BBa_K2110808
1
BBa_K2110808
PETase site mutation R90A - F
2016-10-04T11:00:00Z
2016-10-11T07:36:40Z
Artificially Designed
This is the forward for mutant PETase. Site R90 was designed to be replaced by A90. The original codon optimized sequence codes the wild type protein called PETase. PETase is a Poly(ethylene terephthalate) hydrolase, which was found in Ideonella sakaiensis (strain 201-F6) by Japanese scientists. It allows Ideonella sakaiensis to degrade PET. If properly transferred into engineered organisms as E.Coli or yeast, secreted PETase can catalyze the hydrolysis of PET to produce mono(2-hydroxyethyl) terephthalate (MHET) as the major product. Optimum temperature is 40 degrees Celsius for PET film hydrolysis and optimum pH is 9.
true
false
_2578_
32964
32965
9
false
First apply PCR for mutant section. Then PCR again with original primers of PETase to overlap for the entire mutant PETase.
false
Haipeng Yu
BBa_K2110808_sequence
1
aggttacacagcagcacaatcttcaattaaat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z