BBa_K2111005
1
BBa_K2111005
FUS1_iLOV
2016-10-18T11:00:00Z
2016-10-19T06:52:40Z
We used our FUS1 part (BBa_K2111002) and iLOV (BBa_K660004) from the 2011 Glasgow iGEM team.
This is a composite of the FUS1 promoter (BBa_K2111002) and iLOV (BBa_K660004). It is a reporter part used to visualize activation of the yeast mating pathway.
false
false
_2579_
31485
31485
9
false
We used these parts to obtain a visible visible reporter.
false
Isaac Bean
component2523777
1
BBa_K2111002
component2523779
1
BBa_K660004
annotation2523779
1
BBa_K660004
range2523779
1
208
543
annotation2523777
1
BBa_K2111002
range2523777
1
1
201
BBa_K2111002
1
BBa_K2111002
FUS1 Promoter
2016-10-18T11:00:00Z
2016-10-19T06:59:22Z
We used the sequence from BBa_K775004, with confirmation from the NCBI database for use in yeast strain S288c.
The FUS1 promoter is from the yeast mating pathway. It is induced with pheromones from the opposite mating type.
false
false
_2579_
31485
31485
9
Not in stock
false
We confirmed that the sequence was found in the S288c strain.
false
Isaac Bean
annotation2523764
1
FUS1
range2523764
1
1
201
BBa_K660004
1
BBa_K660004
iLOV
2011-09-15T11:00:00Z
2015-05-08T01:13:03Z
iLOV is a version of LOV2 that has been altered through site directed mutagenesis and DNA shuffling.
Released HQ 2013
iLOV has the same function and uses as LOV2. As a reporter, it is advantageous over GFP derived fluorescent proteins due to its small size (useful if you are tagging proteins), ability to recover quickly from photobleaching and use in anoxic conditions.
false
false
_837_
0
9421
9
In stock
true
illegal restriction sites. The part was synthesized to remove them.
false
Glasgow Uni 2011
annotation2135615
1
iLOV Domain
range2135615
1
1
336
BBa_K2111005_sequence
1
tttggtgcgatgatgaaacaaacatgaaacgtctgtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatcttttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaatactagatgattgaaaaaaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatggctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaacgcgcgttttttgcagggcccggaaaccgatcaggcgaccgtgcagaaaattcgtgatgcgattcgtgatcagcgtgaaaccaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgctgcatttgcagccggtgcgtgatcagaaaggcgaattgcagtattttattggcgtgcagctggatggcagcgatcatgtgtaa
BBa_K660004_sequence
1
atgattgaaaaaaactttgtgattaccgatccgcgtctgccggataacccgattatttttgcgagcgatggctttctggaactgaccgaatatagccgtgaagaaattctgggccgtaacgcgcgttttttgcagggcccggaaaccgatcaggcgaccgtgcagaaaattcgtgatgcgattcgtgatcagcgtgaaaccaccgtgcagctgattaactataccaaaagcggcaaaaaattttggaacctgctgcatttgcagccggtgcgtgatcagaaaggcgaattgcagtattttattggcgtgcagctggatggcagcgatcatgtgtaa
BBa_K2111002_sequence
1
tttggtgcgatgatgaaacaaacatgaaacgtctgtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatcttttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z