BBa_K2114013 1 BBa_K2114013 GST_HA_G4S_CotB 2016-10-08T11:00:00Z 2016-10-12T04:11:49Z The cotB gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare). N-terminal fusion of glutathione S-transferase to spore crust gene cotB by a flexible GGGGS linker. false false _2582_ 32299 32299 9 false Introduction of HA epitope tag and GGGGS linker by primer extensions and PCR. false Wladislaw Stroukov annotation2495249 1 GGGGS linker range2495249 1 727 741 annotation2495242 1 glutathione S-transferase range2495242 1 1 699 annotation2495247 1 HA range2495247 1 700 726 annotation2495250 1 cotB range2495250 1 742 1884 BBa_K2114013_sequence 1 atgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatctggaagttctgttccaggggcccctgggatccccggaatacccatacgatgttccagattacgctggtggtggtggttctatgagcaagaggagaatgaaatatcattcaaataatgaaatatcgtattataactttttgcactcaatgaaagataaaattgttactgtatatcgtggaggtccggaatctaaaaaaggaaaattaacagctgtaaaatcagattatatagctttacaagctgaaaaaaaaataatttattatcagttggagcatgtgaaaagtattactgaggataccaataatagcaccacaacaattgagactgaggaaatgctcgatgctgatgattttcatagcttaatcggacatttaataaaccaatcagttcaatttaaccaagggggtccggaatctaaaaaaggaagattggtctggctgggagatgattacgctgcgttaaacacaaatgaggatggggtagtgtattttaatatccatcacatcaaaagtataagtaaacacgagcctgatttgaaaatagaagagcagacgccagttggagttttggaagctgatgatttaagcgaggtttttaagagtctgactcataaatgggtttcaattaatcgtggaggtccggaagccattgagggtatccttgtagataatgccgacggccattatactatagtgaaaaatcaagaggtgcttcgcatctatccttttcacataaaaagcatcagcttaggtccaaaagggtcgtacaaaaaagaggatcaaaaaaatgaacaaaaccaggaagacaataatgataaggacagcaattcgttcatttcttcaaaatcatatagctcatcaaaatcatctaaacgatcactaaaatcttcagatgatcaatcatccaaatctggtcgttcgtcacgttcaaaaagttcttcaaaatcatctaaacgatcactaaaatcttcggattatcaatcatccaaatctggccgttcgtcacgttcaaaaagttcttcaaaatcatctaaacgatcattaaaatcttcagattatcaatcatcaaaatcatctaaacgatcaccaagatcttcagattatcaatcatcaagatcaccaggctattcaagttcaataaaaagttcaggaaaacaaaaggaagattatagctatgaaacgattgtcagaacgatagactatcactggaaacgtaaattttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z