BBa_K2113002
type: ComponentDefinition
persistent identity: BBa K2113002
display id: BBa_K2113002
version: 1
was generated by: iGEM to SBOL conversion
description: temperature sensitive system with lambda pr promoter under cI regulation
created: 2016-10-12T11:00:00Z
modified: 2016-10-14T12:42:39Z
mutable provenance: 2016 distribution kit
owned by: james
owned by: myers
mutable description: It is a construct which contains a complete temperature sensitive system with lambda pr as a promoter (BBa_K608351) activated at 42 degree celcius with gfp (BBa_E0040) as its reporter to study the expression at various temperatures.
discontinued: false
dominant: false
experience: experience/None
group u list: _2581_
m user id: 25971
owner id: 25970
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: nil
star: false
creator: SVCE_CHENNAI
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0012
component: BBa_B0034
component: BBa_K608351
component: BBa_B0011
component: BBa_E0040
component: BBa_K823017
sequence annotation: BBa_K608351
sequence annotation: BBa_B0034
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence annotation: BBa_E0040
sequence annotation: BBa_K823017
sequence: https://synbiohub.org/public/igem/BBa_K2113002_sequence/1

BBa_B0012
type: Component
persistent identity: component2500602
display id: component2500602
version: 1
top level: BBa_K2113002
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component2500597
display id: component2500597
version: 1
top level: BBa_K2113002
definition: BBa_B0034
access: public

BBa_K608351
type: Component
persistent identity: component2500613
display id: component2500613
version: 1
top level: BBa_K2113002
definition: BBa_K608351
access: public

BBa_B0011
type: Component
persistent identity: component2500606
display id: component2500606
version: 1
top level: BBa_K2113002
definition: BBa_B0011
access: public

BBa_E0040
type: Component
persistent identity: component2500617
display id: component2500617
version: 1
top level: BBa_K2113002
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component2500615
display id: component2500615
version: 1
top level: BBa_K2113002
definition: BBa_B0034
access: public

BBa_K823017
type: Component
persistent identity: component2500625
display id: component2500625
version: 1
top level: BBa_K2113002
definition: BBa_K823017
access: public

BBa_K608351
type: SequenceAnnotation
persistent identity: annotation2500613
display id: annotation2500613
version: 1
top level: BBa_K2113002
location: https://synbiohub.org/public/igem/BBa_K2113002/annotation2500613/range2500613/1
component: BBa_K608351

https://synbiohub.org/public/igem/BBa_K2113002/annotation2500613/range2500613/1
type: Range
persistent identity: range2500613
display id: range2500613
version: 1
top level: BBa_K2113002
direction: direction/reverse
start: 1
end: 948
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2500597
display id: annotation2500597
version: 1
top level: BBa_K2113002
location: https://synbiohub.org/public/igem/BBa_K2113002/annotation2500597/range2500597/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2113002/annotation2500597/range2500597/1
type: Range
persistent identity: range2500597
display id: range2500597
version: 1
top level: BBa_K2113002
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2500602
display id: annotation2500602
version: 1
top level: BBa_K2113002
location: https://synbiohub.org/public/igem/BBa_K2113002/annotation2500602/range2500602/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K2113002/annotation2500602/range2500602/1
type: Range
persistent identity: range2500602
display id: range2500602
version: 1
top level: BBa_K2113002
direction: direction/forward
start: 797
end: 837
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2500606
display id: annotation2500606
version: 1
top level: BBa_K2113002
location: https://synbiohub.org/public/igem/BBa_K2113002/annotation2500606/range2500606/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K2113002/annotation2500606/range2500606/1
type: Range
persistent identity: range2500606
display id: range2500606
version: 1
top level: BBa_K2113002
direction: direction/forward
start: 846
end: 891
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2500615
display id: annotation2500615
version: 1
top level: BBa_K2113002
location: https://synbiohub.org/public/igem/BBa_K2113002/annotation2500615/range2500615/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2113002/annotation2500615/range2500615/1
type: Range
persistent identity: range2500615
display id: range2500615
version: 1
top level: BBa_K2113002
direction: direction/reverse
start: 957
end: 968
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2500617
display id: annotation2500617
version: 1
top level: BBa_K2113002
location: https://synbiohub.org/public/igem/BBa_K2113002/annotation2500617/range2500617/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K2113002/annotation2500617/range2500617/1
type: Range
persistent identity: range2500617
display id: range2500617
version: 1
top level: BBa_K2113002
direction: direction/reverse
start: 975
end: 1694
orientation: inline

BBa_K823017
type: SequenceAnnotation
persistent identity: annotation2500625
display id: annotation2500625
version: 1
top level: BBa_K2113002
location: https://synbiohub.org/public/igem/BBa_K2113002/annotation2500625/range2500625/1
component: BBa_K823017

https://synbiohub.org/public/igem/BBa_K2113002/annotation2500625/range2500625/1
type: Range
persistent identity: range2500625
display id: range2500625
version: 1
top level: BBa_K2113002
direction: direction/reverse
start: 1703
end: 1797
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K608351
type: ComponentDefinition
persistent identity: BBa K608351
display id: BBa_K608351
version: 1
was generated by: iGEM to SBOL conversion
description: (correct) temperature sensitive promoter
created: 2011-09-13T11:00:00Z
modified: 2015-05-08T01:12:52Z
mutable provenance: asd
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: correct temperatursesitive promotor
discontinued: false
dominant: false
experience: experience/Works
group u list: _780_
m user id: 0
owner id: 9113
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: asd
star: false
creator: Theodhor Tiko
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: BBa_J23114
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: BBa_K098997
sequence annotation: cIts
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K608351_sequence/1

