BBa_K2110012 1 BBa_K2110012 PGK1 promoter 2016-10-05T11:00:00Z 2016-10-06T12:43:19Z Saccharomyces cerevisiae This promoter is a enhanced promoter used in Saccharomyces cerevisiae. It regulates the 3-phosphoglycerate kinase (PGK1) gene in Saccharomyces cerevisiae. This promoter can be used in both plasmid and genome of Saccharomyces cerevisiae. false false _2578_ 32957 32957 9 false / false Le He annotation2486527 1 promoter range2486527 1 1 781 BBa_K2110200 1 BBa_K2110200 PETase expression system in Saccharomyces cerevisiae 2016-10-12T11:00:00Z 2016-11-16T07:21:34Z BBa_K2110012, BBa_K2110017, BBa_K2110800 or BBa_K2110100-BBa_K2110109, BBa_K2110001 Saccharomyces cerevisiae is a widely used chassis organism in synthetic biology research. We use it as the chassis organism to express the PETase gene and assay the enzyme activity. This composite part consists of the enhanced promoter PGK1 (BBa_K2110012), α signal peptide (BBa_K2110017), PETase gene (or its various mutants, BBa_K2110800, BBa_K2110100-BBa_K2110109), and CYC1 terminator (BBa_K2110001). The expression data can be accessed respectively in the part information page of PETase gene and its mutants. (BBa_K2110800, BBa_K2110100-BBa_K2110109). false false _2578_ 32964 32964 9 It's complicated false It should be used in Saccharomyces cerevisiae. false Haoran Ding component2531638 1 BBa_K2110800 component2531639 1 BBa_K2110000 component2531634 1 BBa_K2110012 component2531636 1 BBa_K2110017 annotation2531638 1 BBa_K2110800 range2531638 1 1063 1857 annotation2531634 1 BBa_K2110012 range2531634 1 1 781 annotation2531639 1 BBa_K2110000 range2531639 1 1866 2113 annotation2531636 1 BBa_K2110017 range2531636 1 790 1056 BBa_K2110000 1 BBa_K2110000 CYC1 Terminator 2016-10-03T11:00:00Z 2016-10-04T10:55:00Z It comes from genome sequence of Saccharomyces cerevisiae CYC1 Terminator is widely used in Saccharomyces cerevisiae. It is a composite part in many vectors of Saccharomyces cerevisiae like plasmid pYES2. Amplifying it by PCR and link it after your protein coding sequence is also feasible if you want to express the gene in Saccharomyces cerevisiae. false false _2578_ 32964 32964 9 false There is no neccessary consideration in detailed design of the sequence false Haoran Ding annotation2485997 1 CYC1 Terminator range2485997 1 1 1 BBa_K2110017 1 BBa_K2110017 α-factor signal peptide 2016-10-05T11:00:00Z 2016-10-13T02:37:36Z Genome of Saccharomyces cerevisiae This sequence encodes the α-factor signal peptide. This peptide helps the secretion of α-factor of Saccharomyces cerevisiae. Attaching it before the protein can realize the secretion of protein in Saccharomyces cerevisiae. false false _2578_ 32964 32957 9 false / false Le He annotation2496602 1 misc range2496602 1 1 267 BBa_K2110800 1 BBa_K2110800 PETase 2016-10-04T11:00:00Z 2016-10-06T12:55:00Z Come from the genome of Ideonella sakaiensis (strain 201-F6) The sequence codes the wild type protein called PETase. PETase is a Poly(ethylene terephthalate) hydrolase, which was found in Ideonella sakaiensis (strain 201-F6) by Japanese scientists. It allows Ideonella sakaiensis to degrade PET. If properly transferred into engineered organisms as E.Coli or yeast, secreted PETase can catalyze the hydrolysis of PET to produce mono(2-hydroxyethyl) terephthalate (MHET) as the major product. Optimum temperature is 40 degrees Celsius for PET film hydrolysis and optimum pH is 9. false false _2578_ 32964 32965 9 false This enzyme should be work in extracellular environments. false Haipeng Yu annotation2486182 1 misc range2486182 1 1 795 BBa_K2110012_sequence 1 