BBa_K2114009 1 BBa_K2114009 aGFPnano_HA_aHelix_cotG 2016-10-07T11:00:00Z 2016-10-19T09:03:30Z The cotG gene comes from the genome of B. subtilis. The anti-GFP nanobody is derived from camelid heavy-chain antibodies. N-terminal fusion of anti-GFP nanobody to spore crust gene cotG by a alpha helical linker. false false _2582_ 32299 32299 9 false Introduction of HA epitope tag and alpha helical linker by primer extensions and PCR. false Wladislaw Stroukov annotation2519187 1 anti-GFP nanobody range2519187 1 1 354 annotation2519564 1 alpha helical linker range2519564 1 382 414 annotation2519682 1 cotG range2519682 1 415 1002 annotation2519391 1 HA range2519391 1 355 381 BBa_K2114009_sequence 1 atgacaagtcaagtccaactggtggagtctggtggcgctttggtgcagccaggtggctctctgcgtttgtcctgtgccgcttctggcttcccagtgaaccgctattccatgcgctggtatcgccaggctccaggcaaagagcgtgagtgggtagccggtatgtccagcgcgggtgatcgtagctcctatgaagactccgtgaagggccgtttcaccatcagccgtgacgatgcccgtaacacggtgtatctgcaaatgaacagcttgaaacctgaagatacggccgtgtattactgtaatgtgaacgtgggcttcgagtattggggccaaggcacccaggtcaccgtctccagctacccatacgatgttccagattacgctggcggcggcgaagcggcggcgaaaggcggcggcttgggccactattcccattctgacatcgaagaagcggtgaaatccgcaaaaaaagaaggtttaaaggattatttataccaagagcctcatggaaaaaaacgcagtcataaaaagtcgcaccgcactcacaaaaaatctcgcagccataaaaaatcatactgctctcacaaaaaatctcgcagtcacaaaaaatcattctgttctcacaaaaaatctcgcagccacaaaaaatcatactgctctcacaagaaatctcgcagccacaaaaaatcgtaccgttctcacaaaaaatctcgcagctataaaaaatcttaccgttcttacaaaaaatctcgtagctataaaaaatcttgccgttcttacaaaaaatctcgcagctacaaaaagtcttactgttctcacaagaaaaaatctcgcagctataagaagtcatgccgcacacacaaaaaatcttatcgttcccataagaaatactacaaaaaaccgcaccaccactgcgacgactacaaaagacacgatgattatgacagcaaaaaagaatactggaaagacggcaattgctgggtagtcaaaaagaaatacaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z