BBa_K215091 1 BBa_K215091 pLac+RBS+OpdA 2009-09-30T11:00:00Z 2015-05-08T01:11:30Z see K215090 for OpdA source. Strong IPTG-inducible expression of the OpdA enzyme. false false _320_ 0 2811 9 It's complicated true n/a false Chris Eiben component2219497 1 BBa_K215090 component2219496 1 BBa_K215000 annotation2219496 1 BBa_K215000 range2219496 1 1 75 annotation2219497 1 BBa_K215090 range2219497 1 82 1101 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K215090 1 BBa_K215090 OpdA (phosphotriesterase) 2009-09-29T11:00:00Z 2015-05-08T01:11:30Z Agrobacterium Opda is a phophotriesterase from Agrobacterium that can detoxify a broad range of organophosphate pesticides and nerve agents. false false _320_ 0 2811 9 It's complicated true All biobrick restriction sites have been removed. false Chris Eiben BBa_K2112001 1 BBa_K2112001 OP Pesticides Residue Killer 2016-10-13T11:00:00Z 2016-10-14T06:03:07Z The ccdB gene is found by other teams, we use it in our part to achieve the goal. The plasmid could be co-transformed into E.coli BL21(DE3) with another plasmid containing a lacI coding sequence. Then with the inducing by IPTG (a compound), target protein could be expressed in high efficiency. T7 promoter is a strong promoter to launch the transcription procedure. Lac operator DNA sequence could bind a protein named LacI coded by a lacI DNA sequence which located in another plasmid. This process stop the transcription of the target gene (opdA). So in our study, we add a compound named IPTG to the culture medium. IPTG could bind LacI protein, so that the structure of LacI will be changed and could not bind to lac operator DNA sequence any more. And the target gene opdA could be transcribed and translated. His-tag sequence (fused in N-terminal of target protein OpdA) consisted of five to six histidines, which could bind to Nickel metal ions. So with a special resin chelated with nickel metal ions, we can purify the expressed OpdA protein easily. When??the??degradation??finishes,??ultraviolet??light??or??natural??light??is??given??to??shed??on??the??bacteria,??and??thus??the??RecA??repair??gene??inside??the??bacteria??is??activated.??The??product??of??this??gene??can??activate??RecA??(SOS)??promoter.??Thus??the??bacteria??begin??to??express??suicide??gene??ccdB,??which??leads??the??bacteria??to??dying??automatically??in??order??to??avoid??the??secondary??pollution??on??vegetables??and??fruits??posed??by??genetically??engineered??bacteria. false false _2580_ 27358 27358 9 false The his-tag can allow us to purify the protein after the bacteria secrete it, so that if the project can be use in real life, the posibility of risk can be limited by giving the users the purified protein to degrade organophosphorous instead of using the bacteria. false Fanglin Zhao component2502651 1 BBa_K145151 component2502635 1 BBa_K215090 component2502645 1 BBa_K215091 component2502646 1 BBa_K1010008 component2502647 1 BBa_J22106 component2502655 1 BBa_K1010007 annotation2502651 1 BBa_K145151 range2502651 1 3415 3720 annotation2502645 1 BBa_K215091 range2502645 1 1021 2121 annotation2502655 1 BBa_K1010007 range2502655 1 3721 4026 annotation2502647 1 BBa_J22106 range2502647 1 3223 3414 annotation2502635 1 BBa_K215090 range2502635 1 1 1020 annotation2502646 1 BBa_K1010008 range2502646 1 2122 3222 BBa_K215000 1 BBa_K215000 R0011+B0034, strong IPTG-inducible promoter with strong RBS. 2009-09-29T11:00:00Z 2015-05-08T01:11:29Z existing registry parts This part contains the strong, IPTG-inducible promoter R0011, combined with the strong RBS B0034. This part can be used as a strong protein expression system when combined with a protein coding sequence. false false _320_ 0 2811 9 It's complicated true This part should offer greater levels of expression when compared to similar parts using the natural lac promoter (R0010). false Chris Eiben component2027435 1 BBa_R0011 component2027441 1 BBa_B0034 annotation2027441 1 BBa_B0034 range2027441 1 64 75 annotation2027435 1 BBa_R0011 range2027435 1 1 54 BBa_K1010007 1 BBa_K1010007 sucide protein ccdB 2013-09-17T11:00:00Z 2015-07-09T11:31:24Z From existing parts in Registry. BBa_R0010 + BBa_BOO34 +BBa_K145151 + BBa_C0012 Part of a suicide system. true false _1316_ 4206 16283 9 In stock false This part contains the promoter, rbs and terminator. false Junyu Luo annotation2370084 1 stop range2370084 1 304 306 annotation2370082 1 start range2370082 1 1 3 annotation2370083 1 cds range2370083 1 1 303 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2001 1 lac O1 range2001 1 26 42 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation1999 1 lac O1 range1999 1 3 19 annotation2000 1 -35 range2000 1 20 25 annotation2002 1 -10 range2002 1 43 48 BBa_J22106 1 BBa_J22106 rec A (SOS) Promoter 2006-09-04T11:00:00Z 2015-08-31T04:08:38Z The recA(SOS)promoter gene from the chromosomal DNA isolated from MG1655 has been PCR amplified and inserted into plasmid pSB1A2(part number BBa_B0015). Rec A (SOS) promoter. false true _70_ 0 1038 70 In stock false NA true Krithiga Sankaran BBa_K145151 1 ccdB ccdB coding region 2008-08-06T11:00:00Z 2015-07-08T03:14:50Z P1010 Released HQ 2013 Coding region for the ccdB (control of cell death) gene. true false _257_ 4206 2970 9 In stock true just one stop codon in the end true Jonas Demeulemeester annotation1970252 1 stop range1970252 1 304 306 annotation1970253 1 cds range1970253 1 1 303 annotation1970251 1 start range1970251 1 1 3 BBa_K1010008 1 BBa_K1010008 Organophosphorus Hydrolase, OPH 2013-09-17T11:00:00Z 2015-05-08T01:08:41Z From Flavobacterium sp An organophosphorus hydrolase(OPH) than can degrade organophosphorus compounds false false _1316_ 0 16283 9 It's complicated false Serval sites are mutated. false Junyu Luo BBa_K2112001_sequence 1 atgggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagcaccaccaccaccaccactgaaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagcaccaccaccaccaccactgaaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagcaccaccaccaccaccactgaaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttcaacagaacatattgactatccggtattacccggcatgacaggagtaaaaatggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctccatcatgtaataaatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataaatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa BBa_K1010008_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagcaccaccaccaccaccactga BBa_J22106_sequence 1 aacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttcaacagaacatattgactatccggtattacccggcatgacaggagtaaaaatggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctccatcatgtaataa BBa_K145151_sequence 1 atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K215091_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagcaccaccaccaccaccactga BBa_K215000_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa BBa_K215090_sequence 1 atgggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagcaccaccaccaccaccactga BBa_K1010007_sequence 1 atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z