BBa_K2114001 1 BBa_K2114001 aGFPnano_HA_aHelix_cotZ 2016-10-05T11:00:00Z 2016-10-19T02:50:21Z Amplification of cotZ from genomic DNA of Bacillus subtilis. The anti-GFP nanobody was provided by Dr. Maximilian Ulbrich (BIOSS) N-terminal fusion of anti-GFP nanobody to spore crust gene cotZ. false false _2582_ 32299 32299 9 true Introduction of HA epitope tag and alpha helical linker by primer extensions. false Wladislaw Stroukov annotation2491416 1 cotZ range2491416 1 415 861 annotation2491413 1 anti-GFP nanobody range2491413 1 1 354 annotation2491414 1 HA range2491414 1 355 381 annotation2491415 1 alpha helical linker range2491415 1 382 414 BBa_K2114001_sequence 1 atgacaagtcaagtccaactggtggagtctggtggcgctttggtgcagccaggtggctctctgcgtttgtcctgtgccgcttctggcttcccagtgaaccgctattccatgcgctggtatcgccaggctccaggcaaagagcgtgagtgggtagccggtatgtccagcgcgggtgatcgtagctcctatgaagactccgtgaagggccgtttcaccatcagccgtgacgatgcccgtaacacggtgtatctgcaaatgaacagcttgaaacctgaagatacggccgtgtattactgtaatgtgaacgtgggcttcgagtattggggccaaggcacccaggtcaccgtctccagctacccatacgatgttccagattacgctggcggcggcgaagcggcggcgaaaggcggcggcatgagccagaaaacatcaagctgcgtgcgtgaagctgtagaaaatattgaagatctgcaaaacgctgttgaagaagactgcccgaccggctgccactctaagcttttatctgtaagccattcgttaggcgacacagtgccttttgcaatatttacatcaaaatcaacgccattagtcgccttcggaaatgtcggcgaactcgataacggcccttgctttaatacagtatttttcagggtcgaaagagtgcatggaagctgtgcaacactgtcattattaatcgcatttgacgaacacaaacacattttggacttcaccgataaagatacggtgtgtgaagtgttccgactcgaaaaaacgaactactgtattgaagttgacttagactgcttctgcgcaatcaactgcttaaatcctcgattaatcaatcgtacacatcatcattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z