LOCUS       BBa_K2111001            2002 bp    DNA     linear       25-Jun-2024
DEFINITION  PGK1_OXTR
ACCESSION   BBa_K2111001
VERSION     BBa_K2111001.1  
FEATURES             Location/Qualifiers
     promoter        1..872
                     /label=pPGK1
     misc_feature    740..756
                     /label=Gal4 site
     misc_feature    758..774
                     /label=Gal4 site
     misc_feature    724..729
                     /label=TATA?
     misc_feature    864..870
                     /label=TATA?
     promoter        1..872
                     /label=BBa_K1652000
     RBS             881..886
                     /label=Kozak
     misc_feature    890..913
                     /label=FLAG tag
     misc_feature    914..1093
                     /label=RI7 N-terminus
     misc_feature    887..889
                     /label=Start
     misc_feature    1094..1897
                     /label=Human Oxytocin Receptor Binding Site
     misc_feature    1898..1996
                     /label=RI7 C-terminus
     misc_feature    1997..2002
                     /label=Stop
     CDS             881..2002
                     /label=BBa_K2111000
ORIGIN
        1 actgtaattg cttttagttg tgtattttta gtgtgcaagt ttctgtaaat cgattaattt
       61 ttttttcttt cctcttttta ttaaccttaa tttttatttt agattcctga cttcaactca
      121 agacgcacag atattataac atctgcataa taggcatttg caagaattac tcgtgagtaa
      181 ggaaagagtg aggaactatc gcatacctgc atttaaagat gccgatttgg gcgcgaatcc
      241 tttattttgg cttcaccctc atactattat cagggccaga aaaaggaagt gtttccctcc
      301 ttcttgaatt gatgttaccc tcataaagca cgtggcctct tatcgagaaa gaaattaccg
      361 tcgctcgtga tttgtttgca aaaagaacaa aactgaaaaa acccagacac gctcgacttc
      421 ctgtcttcct attgattgca gcttccaatt tcgtcacaca acaaggtcct agcgacggct
      481 cacaggtttt gtaacaagca atcgaaggtt ctggaatggc gggaaagggt ttagtaccac
      541 atgctatgat gcccactgtg atctccagag caaagttcgt tcgatcgtac tgttactctc
      601 tctctttcaa acagaattgt ccgaatcgtg tgacaacaac agcctgttct cacacactct
      661 tttcttctaa ccaagggggt ggtttagttt agtagaacct cgtgaaactt acatttacat
      721 atatataaac ttgcataaac gggcgacagc cctccgtcgg gcgacagccc tccgtaattc
      781 gtagtttttc aagttcttag atgctttctt tttctctttt ttacagatca tcaaggaagt
      841 aattatctac tttttacaac aaatataaaa catactagag tacaaaatgg actataaaga
      901 tgacgatgat aaggaacgtc gtaaccattc cggtagagtg tctgagttcg tgttgttagg
      961 ttttcccgcg ccggcccccc ttcgtgtgtt gctttttttc ctgtctctac tggcatatgt
     1021 acttgtatta acggaaaaca tgttgattat tatagccatc agaaaccatc ctactttaca
     1081 taagccgatg tataggacga caagacaaaa acacagtcgt ctatttttct ttatgaagca
     1141 tttaagtata gccgacttag tcgtcgctgt gtttcaggtt ctgccccagt tactgtggga
     1201 tattacattc cgtttttacg gccctgactt gctgtgtaga ttagtgaaat atctacaggt
     1261 cgtaggtatg tttgcttcaa cctatcttct attactaatg agtcttgatc gttgcttagc
     1321 tatatgccag ccattgcgtt cccttcgtag gaggacggat cgtcttgctg tccttgcgac
     1381 gtggctaggc tgtcttgtcg ccagtgcacc gcaagtacat atcttcagtt tacgtgaagt
     1441 cgcggacggt gtctttgact gttgggcggt attcatccag ccttggggtc caaaggcata
     1501 cattacgtgg attactctgg ccgtctatat tgtgcctgta atagttttgg cagcatgtta
     1561 tggtctaatc agctttaaga tctggcagaa cctacgtcta aagactgctg ccgctgcggc
     1621 tgctgaggct ccggagggag cagctgccgg ggacggcgga agggtagcct tggcacgtgt
     1681 cagcagtgta aaactaatta gcaaagcaaa aataagaact gtgaagatga cattcattat
     1741 cgttcttgcc ttcatcgtgt gttggacgcc cttctttttt gtgcaaatgt ggagtgtctg
     1801 ggatgcaaac gcaccaaagg aagcctcagc ctttattatt gtgatgttat tggcgtcact
     1861 aaacagttgt tgcaatccct ggatatacat gctgttctat tgcttacgta accaagacgt
     1921 caaacgtgcg ttaagaagga cgttacatct ggcacaagat caggaggcga atactaataa
     1981 gggctctaag atcggctaat aa
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