BBa_K2110800 1 BBa_K2110800 PETase 2016-10-04T11:00:00Z 2016-10-06T12:55:00Z Come from the genome of Ideonella sakaiensis (strain 201-F6) The sequence codes the wild type protein called PETase. PETase is a Poly(ethylene terephthalate) hydrolase, which was found in Ideonella sakaiensis (strain 201-F6) by Japanese scientists. It allows Ideonella sakaiensis to degrade PET. If properly transferred into engineered organisms as E.Coli or yeast, secreted PETase can catalyze the hydrolysis of PET to produce mono(2-hydroxyethyl) terephthalate (MHET) as the major product. Optimum temperature is 40 degrees Celsius for PET film hydrolysis and optimum pH is 9. false false _2578_ 32964 32965 9 false This enzyme should be work in extracellular environments. false Haipeng Yu annotation2486182 1 misc range2486182 1 1 795 BBa_K2110800_sequence 1 atgcaaacaaatccatatgcaagaggtccaaatccaactgcggcttcattagaggcttctgcgggtccttttactgttagatcttttactgtttcaagaccatctggttatggtgcaggtacagtttattacccaactaatgctggtggtacagttggtgctattgcaattgttccaggttacacagcaagacaatcttcaattaaatggtggggtccaagattggcttcacatggtttcgttgttattactatcgatacaaactctactttggatcaaccatcttcaagatcttcacaacaaatggcagctttgagacaagttgcatctttaaatggtacttcttcatctccaatctatggtaaagttgatacagctagaatgggtgttatgggttggtcaatgggtggtggtggttcattgatctctgcggctaacaacccatctttgaaggcagctgcaccacaagctccatgggattcatctactaacttctcatctgttacagttccaactttgatcttcgcttgtgaaaacgattctatcgctccagttaactcatctgcattgccaatctatgattcaatgtctaggaacgctaagcaattcttggaaattaatggtggttcacattcttgtgctaactctggtaactctaaccaagcattgatcggtaaaaagggtgttgcttggatgaagagattcatggataacgatacaagatactcaactttcgcatgtgaaaacccaaactcaactagagtttctgatttcagaacagctaattgttcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z