LOCUS       BBa_K2113002            1797 bp    DNA     linear       25-Nov-2024
DEFINITION  temperature sensitive system with lambda pr promoter under cI 
            regulation
ACCESSION   BBa_K2113002
VERSION     BBa_K2113002.1  
FEATURES             Location/Qualifiers
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     stem_loop       804..823
                     /label=T7 TE
     misc_feature    830..830
                     /label=stop
     polyA_site      824..837
                     /label=polya
     misc_feature    797..837
                     /label=BBa_B0012
     terminator      797..837
                     /label=BBa_B0012
     stem_loop       858..880
                     /label=stem_loop
     misc_feature    846..891
                     /label=BBa_B0011
     terminator      846..891
                     /label=BBa_B0011
     misc_feature    1..35
                     /label=BBa_J23114
     misc_feature    48..51
                     /label=conserved
     misc_feature    44..55
                     /label=BBa_B0034
     CDS             62..775
                     /label=cIts
     misc_feature    62..775
                     /label=BBa_K098997
     misc_feature    797..837
                     /label=BBa_B0012
     stem_loop       804..823
                     /label=T7 TE
     polyA_site      824..837
                     /label=polya
     misc_feature    830..830
                     /label=stop
     misc_feature    846..891
                     /label=BBa_B0011
     stem_loop       858..880
                     /label=stem_loop
     misc_feature    900..916
                     /label=OR2
     misc_feature    900..948
                     /label=BBa_R0051
     promoter        914..919
                     /label=-35
     misc_feature    924..940
                     /label=OR1
     promoter        937..942
                     /label=-10
     misc_feature    776..788
                     /label=conserved
     misc_feature    1..948
                     /label=BBa_K608351
     misc_feature    961..964
                     /label=conserved
     RBS             957..968
                     /label=BBa_B0034
     CDS             975..1694
                     /label=GFP protein
     CDS             975..1694
                     /label=BBa_E0040
     stem_loop       1710..1729
                     /label=T7 TE
     misc_feature    1736..1736
                     /label=stop
     polyA_site      1730..1743
                     /label=polya
     misc_feature    1703..1743
                     /label=BBa_B0012
     terminator      1703..1743
                     /label=BBa_B0012
     stem_loop       1764..1786
                     /label=stem_loop
     misc_feature    1752..1797
                     /label=BBa_B0011
     terminator      1752..1797
                     /label=BBa_B0011
     terminator      1703..1797
                     /label=BBa_B0014
     terminator      1703..1797
                     /label=BBa_K823017
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
      121 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
      181 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
      241 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
      301 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
      361 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
      421 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
      481 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
      541 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
      601 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
      661 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
      721 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gctgactcca
      781 gcggccgcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
      841 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt ttactagagt
      901 aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcta ctagagaaag
      961 aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     1021 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     1081 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     1141 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag
     1201 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
     1261 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
     1321 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     1381 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
     1441 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
     1501 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     1561 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     1621 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     1681 tatacaaata ataatactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1741 atatactaga gagagaatat aaaaagccag attattaatc cggctttttt attattt
//