tattttagattcctgacttcaactcaagacgcacagatattataacatctgcacaataggcatttgcaagaattactcgtgagtaaggaaagagtgaggaactatcgcatacctgcatttaaagatgccgatttgggcgcgaatcctttattttggcttcaccctcatactattatcagggccagaaaaaggaagtgtttccctccttcttgaattgatgttaccctcataaagcacgtggcctcttatcgagaaagaaattaccgtcgctcgtgatttgtttgcaaaaagaacaaaactgaaaaaacccagacacgctcgacttcctgtcttcctattgattgcagcttccaatttcgtcacacaacaaggtcctagcgacggctcacaggttttgtaacaagcaatcgaaggttctggaatggcgggaaagggtttagtaccacatgctatgatgcccactgtgatctccagagcaaagttcgttcgatcgtactgttactctctctctttcaaacagaattgtccgaatcgtgtgacaacaacagcctgttctcacacactcttttcttctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagttttttcaagttcttagatgctttctttttctctttttttacagatcatcaaggaagtaattatctactttttacaacaaatataaaaaca BBa_K2110200_sequence 1 tattttagattcctgacttcaactcaagacgcacagatattataacatctgcacaataggcatttgcaagaattactcgtgagtaaggaaagagtgaggaactatcgcatacctgcatttaaagatgccgatttgggcgcgaatcctttattttggcttcaccctcatactattatcagggccagaaaaaggaagtgtttccctccttcttgaattgatgttaccctcataaagcacgtggcctcttatcgagaaagaaattaccgtcgctcgtgatttgtttgcaaaaagaacaaaactgaaaaaacccagacacgctcgacttcctgtcttcctattgattgcagcttccaatttcgtcacacaacaaggtcctagcgacggctcacaggttttgtaacaagcaatcgaaggttctggaatggcgggaaagggtttagtaccacatgctatgatgcccactgtgatctccagagcaaagttcgttcgatcgtactgttactctctctctttcaaacagaattgtccgaatcgtgtgacaacaacagcctgttctcacacactcttttcttctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagttttttcaagttcttagatgctttctttttctctttttttacagatcatcaaggaagtaattatctactttttacaacaaatataaaaacatactagagagcttcagcctctcttttatccaaagataccccttcttctttagcagcaatgctggcaatagtagtatttataaacaataacccgttatttgtgctgttggaaaatggcaaaacagcaacatcgaaatccccttctaaatccaagtaaccgatgacagcttcagccggaatttgtgcggtttcatcttctgttgtagtgttgactggagcagctaatgcggaggatgctgcgaataaaacggcagtaaaaattgaaggaaatctcattactagatgcaaacaaatccatatgcaagaggtccaaatccaactgcggcttcattagaggcttctgcgggtccttttactgttagatcttttactgtttcaagaccatctggttatggtgcaggtacagtttattacccaactaatgctggtggtacagttggtgctattgcaattgttccaggttacacagcaagacaatcttcaattaaatggtggggtccaagattggcttcacatggtttcgttgttattactatcgatacaaactctactttggatcaaccatcttcaagatcttcacaacaaatggcagctttgagacaagttgcatctttaaatggtacttcttcatctccaatctatggtaaagttgatacagctagaatgggtgttatgggttggtcaatgggtggtggtggttcattgatctctgcggctaacaacccatctttgaaggcagctgcaccacaagctccatgggattcatctactaacttctcatctgttacagttccaactttgatcttcgcttgtgaaaacgattctatcgctccagttaactcatctgcattgccaatctatgattcaatgtctaggaacgctaagcaattcttggaaattaatggtggttcacattcttgtgctaactctggtaactctaaccaagcattgatcggtaaaaagggtgttgcttggatgaagagattcatggataacgatacaagatactcaactttcgcatgtgaaaacccaaactcaactagagtttctgatttcagaacagctaattgttcttaatactagaggcaaattaaagccttcgagcgtcccaaaaccttctcaagcaaggttttcagtataatgttacatgcgtacacgcgtctgtacagaaaaaaaagaaaaatttgaaatataaataacgttcttaatactaacataactataaaaaaataaatagggacctagacttcaggttgtctaactccttccttttcggttagagcggatgtggggggagggcgtgaatgtaagcgtgacataactaattacatga BBa_K2110017_sequence 1 agcttcagcctctcttttatccaaagataccccttcttctttagcagcaatgctggcaatagtagtatttataaacaataacccgttatttgtgctgttggaaaatggcaaaacagcaacatcgaaatccccttctaaatccaagtaaccgatgacagcttcagccggaatttgtgcggtttcatcttctgttgtagtgttgactggagcagctaatgcggaggatgctgcgaataaaacggcagtaaaaattgaaggaaatctcat BBa_K2110000_sequence 1 gcaaattaaagccttcgagcgtcccaaaaccttctcaagcaaggttttcagtataatgttacatgcgtacacgcgtctgtacagaaaaaaaagaaaaatttgaaatataaataacgttcttaatactaacataactataaaaaaataaatagggacctagacttcaggttgtctaactccttccttttcggttagagcggatgtggggggagggcgtgaatgtaagcgtgacataactaattacatga BBa_K2110800_sequence 1 atgcaaacaaatccatatgcaagaggtccaaatccaactgcggcttcattagaggcttctgcgggtccttttactgttagatcttttactgtttcaagaccatctggttatggtgcaggtacagtttattacccaactaatgctggtggtacagttggtgctattgcaattgttccaggttacacagcaagacaatcttcaattaaatggtggggtccaagattggcttcacatggtttcgttgttattactatcgatacaaactctactttggatcaaccatcttcaagatcttcacaacaaatggcagctttgagacaagttgcatctttaaatggtacttcttcatctccaatctatggtaaagttgatacagctagaatgggtgttatgggttggtcaatgggtggtggtggttcattgatctctgcggctaacaacccatctttgaaggcagctgcaccacaagctccatgggattcatctactaacttctcatctgttacagttccaactttgatcttcgcttgtgaaaacgattctatcgctccagttaactcatctgcattgccaatctatgattcaatgtctaggaacgctaagcaattcttggaaattaatggtggttcacattcttgtgctaactctggtaactctaaccaagcattgatcggtaaaaagggtgttgcttggatgaagagattcatggataacgatacaagatactcaactttcgcatgtgaaaacccaaactcaactagagtttctgatttcagaacagctaattgttcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z