BBa_K2113007
type: ComponentDefinition
persistent identity: BBa K2113007
display id: BBa_K2113007
version: 1
was generated by: iGEM to SBOL conversion
description: lac inducible AMP without glycine
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T03:41:51Z
mutable provenance: 2016 distribution kit (BBa_K314103)
owned by: james
owned by: myers
mutable description: It consists of the anti-microbial peptides (AMP) without glycine residues (BBa_K2113004) under the regulation of the lac inducible cassette (BBa_K314103).The expression of the AMPs can be controlled by varying the concentration of IPTG (lactose)
discontinued: false
dominant: false
experience: experience/None
group u list: _2581_
m user id: 25972
owner id: 25972
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: nil
star: false
creator: SVCE_CHENNAI
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K823017
component: BBa_K592009
component: BBa_B0034
component: BBa_K2113004
component: BBa_K314103
sequence annotation: BBa_K314103
sequence annotation: BBa_K2113004
sequence annotation: BBa_B0034
sequence annotation: BBa_K592009
sequence annotation: BBa_K823017
sequence: https://synbiohub.org/public/igem/BBa_K2113007_sequence/1

BBa_K823017
type: Component
persistent identity: component2501682
display id: component2501682
version: 1
top level: BBa_K2113007
definition: BBa_K823017
access: public

BBa_K592009
type: Component
persistent identity: component2501674
display id: component2501674
version: 1
top level: BBa_K2113007
definition: amilCP
access: public

BBa_B0034
type: Component
persistent identity: component2501672
display id: component2501672
version: 1
top level: BBa_K2113007
definition: BBa_B0034
access: public

BBa_K2113004
type: Component
persistent identity: component2501670
display id: component2501670
version: 1
top level: BBa_K2113007
definition: BBa_K2113004
access: public

BBa_K314103
type: Component
persistent identity: component2501669
display id: component2501669
version: 1
top level: BBa_K2113007
definition: BBa_K314103
access: public

BBa_K314103
type: SequenceAnnotation
persistent identity: annotation2501669
display id: annotation2501669
version: 1
top level: BBa_K2113007
location: https://synbiohub.org/public/igem/BBa_K2113007/annotation2501669/range2501669/1
component: BBa_K314103

https://synbiohub.org/public/igem/BBa_K2113007/annotation2501669/range2501669/1
type: Range
persistent identity: range2501669
display id: range2501669
version: 1
top level: BBa_K2113007
direction: direction/reverse
start: 1
end: 1638
orientation: inline

BBa_K2113004
type: SequenceAnnotation
persistent identity: annotation2501670
display id: annotation2501670
version: 1
top level: BBa_K2113007
location: https://synbiohub.org/public/igem/BBa_K2113007/annotation2501670/range2501670/1
component: BBa_K2113004

https://synbiohub.org/public/igem/BBa_K2113007/annotation2501670/range2501670/1
type: Range
persistent identity: range2501670
display id: range2501670
version: 1
top level: BBa_K2113007
direction: direction/reverse
start: 1645
end: 1668
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2501672
display id: annotation2501672
version: 1
top level: BBa_K2113007
location: https://synbiohub.org/public/igem/BBa_K2113007/annotation2501672/range2501672/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2113007/annotation2501672/range2501672/1
type: Range
persistent identity: range2501672
display id: range2501672
version: 1
top level: BBa_K2113007
direction: direction/reverse
start: 1677
end: 1688
orientation: inline

BBa_K592009
type: SequenceAnnotation
persistent identity: annotation2501674
display id: annotation2501674
version: 1
top level: BBa_K2113007
location: https://synbiohub.org/public/igem/BBa_K2113007/annotation2501674/range2501674/1
component: BBa_K592009

https://synbiohub.org/public/igem/BBa_K2113007/annotation2501674/range2501674/1
type: Range
persistent identity: range2501674
display id: range2501674
version: 1
top level: BBa_K2113007
direction: direction/reverse
start: 1695
end: 2363
orientation: inline

BBa_K823017
type: SequenceAnnotation
persistent identity: annotation2501682
display id: annotation2501682
version: 1
top level: BBa_K2113007
location: https://synbiohub.org/public/igem/BBa_K2113007/annotation2501682/range2501682/1
component: BBa_K823017

https://synbiohub.org/public/igem/BBa_K2113007/annotation2501682/range2501682/1
type: Range
persistent identity: range2501682
display id: range2501682
version: 1
top level: BBa_K2113007
direction: direction/reverse
start: 2372
end: 2466
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K314103
type: ComponentDefinition
persistent identity: BBa K314103
display id: BBa_K314103
version: 1
was generated by: iGEM to SBOL conversion
description: Lac induced expression cassette
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: construct
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lac induced protein expression insert includes f1 origin, Lac I gene and RBS
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
star: false
creator: Tomas Huber, Justin Siegel
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K314111
component: BBa_K314110
component: BBa_B0034
component: BBa_R0011
sequence annotation: BBa_K314110
sequence annotation: BBa_K314111
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K314103_sequence/1

