BBa_K2114002 1 BBa_K2114002 aGFPnano_HA_G4S_cotZ 2016-10-05T11:00:00Z 2016-10-19T03:31:55Z The cotZ gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived camelid heavy-chain antibodies. N-terminal fusion of anti-GFP nanobody to spore crust gene cotZ by a flexible GGGGS linker. false false _2582_ 32299 32299 9 true Introduction of a HA epitope tag and a flexible GGGGS linker by primer extensions. false Wladislaw Stroukov annotation2491627 1 anti-GFP nanobody range2491627 1 1 354 annotation2491629 1 GGGGS linker range2491629 1 382 396 annotation2491630 1 cotZ range2491630 1 397 843 annotation2491628 1 HA range2491628 1 355 381 BBa_K2114002_sequence 1 atgacaagtcaagtccaactggtggagtctggtggcgctttggtgcagccaggtggctctctgcgtttgtcctgtgccgcttctggcttcccagtgaaccgctattccatgcgctggtatcgccaggctccaggcaaagagcgtgagtgggtagccggtatgtccagcgcgggtgatcgtagctcctatgaagactccgtgaagggccgtttcaccatcagccgtgacgatgcccgtaacacggtgtatctgcaaatgaacagcttgaaacctgaagatacggccgtgtattactgtaatgtgaacgtgggcttcgagtattggggccaaggcacccaggtcaccgtctccagctacccatacgatgttccagattacgctggtggtggtggttctatgagccagaaaacatcaagctgcgtgcgtgaagctgtagaaaatattgaagatctgcaaaacgctgttgaagaagactgcccgaccggctgccactctaagcttttatctgtaagccattcgttaggcgacacagtgccttttgcaatatttacatcaaaatcaacgccattagtcgccttcggaaatgtcggcgaactcgataacggcccttgctttaatacagtatttttcagggtcgaaagagtgcatggaagctgtgcaacactgtcattattaatcgcatttgacgaacacaaacacattttggacttcaccgataaagatacggtgtgtgaagtgttccgactcgaaaaaacgaactactgtattgaagttgacttagactgcttctgcgcaatcaactgcttaaatcctcgattaatcaatcgtacacatcatcattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z