgctgc
     1021 gaataaaacg gcagtaaaaa ttgaaggaaa tctcattact agatgcaaac aaatccatat
     1081 gcaagaggtc caaatccaac tgcggcttca ttagaggctt ctgcgggtcc ttttactgtt
     1141 agatctttta ctgtttcaag accatctggt tatggtgcag gtacagttta ttacccaact
     1201 aatgctggtg gtacagttgg tgctattgca attgttccag gttacacagc aagacaatct
     1261 tcaattaaat ggtggggtcc aagattggct tcacatggtt tcgttgttat tactatcgat
     1321 acaaactcta ctttggatca accatcttca agatcttcac aacaaatggc agctttgaga
     1381 caagttgcat ctttaaatgg tacttcttca tctccaatct atggtaaagt tgatacagct
     1441 agaatgggtg ttatgggttg gtcaatgggt ggtggtggtt cattgatctc tgcggctaac
     1501 aacccatctt tgaaggcagc tgcaccacaa gctccatggg attcatctac taacttctca
     1561 tctgttacag ttccaacttt gatcttcgct tgtgaaaacg attctatcgc tccagttaac
     1621 tcatctgcat tgccaatcta tgattcaatg tctaggaacg ctaagcaatt cttggaaatt
     1681 aatggtggtt cacattcttg tgctaactct ggtaactcta accaagcatt gatcggtaaa
     1741 aagggtgttg cttggatgaa gagattcatg gataacgata caagatactc aactttcgca
     1801 tgtgaaaacc caaactcaac tagagtttct gatttcagaa cagctaattg ttcttaatac
     1861 tagaggcaaa ttaaagcctt cgagcgtccc aaaaccttct caagcaaggt tttcagtata
     1921 atgttacatg cgtacacgcg tctgtacaga aaaaaaagaa aaatttgaaa tataaataac
     1981 gttcttaata ctaacataac tataaaaaaa taaataggga cctagacttc aggttgtcta
     2041 actccttcct tttcggttag agcggatgtg gggggagggc gtgaatgtaa gcgtgacata
     2101 actaattaca tga
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