BBa_K314111
type: Component
persistent identity: component2091082
display id: component2091082
version: 1
top level: BBa_K314103
definition: BBa_K314111
access: public

BBa_K314110
type: Component
persistent identity: component2091081
display id: component2091081
version: 1
top level: BBa_K314103
definition: f1 ori
access: public

BBa_B0034
type: Component
persistent identity: component2091089
display id: component2091089
version: 1
top level: BBa_K314103
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2091083
display id: component2091083
version: 1
top level: BBa_K314103
definition: lacI+pL
access: public

BBa_K314110
type: SequenceAnnotation
persistent identity: annotation2091081
display id: annotation2091081
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091081/range2091081/1
component: BBa_K314110

https://synbiohub.org/public/igem/BBa_K314103/annotation2091081/range2091081/1
type: Range
persistent identity: range2091081
display id: range2091081
version: 1
top level: BBa_K314103
direction: direction/reverse
start: 1
end: 455
orientation: inline

BBa_K314111
type: SequenceAnnotation
persistent identity: annotation2091082
display id: annotation2091082
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091082/range2091082/1
component: BBa_K314111

https://synbiohub.org/public/igem/BBa_K314103/annotation2091082/range2091082/1
type: Range
persistent identity: range2091082
display id: range2091082
version: 1
top level: BBa_K314103
direction: direction/reverse
start: 464
end: 1555
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2091083
display id: annotation2091083
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091083/range2091083/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K314103/annotation2091083/range2091083/1
type: Range
persistent identity: range2091083
display id: range2091083
version: 1
top level: BBa_K314103
direction: direction/forward
start: 1564
end: 1617
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2091089
display id: annotation2091089
version: 1
top level: BBa_K314103
location: https://synbiohub.org/public/igem/BBa_K314103/annotation2091089/range2091089/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K314103/annotation2091089/range2091089/1
type: Range
persistent identity: range2091089
display id: range2091089
version: 1
top level: BBa_K314103
direction: direction/reverse
start: 1627
end: 1638
orientation: inline

BBa_K2113004
type: ComponentDefinition
persistent identity: BBa K2113004
display id: BBa_K2113004
version: 1
was generated by: iGEM to SBOL conversion
description: AMP without glycine
created: 2016-10-13T11:00:00Z
modified: 2016-10-14T12:43:35Z
mutable provenance: nil
owned by: james
owned by: myers
mutable description: It consists of cationic anti-microbial peptides (AMP) with consecutive sequences of arginine and tryptophan (WRWRWR).This can be used as an antimicrobial agent against a wide range of gram positive and gram negative bacteria.
discontinued: false
dominant: false
experience: experience/None
group u list: _2581_
m user id: 25971
owner id: 25972
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: nil
star: false
creator: SVCE_CHENNAI
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2113004_sequence/1

amilCP
type: ComponentDefinition
persistent identity: BBa K592009
display id: BBa_K592009
version: 1
was generated by: iGEM to SBOL conversion
description: amilCP, blue chromoprotein
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Acropora millepora
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This chromoprotein, amilCP, naturally exhibits very strong color when expressed. The color is blue/purple and is visible to naked eye, thereby requiring no instruments to observe. This DNA was provided by Jeffrey Miller at UCLA. It was made BioBrick-compatible after removal of one illegal internal restriction site (EcoRI).
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Illegal internal restriction site had to be removed (EcoRI).
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: amilCP
sequence: https://synbiohub.org/public/igem/BBa_K592009_sequence/1

amilCP
type: SequenceAnnotation
persistent identity: annotation2131628
display id: annotation2131628
version: 1
top level: amilCP
location: https://synbiohub.org/public/igem/BBa_K592009/annotation2131628/range2131628/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K592009/annotation2131628/range2131628/1
type: Range
persistent identity: range2131628
display id: range2131628
version: 1
top level: amilCP
direction: direction/forward
start: 1
end: 666
orientation: inline

BBa_K823017
type: ComponentDefinition
persistent identity: BBa K823017
display id: BBa_K823017
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2012-09-10T11:00:00Z
modified: 2015-05-08T01:13:30Z
mutable provenance: Part:BBa_B0014
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is a copy of the Part:B0014. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1081_
m user id: 0
owner id: 11555
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This is a copy of the Part:B0014. Only here the part is cloned in the standard vector pSB1C3 instead of the pSB1AK3.
star: false
creator: Jara Radeck
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0014
sequence annotation: BBa_B0014
sequence: https://synbiohub.org/public/igem/BBa_K823017_sequence/1

BBa_B0014
type: Component
persistent identity: component2182657
display id: component2182657
version: 1
top level: BBa_K823017
definition: BBa_B0014
access: public

