BBa_K2112001
type: ComponentDefinition
persistent identity: BBa K2112001
display id: BBa_K2112001
version: 1
was generated by: iGEM to SBOL conversion
description: OP Pesticides Residue Killer
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T06:03:07Z
mutable provenance: The ccdB gene is found by other teams, we use it in our part to achieve the goal.
owned by: james
owned by: myers
mutable description: The plasmid could be co-transformed into E.coli BL21(DE3) with another plasmid containing a lacI coding sequence. Then with the inducing by IPTG (a compound), target protein could be expressed in high efficiency. T7 promoter is a strong promoter to launch the transcription procedure. Lac operator DNA sequence could bind a protein named LacI coded by a lacI DNA sequence which located in another plasmid. This process stop the transcription of the target gene (opdA). So in our study, we add a compound named IPTG to the culture medium. IPTG could bind LacI protein, so that the structure of LacI will be changed and could not bind to lac operator DNA sequence any more. And the target gene opdA could be transcribed and translated. His-tag sequence (fused in N-terminal of target protein OpdA) consisted of five to six histidines, which could bind to Nickel metal ions. So with a special resin chelated with nickel metal ions, we can purify the expressed OpdA protein easily. When??the??degradation??finishes,??ultraviolet??light??or??natural??light??is??given??to??shed??on??the??bacteria,??and??thus??the??RecA??repair??gene??inside??the??bacteria??is??activated.??The??product??of??this??gene??can??activate??RecA??(SOS)??promoter.??Thus??the??bacteria??begin??to??express??suicide??gene??ccdB,??which??leads??the??bacteria??to??dying??automatically??in??order??to??avoid??the??secondary??pollution??on??vegetables??and??fruits??posed??by??genetically??engineered??bacteria.
discontinued: false
dominant: false
group u list: _2580_
m user id: 27358
owner id: 27358
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The his-tag can allow us to purify the protein after the bacteria secrete it, so that if the project can be use in real life, the posibility of risk can be limited by giving the users the purified protein to degrade organophosphorous instead of using the bacteria.
star: false
creator: Fanglin Zhao
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1010007
component: BBa_K145151
component: BBa_J22106
component: BBa_K1010008
component: BBa_K215091
component: BBa_K215090
sequence annotation: BBa_K215090
sequence annotation: BBa_K215091
sequence annotation: BBa_K1010008
sequence annotation: BBa_J22106
sequence annotation: BBa_K145151
sequence annotation: BBa_K1010007
sequence: https://synbiohub.org/public/igem/BBa_K2112001_sequence/1

BBa_K1010007
type: Component
persistent identity: component2502655
display id: component2502655
version: 1
top level: BBa_K2112001
definition: BBa_K1010007
access: public

BBa_K145151
type: Component
persistent identity: component2502651
display id: component2502651
version: 1
top level: BBa_K2112001
definition: ccdB
access: public

BBa_J22106
type: Component
persistent identity: component2502647
display id: component2502647
version: 1
top level: BBa_K2112001
definition: BBa_J22106
access: public

BBa_K1010008
type: Component
persistent identity: component2502646
display id: component2502646
version: 1
top level: BBa_K2112001
definition: BBa_K1010008
access: public

BBa_K215091
type: Component
persistent identity: component2502645
display id: component2502645
version: 1
top level: BBa_K2112001
definition: BBa_K215091
access: public

BBa_K215090
type: Component
persistent identity: component2502635
display id: component2502635
version: 1
top level: BBa_K2112001
definition: BBa_K215090
access: public

BBa_K215090
type: SequenceAnnotation
persistent identity: annotation2502635
display id: annotation2502635
version: 1
top level: BBa_K2112001
location: https://synbiohub.org/public/igem/BBa_K2112001/annotation2502635/range2502635/1
component: BBa_K215090

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type: Range
persistent identity: range2502635
display id: range2502635
version: 1
top level: BBa_K2112001
direction: direction/reverse
start: 1
end: 1020
orientation: inline

