BBa_K2112015 1 BBa_K2112015 OP Pesticides Residue Degrading 2016-10-13T11:00:00Z 2016-10-31T08:24:35Z From other plasmid. The plasmid could be co-transformed into E.coli BL21(DE3) with another plasmid containing a lacI coding sequence. Then with the inducing by IPTG (a compound), target protein could be expressed in high efficiency. T7 promoter is a strong promoter to launch the transcription procedure. Lac operator DNA sequence could bind a protein named LacI coded by a lacI DNA sequence which located in another plasmid. This process stop the transcription of the target gene (opdA). So in our study, we add a compound named IPTG to the culture medium. IPTG could bind LacI protein, so that the structure of LacI will be changed and could not bind to lac operator DNA sequence any more. And the target gene opdA could be transcribed and translated. His-tag sequence (fused in N-terminal of target protein OpdA) consisted of five to six histidines, which could bind to Nickel metal ions. So with a special resin chelated with nickel metal ions, we can purify the expressed OpdA protein easily. When??the??degradation??finishes,??ultraviolet??light??or??natural??light??is??given??to??shed??on??the??bacteria,??and??thus??the??RecA??repair??gene??inside??the??bacteria??is??activated.??The??product??of??this??gene??can??activate??RecA??(SOS)??promoter.??Thus??the??bacteria??begin??to??express??suicide??gene??ccdB,??which??leads??the??bacteria??to??dying??automatically??in??order??to??avoid??the??secondary??pollution??on??vegetables??and??fruits??posed??by??genetically??engineered??bacteria. false false _2580_ 27358 27358 9 false To avoid second pollution on vegetables posed by genetically engineered bacteria. false Fanglin Zhao component2505759 1 BBa_K2112011 component2505762 1 BBa_K1667004 component2505760 1 BBa_K1667002 component2505761 1 BBa_K1667003 annotation2505760 1 BBa_K1667002 range2505760 1 159 1226 annotation2505759 1 BBa_K2112011 range2505759 1 1 152 annotation2505762 1 BBa_K1667004 range2505762 1 1482 1826 annotation2505761 1 BBa_K1667003 range2505761 1 1235 1473 BBa_K2112011 1 BBa_K2112011 Fragment 1 2016-10-13T11:00:00Z 2016-10-14T08:05:06Z From other plasmid. T7 promoter is a strong promoter to launch the transcription procedure. Lac operator DNA sequence could bind a protein named LacI coded by a lacI DNA sequence which located in another plasmid. This process stop the transcription of the target gene (opdA). So in our study, we add a compound named IPTG to the culture medium. IPTG could bind LacI protein, so that the structure of LacI will be changed and could not bind to lac operator DNA sequence any more. And the target gene opdA could be transcribed and translated. His-tag sequence (fused in N-terminal of target protein OpdA) consisted of five to six histidines, which could bind to Nickel metal ions. So with a special resin chelated with nickel metal ions, we can purify the expressed OpdA protein easily. false false _2580_ 27358 27358 9 false Adding five additional amino acid behind His-tag to ensure a distance exists between His-tag and the target protein so that the protein can work better. false Fanglin Zhao BBa_K1667004 1 BBa_K1667004 suicide gene ccdB 2015-09-06T11:00:00Z 2015-09-07T06:58:03Z The sequence of the ccdB gene in this part is from BB_K145151 and BB_K1010007 in igem. The gene of ccdB is a lethal gene. The expression of ccdB gene will lead to the death of the host cells. false false _2085_ 27665 27665 9 false There is a rbs sequence in front of the ccdB gene fragment to ensure the translation of it. false Jinyan Chu BBa_K1667003 1 BBa_K1667003 RecA promoter 2015-09-06T11:00:00Z 2015-09-07T08:36:43Z There is a DNA spacer fragment in front of the promoter sequence. A UV induced promoter. When the host cell is exposed in UV, the promoter will be actived, and the transcription of downstram gene will be started. false false _2085_ 27665 27665 9 false The sequence of the RecA promoter in this part is from BB_J22106 in igem. false Jinyan Chu BBa_K1667002 1 BBa_K1667002 opdA coding sequence 2015-09-06T11:00:00Z 2015-09-07T06:42:38Z The sequence of the opdA gene in this part is from BB_K215090, BB_K215091, and BB_K1010008 in igem. A DNA sequence coding opdA protein which has the alility to degrade organophosphorus pesticide. false false _2085_ 27665 27665 9 false There is a ompA signal peptide in front of the opdA gene. The ompA piptide could lead the expressed opdA protein out of the host cells. false Jinyan Chu BBa_K1667002_sequence 1 atgaaaaaaaccgctatcgcgatcgcagttgcactggctggtttcgctaccgttgcgcaggccaagcttggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagtga BBa_K1667004_sequence 1 taattttgtttaactttaagaaggagatataccaagcttatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa BBa_K1667003_sequence 1 cgaacagaaagtaatcgtattgtacacggccgcataatcgaactagaaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttcaacagaacatattgactatccggtattacccggcatgacaggagtaaaaatggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctccatcatgtaataa BBa_K2112015_sequence 1 gaattccgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcaagctttactagatgaaaaaaaccgctatcgcgatcgcagttgcactggctggtttcgctaccgttgcgcaggccaagcttggcgatctgatcaataccgtgcgcggcccgatcccggtgagcgaagcgggttttaccctgacccatgaacatatctgcggtagcagcgccggctttctgcgcgcctggccggagttctttggtagccgtaaagccctggccgaaaaagccgtgcgcggtctgcgtcacgcgcgtgccgcgggcgttcagaccatcgtggatgtgagcaccttcgatattggccgcgatgttcgtctgctggccgaagttagccgtgccgcggatgttcatattgtggcggccaccggtctgtggtttgatccgccgctgagcatgcgcatgcgtagcgtggaagaactgacccagtttttcctgcgtgaaattcagcatggcattgaagataccggcattcgtgcgggtattatcaaagtggccaccaccggcaaagccaccccgtttcaggaactggtgctgaaagcggcggcccgcgcgagcctggcgaccggcgtgccggtgaccacccataccagcgcgagccagcgcgatggcgaacagcaggcggcgatttttgaaagcgaaggcctgagcccgagccgtgtgtgcattggtcatagcgatgataccgatgatctgagctatctgaccggtctggcggcgcgtggttatctggtgggcctggatcgcatgccgtatagcgccattggcctggaaggtaatgcgagcgcgctggcgctgtttggcacccgtagctggcagacccgtgcgctgctgattaaagccctgattgatcgtggttataaagatcgcattctggtgagccatgattggctgtttggctttagcagctatgttaccaacatcatggatgtgatggatcgtattaatccggatggtatggcgtttgtgccgctgcgcgtgattccgttcctgcgtgaaaaaggtgtgccgccggaaaccctggcgggtgttaccgttgcgaatccggcgcgttttctgagcccgaccggcagcctcgagtgatactagagcgaacagaaagtaatcgtattgtacacggccgcataatcgaactagaaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttcaacagaacatattgactatccggtattacccggcatgacaggagtaaaaatggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggccagattgagaaacaatttggtaaaggctccatcatgtaataatactagagtaattttgtttaactttaagaaggagatataccaagcttatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataa BBa_K2112011_sequence 1 gaattccgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataattttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcaagctt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z