BBa_B0014
type: SequenceAnnotation
persistent identity: annotation2182657
display id: annotation2182657
version: 1
top level: BBa_K823017
location: https://synbiohub.org/public/igem/BBa_K823017/annotation2182657/range2182657/1
component: BBa_B0014

https://synbiohub.org/public/igem/BBa_K823017/annotation2182657/range2182657/1
type: Range
persistent identity: range2182657
display id: range2182657
version: 1
top level: BBa_K823017
direction: direction/reverse
start: 1
end: 95
orientation: inline

BBa_B0014
type: ComponentDefinition
persistent identity: BBa B0014
display id: BBa_B0014
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0012-B0011)
created: 2003-07-15T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0012 and BBa_B0011
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_B0014_sequence/1

BBa_B0011
type: Component
persistent identity: component939311
display id: component939311
version: 1
top level: BBa_B0014
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component939303
display id: component939303
version: 1
top level: BBa_B0014
definition: BBa_B0012
access: public

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation939303
display id: annotation939303
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0014/annotation939303/range939303/1
type: Range
persistent identity: range939303
display id: range939303
version: 1
top level: BBa_B0014
direction: direction/forward
start: 1
end: 41
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation939311
display id: annotation939311
version: 1
top level: BBa_B0014
location: https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_B0014/annotation939311/range939311/1
type: Range
persistent identity: range939311
display id: range939311
version: 1
top level: BBa_B0014
direction: direction/forward
start: 50
end: 95
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

f1 ori
type: ComponentDefinition
persistent identity: BBa K314110
display id: BBa_K314110
version: 1
was generated by: iGEM to SBOL conversion
description: f1 origin
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: Pet 29 vector
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Phage origin recognized by M13. Used to make SS DNA when infected with M13k07 helper phage
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
star: false
creator: IGEM 2010 Team Washington
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: f1 origin
sequence: https://synbiohub.org/public/igem/BBa_K314110_sequence/1

f1 origin
type: SequenceAnnotation
persistent identity: annotation2115768
display id: annotation2115768
version: 1
top level: f1 ori
location: https://synbiohub.org/public/igem/BBa_K314110/annotation2115768/range2115768/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K314110/annotation2115768/range2115768/1
type: Range
persistent identity: range2115768
display id: range2115768
version: 1
top level: f1 ori
direction: direction/forward
start: 18
end: 324
orientation: inline

BBa_K314111
type: ComponentDefinition
persistent identity: BBa K314111
display id: BBa_K314111
version: 1
was generated by: iGEM to SBOL conversion
description: Lac I
created: 2010-10-07T11:00:00Z
modified: 2015-05-08T01:11:55Z
mutable provenance: Pet 29 vector
owned by: james
owned by: myers
mutable description: Lac I gene with promoter and RBS
discontinued: false
dominant: false
experience: experience/Works
group u list: _496_
m user id: 0
owner id: 6395
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: IGEM 2010 Team Washington
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_K314111_sequence/1

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K2113007_sequence/1
type: Sequence
persistent identity: BBa K2113007 sequence
display id: BBa_K2113007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgtggcgttggcgttggcgttaatactagagaaagaggagaaatactagatgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtact…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K314103_sequence/1
type: Sequence
persistent identity: BBa K314103 sequence
display id: BBa_K314103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2113004_sequence/1
type: Sequence
persistent identity: BBa K2113004 sequence
display id: BBa_K2113004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtggcgttggcgttggcgttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592009_sequence/1
type: Sequence
persistent identity: BBa K592009 sequence
display id: BBa_K592009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtgatcgctaaacaaatgacctacaaggtttatatgtcaggcacggtcaatggacactactttgaggtcgaaggcgatggaaaaggtaagccctacgagggggagcagacggtaaagctcactgtcaccaagggcggacctctgccatttgcttgggatattttatcaccacagtgtcagtacggaagcataccattcaccaagtaccctgaagacatccctgactatgtaaagcagtcattcccggagggctatacatgggagaggatcatgaactttgaagatggtgcagtgtgtactgtcagcaatgattccagcatccaaggcaactgtttcatctaccatgtcaagttctctggtttgaactttcctcccaatggacctgtcatgcagaagaagacacagggctgggaacccaacactgagcgtctctttgcacgagatggaatgctgctaggaaacaactttatggctctgaagttagaaggaggcggtcactatttgtgtgaatttaaaactacttacaaggcaaagaagcctgtgaagatgccagggtatcactatgttgaccgcaaactggatgtaaccaatcacaacaaggattacacttcggttgagcagtgtgaaatttccattgcacgcaaacctgtggtcgcctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K823017_sequence/1
type: Sequence
persistent identity: BBa K823017 sequence
display id: BBa_K823017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0014_sequence/1
type: Sequence
persistent identity: BBa B0014 sequence
display id: BBa_B0014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K314110_sequence/1
type: Sequence
persistent identity: BBa K314110 sequence
display id: BBa_K314110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K314111_sequence/1
type: Sequence
persistent identity: BBa K314111 sequence
display id: BBa_K314111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggacatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z