BBa_K215091
type: SequenceAnnotation
persistent identity: annotation2502645
display id: annotation2502645
version: 1
top level: BBa_K2112001
location: https://synbiohub.org/public/igem/BBa_K2112001/annotation2502645/range2502645/1
component: BBa_K215091

https://synbiohub.org/public/igem/BBa_K2112001/annotation2502645/range2502645/1
type: Range
persistent identity: range2502645
display id: range2502645
version: 1
top level: BBa_K2112001
direction: direction/reverse
start: 1021
end: 2121
orientation: inline

BBa_K1010008
type: SequenceAnnotation
persistent identity: annotation2502646
display id: annotation2502646
version: 1
top level: BBa_K2112001
location: https://synbiohub.org/public/igem/BBa_K2112001/annotation2502646/range2502646/1
component: BBa_K1010008

https://synbiohub.org/public/igem/BBa_K2112001/annotation2502646/range2502646/1
type: Range
persistent identity: range2502646
display id: range2502646
version: 1
top level: BBa_K2112001
direction: direction/reverse
start: 2122
end: 3222
orientation: inline

BBa_J22106
type: SequenceAnnotation
persistent identity: annotation2502647
display id: annotation2502647
version: 1
top level: BBa_K2112001
location: https://synbiohub.org/public/igem/BBa_K2112001/annotation2502647/range2502647/1
component: BBa_J22106

https://synbiohub.org/public/igem/BBa_K2112001/annotation2502647/range2502647/1
type: Range
persistent identity: range2502647
display id: range2502647
version: 1
top level: BBa_K2112001
direction: direction/reverse
start: 3223
end: 3414
orientation: inline

BBa_K145151
type: SequenceAnnotation
persistent identity: annotation2502651
display id: annotation2502651
version: 1
top level: BBa_K2112001
location: https://synbiohub.org/public/igem/BBa_K2112001/annotation2502651/range2502651/1
component: BBa_K145151

https://synbiohub.org/public/igem/BBa_K2112001/annotation2502651/range2502651/1
type: Range
persistent identity: range2502651
display id: range2502651
version: 1
top level: BBa_K2112001
direction: direction/reverse
start: 3415
end: 3720
orientation: inline

BBa_K1010007
type: SequenceAnnotation
persistent identity: annotation2502655
display id: annotation2502655
version: 1
top level: BBa_K2112001
location: https://synbiohub.org/public/igem/BBa_K2112001/annotation2502655/range2502655/1
component: BBa_K1010007

https://synbiohub.org/public/igem/BBa_K2112001/annotation2502655/range2502655/1
type: Range
persistent identity: range2502655
display id: range2502655
version: 1
top level: BBa_K2112001
direction: direction/reverse
start: 3721
end: 4026
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa K145151
display id: BBa_K145151
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB coding region
created: 2008-08-06T11:00:00Z
modified: 2015-07-08T03:14:50Z
mutable provenance: P1010
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the ccdB (control of cell death) gene.
discontinued: true
dominant: false
experience: experience/None
group u list: _257_
m user id: 4206
owner id: 2970
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: true
mutable notes: just one stop codon in the end
star: true
creator: Jonas Demeulemeester
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: cds
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K145151_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1970251
display id: annotation1970251
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K145151/annotation1970251/range1970251/1
type: Range
persistent identity: range1970251
display id: range1970251
version: 1
top level: ccdB
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation1970253
display id: annotation1970253
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K145151/annotation1970253/range1970253/1
type: Range
persistent identity: range1970253
display id: range1970253
version: 1
top level: ccdB
direction: direction/forward
start: 1
end: 303
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1970252
display id: annotation1970252
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K145151/annotation1970252/range1970252/1
type: Range
persistent identity: range1970252
display id: range1970252
version: 1
top level: ccdB
direction: direction/forward
start: 304
end: 306
orientation: inline

BBa_J22106
type: ComponentDefinition
persistent identity: BBa J22106
display id: BBa_J22106
version: 1
was generated by: iGEM to SBOL conversion
description: rec A (SOS) Promoter
created: 2006-09-04T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: The recA(SOS)promoter gene from the chromosomal DNA isolated from MG1655 has been PCR amplified and inserted into plasmid pSB1A2(part number BBa_B0015).
owned by: james
owned by: myers
mutable description: Rec A (SOS) promoter.
discontinued: false
dominant: true
group u list: _70_
m user id: 0
owner id: 1038
owning group id: 70
sample status: In stock
status: status/Available
bookmark: false
mutable notes: NA
star: true
creator: Krithiga Sankaran
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J22106_sequence/1

