LOCUS       BBa_K2113008            2472 bp    DNA     linear       23-Dec-2024
DEFINITION  lac inducible AMP with glycine
ACCESSION   BBa_K2113008
VERSION     BBa_K2113008.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     CDS             1645..1674
                     /label=BBa_K2113005
     misc_feature    1687..1690
                     /label=conserved
     RBS             1683..1694
                     /label=BBa_B0034
     CDS             1701..2366
                     /label=amilCP
     CDS             1701..2369
                     /label=BBa_K592009
     stem_loop       2385..2404
                     /label=T7 TE
     misc_feature    2411..2411
                     /label=stop
     polyA_site      2405..2418
                     /label=polya
     misc_feature    2378..2418
                     /label=BBa_B0012
     terminator      2378..2418
                     /label=BBa_B0012
     stem_loop       2439..2461
                     /label=stem_loop
     misc_feature    2427..2472
                     /label=BBa_B0011
     terminator      2427..2472
                     /label=BBa_B0011
     terminator      2378..2472
                     /label=BBa_B0014
     terminator      2378..2472
                     /label=BBa_K823017
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaata ctagatgggc ggctggcgtt ggcgttggcg ttaatactag
     1681 agaaagagga gaaatactag atgagtgtga tcgctaaaca aatgacctac aaggtttata
     1741 tgtcaggcac ggtcaatgga cactactttg aggtcgaagg cgatggaaaa ggtaagccct
     1801 acgaggggga gcagacggta aagctcactg tcaccaaggg cggacctctg ccatttgctt
     1861 gggatatttt atcaccacag tgtcagtacg gaagcatacc attcaccaag taccctgaag
     1921 acatccctga ctatgtaaag cagtcattcc cggagggcta tacatgggag aggatcatga
     1981 actttgaaga tggtgcagtg tgtactgtca gcaatgattc cagcatccaa ggcaactgtt
     2041 tcatctacca tgtcaagttc tctggtttga actttcctcc caatggacct gtcatgcaga
     2101 agaagacaca gggctgggaa cccaacactg agcgtctctt tgcacgagat ggaatgctgc
     2161 taggaaacaa ctttatggct ctgaagttag aaggaggcgg tcactatttg tgtgaattta
     2221 aaactactta caaggcaaag aagcctgtga agatgccagg gtatcactat gttgaccgca
     2281 aactggatgt aaccaatcac aacaaggatt acacttcggt tgagcagtgt gaaatttcca
     2341 ttgcacgcaa acctgtggtc gcctaataat actagagtca cactggctca ccttcgggtg
     2401 ggcctttctg cgtttatata ctagagagag aatataaaaa gccagattat taatccggct
     2461 tttttattat tt
//