BBa_K2111000
1
BBa_K2111000
RI7_FLAG_OXTR
2016-10-18T11:00:00Z
2016-10-19T05:11:50Z
The human oxytocin receptor sequence was found in the NCBI database, codon optimized for saccharomyce cerevisiae using http://www.jcat.de/ (with additional options to avoid cleavage sites EcoR1, Pst1, Spe1, Xba1. The sequence for the rat RI7 OR was found on the 2012 TU Delft iGEM team page and in Rhadhika 2007 (http://www.nature.com/nchembio/journal/v3/n6/full/nchembio882.html).
This part is a chimeric receptor with a FLAG tag. It is composed of the ligand biding domain of the human oxytocin receptor, flanked by the N and C termini of the rat RI7 olfactory receptor. It was designed using the DIY receptor design guide created by the 2012 TU Delft iGEM team (http://2012.igem.org/Team:TU-Delft/receptordesign). This part is intended to express in the membrane of a yeast cell and - upon oxytocin ligand binding - activate the mating pathway and the inducible FUS1 promotor. This allows us to leverage a reporter protein under control of FUS1 to detect the presence of oxytocin.
false
false
_2579_
31485
31485
9
false
We added a FLAG tag to facilitate SDS-PAGE. We added the yeast kozak sequence tacaaa to improve translation and codon optimized for saccharomyce cerevisiae.
false
Isaac Bean
annotation2523800
1
RI7 N-terminus
range2523800
1
34
213
annotation2524246
1
RI7 C-terminus
range2524246
1
1018
1116
annotation2524242
1
Human Oxytocin Receptor Binding Site
range2524242
1
214
1017
annotation2524445
1
Stop
range2524445
1
1117
1122
annotation2524194
1
Start
range2524194
1
7
9
annotation2523657
1
FLAG tag
range2523657
1
10
33
annotation2522995
1
Kozak
range2522995
1
1
6
BBa_K2111000_sequence
1
tacaaaatggactataaagatgacgatgataaggaacgtcgtaaccattccggtagagtgtctgagttcgtgttgttaggttttcccgcgccggccccccttcgtgtgttgctttttttcctgtctctactggcatatgtacttgtattaacggaaaacatgttgattattatagccatcagaaaccatcctactttacataagccgatgtataggacgacaagacaaaaacacagtcgtctatttttctttatgaagcatttaagtatagccgacttagtcgtcgctgtgtttcaggttctgccccagttactgtgggatattacattccgtttttacggccctgacttgctgtgtagattagtgaaatatctacaggtcgtaggtatgtttgcttcaacctatcttctattactaatgagtcttgatcgttgcttagctatatgccagccattgcgttcccttcgtaggaggacggatcgtcttgctgtccttgcgacgtggctaggctgtcttgtcgccagtgcaccgcaagtacatatcttcagtttacgtgaagtcgcggacggtgtctttgactgttgggcggtattcatccagccttggggtccaaaggcatacattacgtggattactctggccgtctatattgtgcctgtaatagttttggcagcatgttatggtctaatcagctttaagatctggcagaacctacgtctaaagactgctgccgctgcggctgctgaggctccggagggagcagctgccggggacggcggaagggtagccttggcacgtgtcagcagtgtaaaactaattagcaaagcaaaaataagaactgtgaagatgacattcattatcgttcttgccttcatcgtgtgttggacgcccttcttttttgtgcaaatgtggagtgtctgggatgcaaacgcaccaaaggaagcctcagcctttattattgtgatgttattggcgtcactaaacagttgttgcaatccctggatatacatgctgttctattgcttacgtaaccaagacgtcaaacgtgcgttaagaaggacgttacatctggcacaagatcaggaggcgaatactaataagggctctaagatcggctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z