BBa_K215090
type: ComponentDefinition
persistent identity: BBa K215090
display id: BBa_K215090
version: 1
was generated by: iGEM to SBOL conversion
description: OpdA (phosphotriesterase)
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: Agrobacterium
owned by: james
owned by: myers
mutable description: Opda is a phophotriesterase from Agrobacterium that can detoxify a broad range of organophosphate pesticides and nerve agents.
discontinued: false
dominant: false
experience: experience/Works
group u list: _320_
m user id: 0
owner id: 2811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: All biobrick restriction sites have been removed.
star: false
creator: Chris Eiben
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K215090_sequence/1

BBa_K215091
type: ComponentDefinition
persistent identity: BBa K215091
display id: BBa_K215091
version: 1
was generated by: iGEM to SBOL conversion
description: pLac+RBS+OpdA
created: 2009-09-30T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: see K215090 for OpdA source.
owned by: james
owned by: myers
mutable description: Strong IPTG-inducible expression of the OpdA enzyme.
discontinued: false
dominant: false
experience: experience/Works
group u list: _320_
m user id: 0
owner id: 2811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: n/a
star: false
creator: Chris Eiben
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K215090
component: BBa_K215000
sequence annotation: BBa_K215000
sequence annotation: BBa_K215090
sequence: https://synbiohub.org/public/igem/BBa_K215091_sequence/1

BBa_K215090
type: Component
persistent identity: component2219497
display id: component2219497
version: 1
top level: BBa_K215091
definition: BBa_K215090
access: public

BBa_K215000
type: Component
persistent identity: component2219496
display id: component2219496
version: 1
top level: BBa_K215091
definition: BBa_K215000
access: public

BBa_K215000
type: SequenceAnnotation
persistent identity: annotation2219496
display id: annotation2219496
version: 1
top level: BBa_K215091
location: https://synbiohub.org/public/igem/BBa_K215091/annotation2219496/range2219496/1
component: BBa_K215000

https://synbiohub.org/public/igem/BBa_K215091/annotation2219496/range2219496/1
type: Range
persistent identity: range2219496
display id: range2219496
version: 1
top level: BBa_K215091
direction: direction/reverse
start: 1
end: 75
orientation: inline

BBa_K215090
type: SequenceAnnotation
persistent identity: annotation2219497
display id: annotation2219497
version: 1
top level: BBa_K215091
location: https://synbiohub.org/public/igem/BBa_K215091/annotation2219497/range2219497/1
component: BBa_K215090

https://synbiohub.org/public/igem/BBa_K215091/annotation2219497/range2219497/1
type: Range
persistent identity: range2219497
display id: range2219497
version: 1
top level: BBa_K215091
direction: direction/reverse
start: 82
end: 1101
orientation: inline

BBa_K1010007
type: ComponentDefinition
persistent identity: BBa K1010007
display id: BBa_K1010007
version: 1
was generated by: iGEM to SBOL conversion
description: sucide protein ccdB
created: 2013-09-17T11:00:00Z
modified: 2015-07-09T11:31:24Z
mutable provenance: From existing parts in Registry.
owned by: james
owned by: myers
mutable description: BBa_R0010 + BBa_BOO34 +BBa_K145151 + BBa_C0012 Part of a suicide system.
discontinued: true
dominant: false
experience: experience/None
group u list: _1316_
m user id: 4206
owner id: 16283
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: false
mutable notes: This part contains the promoter, rbs and terminator.
star: false
creator: Junyu Luo
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: cds
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1010007_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2370083
display id: annotation2370083
version: 1
top level: BBa_K1010007
location: https://synbiohub.org/public/igem/BBa_K1010007/annotation2370083/range2370083/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1010007/annotation2370083/range2370083/1
type: Range
persistent identity: range2370083
display id: range2370083
version: 1
top level: BBa_K1010007
direction: direction/forward
start: 1
end: 303
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2370082
display id: annotation2370082
version: 1
top level: BBa_K1010007
location: https://synbiohub.org/public/igem/BBa_K1010007/annotation2370082/range2370082/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K1010007/annotation2370082/range2370082/1
type: Range
persistent identity: range2370082
display id: range2370082
version: 1
top level: BBa_K1010007
direction: direction/forward
start: 1
end: 3
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2370084
display id: annotation2370084
version: 1
top level: BBa_K1010007
location: https://synbiohub.org/public/igem/BBa_K1010007/annotation2370084/range2370084/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1010007/annotation2370084/range2370084/1
type: Range
persistent identity: range2370084
display id: range2370084
version: 1
top level: BBa_K1010007
direction: direction/forward
start: 304
end: 306
orientation: inline