BBa_J23114
type: SequenceAnnotation
persistent identity: annotation2131300
display id: annotation2131300
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K608351/annotation2131300/range2131300/1
type: Range
persistent identity: range2131300
display id: range2131300
version: 1
top level: BBa_K608351
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2131302
display id: annotation2131302
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K608351/annotation2131302/range2131302/1
type: Range
persistent identity: range2131302
display id: range2131302
version: 1
top level: BBa_K608351
direction: direction/reverse
start: 44
end: 55
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2131301
display id: annotation2131301
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K608351/annotation2131301/range2131301/1
type: Range
persistent identity: range2131301
display id: range2131301
version: 1
top level: BBa_K608351
direction: direction/forward
start: 48
end: 51
orientation: inline

BBa_K098997
type: SequenceAnnotation
persistent identity: annotation2131304
display id: annotation2131304
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K608351/annotation2131304/range2131304/1
type: Range
persistent identity: range2131304
display id: range2131304
version: 1
top level: BBa_K608351
direction: direction/reverse
start: 62
end: 775
orientation: inline

cIts
type: SequenceAnnotation
persistent identity: annotation2131303
display id: annotation2131303
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K608351/annotation2131303/range2131303/1
type: Range
persistent identity: range2131303
display id: range2131303
version: 1
top level: BBa_K608351
direction: direction/forward
start: 62
end: 775
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2131425
display id: annotation2131425
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K608351/annotation2131425/range2131425/1
type: Range
persistent identity: range2131425
display id: range2131425
version: 1
top level: BBa_K608351
direction: direction/forward
start: 776
end: 788
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2131305
display id: annotation2131305
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K608351/annotation2131305/range2131305/1
type: Range
persistent identity: range2131305
display id: range2131305
version: 1
top level: BBa_K608351
direction: direction/forward
start: 797
end: 837
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2131306
display id: annotation2131306
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K608351/annotation2131306/range2131306/1
type: Range
persistent identity: range2131306
display id: range2131306
version: 1
top level: BBa_K608351
direction: direction/forward
start: 804
end: 823
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2131307
display id: annotation2131307
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_K608351/annotation2131307/range2131307/1
type: Range
persistent identity: range2131307
display id: range2131307
version: 1
top level: BBa_K608351
direction: direction/forward
start: 824
end: 837
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2131308
display id: annotation2131308
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K608351/annotation2131308/range2131308/1
type: Range
persistent identity: range2131308
display id: range2131308
version: 1
top level: BBa_K608351
direction: direction/forward
start: 830
end: 830
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2131309
display id: annotation2131309
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K608351/annotation2131309/range2131309/1
type: Range
persistent identity: range2131309
display id: range2131309
version: 1
top level: BBa_K608351
direction: direction/forward
start: 846
end: 891
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2131310
display id: annotation2131310
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K608351/annotation2131310/range2131310/1
type: Range
persistent identity: range2131310
display id: range2131310
version: 1
top level: BBa_K608351
direction: direction/forward
start: 858
end: 880
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2131312
display id: annotation2131312
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K608351/annotation2131312/range2131312/1
type: Range
persistent identity: range2131312
display id: range2131312
version: 1
top level: BBa_K608351
direction: direction/forward
start: 900
end: 948
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2131311
display id: annotation2131311
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K608351/annotation2131311/range2131311/1
type: Range
persistent identity: range2131311
display id: range2131311
version: 1
top level: BBa_K608351
direction: direction/forward
start: 900
end: 916
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2131313
display id: annotation2131313
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K608351/annotation2131313/range2131313/1
type: Range
persistent identity: range2131313
display id: range2131313
version: 1
top level: BBa_K608351
direction: direction/forward
start: 914
end: 919
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2131314
display id: annotation2131314
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K608351/annotation2131314/range2131314/1
type: Range
persistent identity: range2131314
display id: range2131314
version: 1
top level: BBa_K608351
direction: direction/forward
start: 924
end: 940
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2131315
display id: annotation2131315
version: 1
top level: BBa_K608351
location: https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K608351/annotation2131315/range2131315/1
type: Range
persistent identity: range2131315
display id: range2131315
version: 1
top level: BBa_K608351
direction: direction/forward
start: 937
end: 942
orientation: inline

BBa_K823017
type: ComponentDefinition
persistent identity: BBa K823017
display id: BBa_K823017
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2012-09-10T11:00:00Z
modified: 2015-05-08T01:13:30Z
mutable provenance: Part:BBa_B0014
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is a copy of the Part:B0014. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This is a copy of the Part:B0014. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
star: false
creator: Jara Radeck
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0014
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K823017_sequence/1

BBa_B0014
type: Component
persistent identity: component2182657
display id: component2182657
version: 1
top level: BBa_K823017
definition: BBa_B0014
access: public

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2182657
display id: annotation2182657
version: 1
top level: BBa_K823017
location: https://synbiohub.org/public/igem/BBa_K823017/annotation2182657/range2182657/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K823017/annotation2182657/range2182657/1
type: Range
persistent identity: range2182657
display id: range2182657
version: 1
top level: BBa_K823017
direction: direction/reverse
start: 1
end: 95
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

https://synbiohub.org/public/igem/BBa_K2113002_sequence/1
type: Sequence
persistent identity: BBa K2113002 sequence
display id: BBa_K2113002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgactccagcggccgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K608351_sequence/1
type: Sequence
persistent identity: BBa K608351 sequence
display id: BBa_K608351_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgactccagcggccgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattttactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823017_sequence/1
type: Sequence
persistent identity: BBa K823017 sequence
display id: BBa_K823017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z