BBa_K1010008
type: ComponentDefinition
persistent identity: BBa K1010008
display id: BBa_K1010008
version: 1
was generated by: iGEM to SBOL conversion
description: Organophosphorus Hydrolase, OPH
created: 2013-09-17T11:00:00Z
modified: 2015-05-08T01:08:41Z
mutable provenance: From Flavobacterium sp
owned by: james
owned by: myers
mutable description: An organophosphorus hydrolase(OPH) than can degrade organophosphorus compounds
discontinued: false
dominant: false
group u list: _1316_
m user id: 0
owner id: 16283
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Serval sites are mutated.
star: false
creator: Junyu Luo
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1010008_sequence/1

BBa_K215000
type: ComponentDefinition
persistent identity: BBa K215000
display id: BBa_K215000
version: 1
was generated by: iGEM to SBOL conversion
description: R0011+B0034, strong IPTG-inducible promoter with strong RBS.
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:29Z
mutable provenance: existing registry parts
owned by: james
owned by: myers
mutable description: This part contains the strong, IPTG-inducible promoter R0011, combined with the strong RBS B0034. This part can be used as a strong protein expression system when combined with a protein coding sequence.
discontinued: false
dominant: false
experience: experience/Works
group u list: _320_
m user id: 0
owner id: 2811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This part should offer greater levels of expression when compared to similar parts using the natural lac promoter (R0010).
star: false
creator: Chris Eiben
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K215000_sequence/1

BBa_B0034
type: Component
persistent identity: component2027441
display id: component2027441
version: 1
top level: BBa_K215000
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2027435
display id: component2027435
version: 1
top level: BBa_K215000
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2027435
display id: annotation2027435
version: 1
top level: BBa_K215000
location: https://synbiohub.org/public/igem/BBa_K215000/annotation2027435/range2027435/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K215000/annotation2027435/range2027435/1
type: Range
persistent identity: range2027435
display id: range2027435
version: 1
top level: BBa_K215000
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2027441
display id: annotation2027441
version: 1
top level: BBa_K215000
location: https://synbiohub.org/public/igem/BBa_K215000/annotation2027441/range2027441/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K215000/annotation2027441/range2027441/1
type: Range
persistent identity: range2027441
display id: range2027441
version: 1
top level: BBa_K215000
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_K2112001_sequence/1
type: Sequence
persistent identity: BBa K2112001 sequence
display id: BBa_K2112001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagcaccaccaccaccaccactgaaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K145151_sequence/1
type: Sequence
persistent identity: BBa K145151 sequence
display id: BBa_K145151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J22106_sequence/1
type: Sequence
persistent identity: BBa J22106 sequence
display id: BBa_J22106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttcaacagaacatattgactatccggtattacccggcatgacaggagtaaaaatggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctccatcatgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K215090_sequence/1
type: Sequence
persistent identity: BBa K215090 sequence
display id: BBa_K215090_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagcaccaccaccaccaccactga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K215091_sequence/1
type: Sequence
persistent identity: BBa K215091 sequence
display id: BBa_K215091_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagcaccaccaccaccaccactga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1010007_sequence/1
type: Sequence
persistent identity: BBa K1010007 sequence
display id: BBa_K1010007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1010008_sequence/1
type: Sequence
persistent identity: BBa K1010008 sequence
display id: BBa_K1010008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagcaccaccaccaccaccactga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K215000_sequence/1
type: Sequence
persistent identity: BBa K215000 sequence
display id: BBa_K215000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z